Restriction map for
pF1KB7123
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# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflII AflIII AgeI AhdI AlwNI
Aor51HI ApaI AscI AseI AsnI
Asp700I Asp718I AspEI AvaI AvrII
BbrPI BbsI BbvCI BcgI BciVI
BfrI BglII BlnI BlpI BpmI
Bpu1102I BpuAI BsaAI BsaBI BsaWI
BseAI BseRI BsiEI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrFI BsrGI BssHII
BssSI BstBI BstEII BstPI BstUI
CelII Cfr10I ClaI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FseI HgaI HincII HindII HpaI
KpnI KspI MaeII MaeIII MamI
MfeI MluI MroI MseI MunI
MvnI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI SacI SacII SalI
SapI SbfI SexAI SfiI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TaqI Tru9I Tsp45I TthHB8I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccI 813 GTATAC GT^[AC][GT]AC
AcsI 594 GAATTT [GA]^AATT[CT]
AcyI 406 GGCGCC G[GA]^CG[CT]C
Alw44I 502 GTGCAC G^TGCAC
AocI 148 CCTCAGG CC^T[ACGT]AGG
ApaLI 502 GTGCAC G^TGCAC
ApoI 594 GAATTT [GA]^AATT[CT]
AspHI 502 GTGCAC G[AT]GC[AT]^C
AspI 429 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 779 TGCGCA TGC^GCA
BalI 461 TGGCCA TGG^CCA
BamHI 114 GGATCC G^GATCC
BbeI 406 GGCGCC GGCGC^C
BclI 344 TGATCA T^GATCA
BfaI 340 CTAG C^TAG
BsaHI 406 GGCGCC G[GA]^CG[CT]C
BsaI 335 GAGACC GGTCTC(1/5)
BsiHKAI 502 GTGCAC G[AT]GC[AT]^C
BspMI 4 GCAGGT ACCTGC(4/8)
BsrDI 232 CATTGC GCAATG(2/0)
Bst1107I 813 GTATAC GTA^TAC
BstYI 114 GGATCC [GA]^GATC[CT]
BstZ17I 813 GTATAC GTA^TAC
Bsu36I 148 CCTCAGG CC^T[ACGT]AGG
CpoI 19 CGGACCG CG^G[AT]CCG
EaeI 461 TGGCCA [CT]^GGCC[GA]
Eco81I 148 CCTCAGG CC^T[ACGT]AGG
EcoNI 458 CCTTGGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 458 CCTTGG C^C[AT][AT]GG
FbaI 344 TGATCA T^GATCA
FspI 779 TGCGCA TGC^GCA
HaeII 406 GGCGCC [GA]GCGC^[CT]
Hin1I 406 GGCGCC G[GA]^CG[CT]C
HindIII 754 AAGCTT A^AGCTT
KasI 406 GGCGCC G^GCGCC
MaeI 340 CTAG C^TAG
MflI 114 GGATCC [GA]^GATC[CT]
MluNI 461 TGGCCA TGG^CCA
MscI 461 TGGCCA TGG^CCA
MslI 495 CACTGCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 406 GGCGCC GG^CGCC
PflFI 429 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 357 CCATCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 178 CAGCTG CAG^CTG
RsrII 19 CGGACCG CG^G[AT]CCG
ScaI 140 AGTACT AGT^ACT
SfoI 406 GGCGCC GGC^GCC
StyI 458 CCTTGG C^C[AT][AT]GG
Tsp509I 595 AATT ^AATT
Tth111I 429 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 357 CCATCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 560 CCAAGTACACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 114 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AlwI 115 GATCC GGATC(4/5)
114 GGATC
BanI 406 GGCGCC G^G[CT][GA]CC
556 GGTGCC
BanII 396 GAGCCC G[GA]GC[CT]^C
574 GGGCTC
BglI 398 GCCCAGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
508 GCCCTGCTGGC
BsrBI 166 CCGCTC CCGCTC(-3/-3)
927 CCGCTC
BstAPI 504 GCACGCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
718 GCAGCCTATGC
BstXI 323 CCAGTTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
464 CCAGGGAAGTGG
CfoI 407 GCGC GCG^C
780 GCGC
DdeI 149 CTCAG C^T[ACGT]AG
415 CTGAG
DpnII 115 GATC ^GATC
345 GATC
EarI 106 CTCTTC CTCTTC(1/4)
190 CTCTTC
FauI 878 CCCGC CCCGC(4/6)
824 GCGGG
HhaI 407 GCGC GCG^C
780 GCGC
HinP1I 407 GCGC G^CGC
780 GCGC
HphI 248 TCACC GGTGA(8/7)
257 TCACC
Ksp632I 106 CTCTTC CTCTTC(1/4)
190 CTCTTC
MboI 115 GATC ^GATC
345 GATC
MboII 107 TCTTC GAAGA(8/7)
191 TCTTC
MspA1I 178 CAGCTG C[AC]G^C[GT]G
822 CAGCGG
NdeII 115 GATC ^GATC
345 GATC
PpuMI 6 AGGTCCC [GA]G^G[AT]CC[CT]
535 GGGACCT
Sau3AI 115 GATC ^GATC
345 GATC
SfaNI 912 GATGC GCATC(5/9)
314 GCATC
StuI 51 AGGCCT AGG^CCT
75 AGGCCT
TfiI 661 GATTC G^A[AT]TC
854 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AciI AfaI AluI AvaII BbvI
BcnI BmyI BsaJI BsgI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DraII DsaI EcoO109I EcoRII Fnu4HI
FokI HaeIII HapII HinfI HpaII
ItaI MnlI MspI MvaI MwoI
NciI NlaIII NlaIV PleI PstI
RsaI Sau96I ScrFI SfcI TseI
TspRI