Restriction map for
pF1KB7121
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# NO CUT ENZYMES ( 150 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BalI BanI BbeI BbrPI
BbvCI BcgI BciVI BfaI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaI BseAI BsgI
BsiEI BsiWI BsmAI BsmBI BsmI
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BssHII Bst1107I
BstAPI BstBI BstUI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraII DraIII DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO109I EcoRI EcoT22I FseI FspI
HaeIII HincII HindII HindIII HpaI
KasI KpnI KspI MaeI MamI
MfeI MluI MluNI MroI MscI
MslI MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacII
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
TfiI Tth111I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AcyI 727 GGCGTC G[GA]^CG[CT]C
AlwNI 54 CAGGGACTG CAG[ACGT][ACGT][ACGT]^CTG
AvaI 632 CCCGGG C^[CT]CG[GA]G
BamHI 563 GGATCC G^GATCC
BbsI 504 GTCTTC GAAGAC(2/6)
BclI 575 TGATCA T^GATCA
BpuAI 504 GTCTTC GAAGAC(2/6)
BsaHI 727 GGCGTC G[GA]^CG[CT]C
BsaWI 371 ACCGGA [AT]^CCGG[AT]
BsoBI 632 CCCGGG C^[CT]CG[GA]G
Bsp1407I 906 TGTACA T^GTACA
BsrGI 906 TGTACA T^GTACA
BssSI 571 CTCGTG CACGAG(-5/-1)
BstEII 82 GGTGACC G^GT[ACGT]ACC
BstPI 82 GGTGACC G^GT[ACGT]ACC
BstYI 563 GGATCC [GA]^GATC[CT]
DsaI 595 CCACGG C^C[GA][CT]GG
EcoNI 648 CCTGGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 82 GGTGACC G^GT[ACGT]ACC
EcoRV 465 GATATC GAT^ATC
EcoT14I 881 CCTTGG C^C[AT][AT]GG
FauI 700 GCGGG CCCGC(4/6)
FbaI 575 TGATCA T^GATCA
HgaI 728 GCGTC GACGC(5/10)
Hin1I 727 GGCGTC G[GA]^CG[CT]C
HinfI 808 GAGTC G^A[ACGT]TC
MaeII 502 ACGT A^CGT
MflI 563 GGATCC [GA]^GATC[CT]
MseI 748 TTAA T^TAA
NlaIII 759 CATG CATG^
PflMI 53 CCAGGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 808 GAGTC GAGTC(4/5)
PstI 306 CTGCAG CTGCA^G
SapI 24 GAAGAGC GCTCTTC(1/4)
ScaI 220 AGTACT AGT^ACT
SmaI 632 CCCGGG CCC^GGG
SspBI 906 TGTACA T^GTACA
StyI 881 CCTTGG C^C[AT][AT]GG
TaqI 783 TCGA T^CGA
Tru9I 748 TTAA T^TAA
Tsp509I 317 AATT ^AATT
TthHB8I 783 TCGA T^CGA
Van91I 53 CCAGGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 542 CCATCTTGTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 563 GGATCC [GA]^GATC[CT]
XmaCI 632 CCCGGG C^CCGGG
XmaI 632 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AfaI 221 GTAC GT^AC
907 GTAC
AlwI 564 GATCC GGATC(4/5)
563 GGATC
BcnI 632 CCCGG CC^[GC]GG
633 CCGGG
BsrI 59 ACTGG ACTGG(1/-1)
559 ACTGG
BstXI 430 CCAAATGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
617 CCAACACTGTGG
Cac8I 377 GCTCGC GC[ACGT]^[ACGT]GC
900 GCTTGC
DpnII 564 GATC ^GATC
576 GATC
HaeII 538 AGCGCC [GA]GCGC^[CT]
644 AGCGCC
HapII 372 CCGG C^CGG
633 CCGG
HpaII 372 CCGG C^CGG
633 CCGG
MboI 564 GATC ^GATC
576 GATC
MspI 372 CCGG C^CGG
633 CCGG
MwoI 91 GCAGATACAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
103 GCCACAAGAGC
NciI 632 CCCGG CC^[GC]GG
633 CCGGG
NdeII 564 GATC ^GATC
576 GATC
RsaI 221 GTAC GT^AC
907 GTAC
Sau3AI 564 GATC ^GATC
576 GATC
SfcI 306 CTGCAG C^T[GA][CT]AG
518 CTATAG
#########################################
# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AciI AluI AspHI AvaII BanII
BbvI BmyI BpmI BsaJI BseRI
BsiHKAI BsiYI BslI BsmFI Bsp1286I
BssKI BstF5I BstNI CfoI Cfr13I
DdeI EarI EcoRII Fnu4HI FokI
HhaI HinP1I HphI ItaI Ksp632I
MaeIII MboII MnlI MspA1I MvaI
NlaIV SacI Sau96I ScrFI SfaNI
TseI Tsp45I TspRI