##################################
# NO CUT ENZYMES (  198 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflIII      AgeI        AhdI        Alw44I      
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AvaI        AviII       
AvrII       BalI        BamHI       BanI        BanII       
BbeI        BbrPI       BbsI        BbvCI       BcgI        
BclI        BcnI        BglI        BglII       BlnI        
BlpI        BmyI        BpmI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaHI       BsaI        BsaJI       
BsaWI       BseAI       BseRI       BsgI        BsiEI       
BsiHKAI     BsiWI       BsmBI       BsmFI       BsmI        
BsoBI       Bsp1286I    Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrFI       
BsrGI       BsrI        BssHII      BssKI       BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstF5I      
BstNI       BstPI       BstUI       BstXI       BstZ17I     
Bsu36I      CelII       CfoI        Cfr10I      ClaI        
CpoI        DraI        DraII       DraIII      DrdI        
DsaI        EaeI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRII      EcoT14I     
EcoT22I     FauI        FbaI        FokI        FseI        
FspI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HpaII       HphI        
KasI        KpnI        Ksp632I     KspI        MamI        
MfeI        MluI        MluNI       MroI        MscI        
MspA1I      MspI        MunI        MvaI        MvnI        
NaeI        NarI        NciI        NcoI        NdeI        
NgoMIV      NheI        NlaIV       NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        PvuII       RcaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScrFI       
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        Sse8387I    SspBI       
StuI        StyI        SwaI        TaqI        TfiI        
Tth111I     TthHB8I     Van91I      XcmI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AciI          423  CCGC                 CCGC(-3/-1)
AflII         262  CTTAAG               C^TTAAG
AlwI          690  GGATC                GGATC(4/5)
AvaII         370  GGTCC                G^G[AT]CC
BciVI         392  GGATAC               GTATCC(6/5)
BfrI          262  CTTAAG               C^TTAAG
BsiYI         672  CCAAATAAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          672  CCAAATAAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsrDI         415  GCAATG               GCAATG(2/0)
BstYI         690  GGATCT               [GA]^GATC[CT]
Cfr13I        370  GGTCC                G^G[ACGT]CC
DpnII         691  GATC                 ^GATC
EcoRV         705  GATATC               GAT^ATC
HincII        781  GTTAAC               GT[CT]^[GA]AC
HindII        781  GTTAAC               GT[CT]^[GA]AC
HindIII       588  AAGCTT               A^AGCTT
HinfI         271  GAGTC                G^A[ACGT]TC
HpaI          781  GTTAAC               GTT^AAC
MaeII         501  ACGT                 A^CGT
MaeIII        385  GTCAC                ^GT[ACGT]AC
MboI          691  GATC                 ^GATC
MflI          690  GGATCT               [GA]^GATC[CT]
MslI          148  CACCTACATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MwoI          860  GCTGTCCATGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeII         691  GATC                 ^GATC
NspI          408  GCATGC               [GA]CATG^[CT]
PleI          271  GAGTC                GAGTC(4/5)
Sau3AI        691  GATC                 ^GATC
Sau96I        370  GGTCC                G^G[ACGT]CC
ScaI          843  AGTACT               AGT^ACT
SfaNI         737  GCATC                GCATC(5/9)
SmlI          262  CTTAAG               C^T[CT][GA]AG
SphI          408  GCATGC               GCATG^C
SspI          745  AATATT               AAT^ATT
Tsp45I        385  GTCAC                ^GT[GC]AC
XbaI           52  TCTAGA               T^CTAGA
XhoII         690  GGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (    8 enzymes) #
###################################

AccI          257  GTCTAC               GT^[AC][GT]AC
              273  GTCTAC              
AfaI          767  GTAC                 GT^AC
              844  GTAC                
BfaI           53  CTAG                 C^TAG
              808  CTAG                
Cac8I         108  GCAAGC               GC[ACGT]^[ACGT]GC
              408  GCATGC              
DdeI          140  CTGAG                C^T[ACGT]AG
              404  CTCAG               
MaeI           53  CTAG                 C^TAG
              808  CTAG                
RsaI          767  GTAC                 GT^AC
              844  GTAC                
TspRI         380  CAGTG                CA[GC]TG[ACGT][ACGT]^
              758  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   15 enzymes) #
#########################################

AcsI        AluI        ApoI        BbvI        BsmAI       
Fnu4HI      ItaI        MboII       MnlI        MseI        
NlaIII      SfcI        Tru9I       TseI        Tsp509I