Restriction map for
pF1KB7103
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# NO CUT ENZYMES ( 154 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AflIII AgeI
AhdI Alw44I AlwI AlwNI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AvrII BamHI BanI
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BfrI BglII BlnI
BlpI BpmI Bpu1102I BpuAI BsaAI
BsaBI BsaWI BseAI BseRI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrGI BsrI
BssHII Bst1107I BstAPI BstBI BstYI
BstZ17I Bsu36I CelII ClaI CpoI
DpnII DraI DraII DrdI Eam1105I
EarI Eco47III Eco81I EcoNI EcoO109I
EcoRI EcoRV EcoT22I FbaI FseI
HindIII HinfI HpaI KasI KpnI
Ksp632I MaeII MamI MboI MfeI
MflI MluI MroI MseI MunI
NarI NdeI NdeII NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshBI Psp1406I PstI PvuI
RcaI RsaI RsrII SalI SapI
Sau3AI SbfI ScaI SexAI SfcI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI TfiI Tru9I
Tsp509I Tth111I TthHB8I XbaI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AvaII 80 GGTCC G^G[AT]CC
AviII 317 TGCGCA TGC^GCA
BfaI 202 CTAG C^TAG
BsaI 628 GAGACC GGTCTC(1/5)
BsgI 271 GTGCAG GTGCAG(16/14)
BsiEI 424 CGGCCG CG[GA][CT]^CG
BsmAI 628 GAGAC GTCTC(1/5)
BsmFI 81 GTCCC GGGAC(10/14)
BspMI 63 GCAGGT ACCTGC(4/8)
BstEII 375 GGTCACC G^GT[ACGT]ACC
BstPI 375 GGTCACC G^GT[ACGT]ACC
BstXI 533 CCATCTGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 301 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 424 CGGCCG C^GGCCG
EclXI 424 CGGCCG C^GGCCG
Eco52I 424 CGGCCG C^GGCCG
EcoO65I 375 GGTCACC G^GT[ACGT]ACC
FauI 148 GCGGG CCCGC(4/6)
FspI 317 TGCGCA TGC^GCA
HincII 168 GTCAAC GT[CT]^[GA]AC
HindII 168 GTCAAC GT[CT]^[GA]AC
KspI 91 CCGCGG CCGC^GG
MaeI 202 CTAG C^TAG
MboII 587 TCTTC GAAGA(8/7)
MslI 534 CATCTGTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 617 GCCGGC GCC^GGC
NgoMIV 617 GCCGGC G^CCGGC
NheI 201 GCTAGC G^CTAGC
NspI 50 ACATGC [GA]CATG^[CT]
PflMI 300 CCACCATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 602 GACAGTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 253 CAGCTG CAG^CTG
SacI 261 GAGCTC GAGCT^C
SacII 91 CCGCGG CCGC^GG
SmlI 17 CTCAAG C^T[CT][GA]AG
Van91I 300 CCACCATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccII 92 CGCG CG^CG
147 CGCG
AcyI 337 GACGCC G[GA]^CG[CT]C
620 GGCGTC
AspHI 217 GTGCTC G[AT]GC[AT]^C
261 GAGCTC
BalI 542 TGGCCA TGG^CCA
564 TGGCCA
BanII 261 GAGCTC G[GA]GC[CT]^C
471 GGGCTC
BglI 394 GCCGGGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
566 GCCATGGCGGC
BsaHI 337 GACGCC G[GA]^CG[CT]C
620 GGCGTC
BsiHKAI 217 GTGCTC G[AT]GC[AT]^C
261 GAGCTC
BsrBI 183 CCGCTC CCGCTC(-3/-3)
516 GAGCGG
BsrDI 453 CATTGC GCAATG(2/0)
642 CATTGC
BsrFI 379 ACCGGC [GA]^CCGG[CT]
617 GCCGGC
BstF5I 288 CATCC GGATG(2/0)
635 CATCC
BstUI 92 CGCG CG^CG
147 CGCG
Cfr10I 379 ACCGGC [GA]^CCGG[CT]
617 GCCGGC
FokI 288 CATCC GGATG(9/13)
635 CATCC
HaeII 95 GGCGCT [GA]GCGC^[CT]
102 GGCGCT
HgaI 337 GACGC GACGC(5/10)
621 GCGTC
Hin1I 337 GACGCC G[GA]^CG[CT]C
620 GGCGTC
MaeIII 376 GTCAC ^GT[ACGT]AC
623 GTCAC
MluNI 542 TGGCCA TGG^CCA
564 TGGCCA
MscI 542 TGGCCA TGG^CCA
564 TGGCCA
MvnI 92 CGCG CG^CG
147 CGCG
NcoI 567 CCATGG C^CATGG
576 CCATGG
SfiI 393 GGCCGGGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
565 GGCCATGGCGGCC
Tsp45I 376 GTCAC ^GT[GC]AC
623 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AciI AluI BbvI BcnI BmyI
BsaJI BsiYI BslI Bsp1286I BssKI
BssSI BstNI Cac8I CfoI Cfr13I
DdeI DsaI EaeI EcoRII EcoT14I
Fnu4HI HaeIII HapII HhaI HinP1I
HpaII HphI ItaI MnlI MspA1I
MspI MvaI MwoI NciI NlaIII
NlaIV Sau96I ScrFI SfaNI StyI
TseI TspRI XcmI