Restriction map for
pF1KB7102
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# NO CUT ENZYMES ( 150 enzymes) #
##################################
Acc65I AccIII AclI AcsI AfaI
AflII AflIII AgeI Alw44I Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspI AviII AvrII
BamHI BanI BbeI BbrPI BbsI
BcgI BclI BfaI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaI BsaWI BseAI
BseRI BsgI BsiEI BsiWI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstZ17I CelII
CfoI ClaI CpoI DraI DraIII
DrdI DsaI EagI EarI EclXI
Eco47III Eco52I EcoNI EcoRI EcoRV
EcoT22I FbaI FseI FspI HaeII
HhaI HinP1I HindIII HpaI HphI
KasI KpnI Ksp632I KspI MaeI
MamI MfeI MluI MroI MslI
MspA1I MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsaI RsrII SacII SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TfiI Tsp45I Tsp509I Tth111I
Van91I XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AatII 487 GACGTC GACGT^C
AccI 209 GTCGAC GT^[AC][GT]AC
AcyI 487 GACGTC G[GA]^CG[CT]C
AhdI 335 GACTTCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 449 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 312 CCTCAGG CC^T[ACGT]AGG
AspEI 335 GACTTCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 408 TGGCCA TGG^CCA
BbvCI 454 GCTGAGG CCTCAGC(-5/-2)
BciVI 576 GGATAC GTATCC(6/5)
BsaHI 487 GACGTC G[GA]^CG[CT]C
BsmBI 166 CGTCTC CGTCTC(1/5)
BsrDI 259 CATTGC GCAATG(2/0)
BsrFI 480 GCCGGT [GA]^CCGG[CT]
BsrI 156 ACTGG ACTGG(1/-1)
BstEII 6 GGTTACC G^GT[ACGT]ACC
BstF5I 442 CATCC GGATG(2/0)
BstPI 6 GGTTACC G^GT[ACGT]ACC
BstXI 321 CCAGCTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 312 CCTCAGG CC^T[ACGT]AGG
Cfr10I 480 GCCGGT [GA]^CCGG[CT]
EaeI 408 TGGCCA [CT]^GGCC[GA]
Eam1105I 335 GACTTCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 312 CCTCAGG CC^T[ACGT]AGG
EcoO65I 6 GGTTACC G^GT[ACGT]ACC
EcoT14I 432 CCTTGG C^C[AT][AT]GG
FauI 162 CCCGC CCCGC(4/6)
FokI 442 CATCC GGATG(9/13)
Hin1I 487 GACGTC G[GA]^CG[CT]C
HinfI 602 GAGTC G^A[ACGT]TC
MaeII 488 ACGT A^CGT
MaeIII 7 GTTAC ^GT[ACGT]AC
MluNI 408 TGGCCA TGG^CCA
MscI 408 TGGCCA TGG^CCA
MseI 112 TTAA T^TAA
MwoI 152 GCTCACTGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NspI 501 GCATGT [GA]CATG^[CT]
PleI 602 GAGTC GAGTC(4/5)
PpuMI 515 AGGTCCT [GA]G^G[AT]CC[CT]
PstI 496 CTGCAG CTGCA^G
SacI 121 GAGCTC GAGCT^C
SalI 209 GTCGAC G^TCGAC
SfcI 496 CTGCAG C^T[GA][CT]AG
SmaI 381 CCCGGG CCC^GGG
StyI 432 CCTTGG C^C[AT][AT]GG
Tru9I 112 TTAA T^TAA
XmaCI 381 CCCGGG C^CCGGG
XmaI 381 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccII 164 CGCG CG^CG
561 CGCG
ApaI 159 GGGCCC GGGCC^C
187 GGGCCC
AspHI 121 GAGCTC G[AT]GC[AT]^C
565 GTGCTC
AvaI 271 CTCGGG C^[CT]CG[GA]G
381 CCCGGG
AvaII 361 GGTCC G^G[AT]CC
516 GGTCC
BcnI 381 CCCGG CC^[GC]GG
382 CCGGG
BpmI 319 CTCCAG CTGGAG(16/14)
171 CTGGAG
BsiHKAI 121 GAGCTC G[AT]GC[AT]^C
565 GTGCTC
BsiYI 350 CCAGGTTCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
427 CCGTGCCTTGG
BslI 350 CCAGGTTCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
427 CCGTGCCTTGG
BsmFI 60 GGGAC GGGAC(10/14)
266 GTCCC
BsoBI 271 CTCGGG C^[CT]CG[GA]G
381 CCCGGG
BstUI 164 CGCG CG^CG
561 CGCG
Cac8I 161 GCCCGC GC[ACGT]^[ACGT]GC
216 GCTTGC
HapII 382 CCGG C^CGG
481 CCGG
HgaI 559 GACGC GACGC(5/10)
165 GCGTC
HpaII 382 CCGG C^CGG
481 CCGG
MspI 382 CCGG C^CGG
481 CCGG
MvnI 164 CGCG CG^CG
561 CGCG
NciI 381 CCCGG CC^[GC]GG
382 CCGGG
SfaNI 441 GCATC GCATC(5/9)
585 GCATC
TaqI 210 TCGA T^CGA
467 TCGA
TspRI 155 CACTG CA[GC]TG[ACGT][ACGT]^
449 CAGTG
TthHB8I 210 TCGA T^CGA
467 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AciI AluI AlwI BanII BbvI
BmyI BsaJI BsmAI Bsp1286I BssKI
BstNI BstYI Cfr13I DdeI DpnII
DraII EcoO109I EcoRII Fnu4HI HaeIII
HincII HindII ItaI MboI MboII
MflI MnlI MvaI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI TseI
XhoII