Restriction map for
pF1KB7101
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# NO CUT ENZYMES ( 154 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AspHI AspI AviII BalI
BbeI BbrPI BbvCI BcgI BciVI
BclI BfrI BglII BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsiEI BsiHKAI BsiWI BsmFI
Bsp1407I BspEI BspHI BspLU11I BspMI
BsrDI BsrFI BsrGI BsrI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I Bsu36I CelII
Cfr10I CpoI DraI DraIII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FauI FbaI
FseI FspI Hin1I HincII HindII
HindIII HpaI KasI KpnI KspI
MaeII MamI MfeI MluI MluNI
MroI MscI MseI MslI MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tru9I Tsp509I Tth111I
Van91I XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccII 295 CGCG CG^CG
AluI 57 AGCT AG^CT
AlwNI 607 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 440 GGGCCC GGGCC^C
AvaI 517 CCCGGG C^[CT]CG[GA]G
AvrII 501 CCTAGG C^CTAGG
BanI 505 GGCACC G^G[CT][GA]CC
BbsI 210 GAAGAC GAAGAC(2/6)
BfaI 502 CTAG C^TAG
BglI 479 GCCTCAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 501 CCTAGG C^CTAGG
BpuAI 210 GAAGAC GAAGAC(2/6)
BsgI 472 GTGCAG GTGCAG(16/14)
BsmBI 463 GAGACG CGTCTC(1/5)
BsmI 265 GCATTC GAATGC(1/-1)
BsoBI 517 CCCGGG C^[CT]CG[GA]G
BspDI 365 ATCGAT AT^CGAT
BsrBI 223 GAGCGG CCGCTC(-3/-3)
BstF5I 448 CATCC GGATG(2/0)
BstUI 295 CGCG CG^CG
ClaI 365 ATCGAT AT^CGAT
EarI 220 GAAGAG CTCTTC(1/4)
EcoT14I 501 CCTAGG C^C[AT][AT]GG
FokI 448 CATCC GGATG(9/13)
HgaI 465 GACGC GACGC(5/10)
HphI 349 GGTGA GGTGA(8/7)
Ksp632I 220 GAAGAG CTCTTC(1/4)
MaeI 502 CTAG C^TAG
MvnI 295 CGCG CG^CG
NspI 276 GCATGT [GA]CATG^[CT]
PleI 492 GACTC GAGTC(4/5)
PshAI 352 GACAGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 59 CTGCAG CTGCA^G
PvuII 56 CAGCTG CAG^CTG
SapI 220 GAAGAGC GCTCTTC(1/4)
SfaNI 28 GCATC GCATC(5/9)
SfcI 59 CTGCAG C^T[GA][CT]AG
SmaI 517 CCCGGG CCC^GGG
SmlI 616 CTCAAG C^T[CT][GA]AG
StyI 501 CCTAGG C^C[AT][AT]GG
Tsp45I 350 GTGAC ^GT[GC]AC
XmaCI 517 CCCGGG C^CCGGG
XmaI 517 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AfaI 102 GTAC GT^AC
198 GTAC
Asp700I 261 GAAAGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
363 GAATCGATTC
AvaII 79 GGTCC G^G[AT]CC
145 GGTCC
BamHI 3 GGATCC G^GATCC
419 GGATCC
BanII 138 GAGCCC G[GA]GC[CT]^C
440 GGGCCC
BmyI 138 GAGCCC G[AGT]GC[ACT]^C
440 GGGCCC
Bsp1286I 138 GAGCCC G[AGT]GC[ACT]^C
440 GGGCCC
BstYI 3 GGATCC [GA]^GATC[CT]
419 GGATCC
Cac8I 191 GCCAGC GC[ACGT]^[ACGT]GC
569 GCCAGC
CfoI 85 GCGC GCG^C
567 GCGC
HaeII 84 AGCGCC [GA]GCGC^[CT]
566 AGCGCC
HapII 143 CCGG C^CGG
518 CCGG
HhaI 85 GCGC GCG^C
567 GCGC
HinP1I 85 GCGC G^CGC
567 GCGC
HpaII 143 CCGG C^CGG
518 CCGG
MflI 3 GGATCC [GA]^GATC[CT]
419 GGATCC
MspA1I 56 CAGCTG C[AC]G^C[GT]G
193 CAGCGG
MspI 143 CCGG C^CGG
518 CCGG
NlaIII 277 CATG CATG^
303 CATG
RsaI 102 GTAC GT^AC
198 GTAC
TaqI 366 TCGA T^CGA
384 TCGA
TspRI 483 CAGTG CA[GC]TG[ACGT][ACGT]^
622 CAGTG
TthHB8I 366 TCGA T^CGA
384 TCGA
XhoII 3 GGATCC [GA]^GATC[CT]
419 GGATCC
XmnI 261 GAAAGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
363 GAATCGATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AciI AlwI BbvI BcnI BpmI
BsaJI BseRI BsiYI BslI BsmAI
BssKI BstNI Cfr13I DdeI DpnII
DraII EcoO109I EcoRII Fnu4HI HaeIII
HinfI ItaI MaeIII MboI MboII
MnlI MvaI MwoI NciI NdeII
NlaIV Sau3AI Sau96I ScrFI TfiI
TseI