Restriction map for
pF1KB7097
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# NO CUT ENZYMES ( 174 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AgeI
AhdI AluI Alw44I AlwNI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BanII BbeI
BbrPI BbsI BbvCI BciVI BclI
BcnI BfaI BfrI BglI BglII
BlnI BlpI BpmI Bpu1102I BpuAI
BsaBI BsaHI BsaJI BsaWI BseAI
BsgI BsiWI BsmBI BsmI BsoBI
BspDI BspEI BsrBI BsrDI BsrFI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HapII HgaI Hin1I HincII
HindII HindIII HpaI HpaII HphI
KasI KpnI KspI MaeI MaeIII
MamI MfeI MluI MroI MseI
MspA1I MspI MunI MvnI MwoI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuII RsrII
SacI SacII SalI SapI SbfI
ScaI SfcI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspI StyI SwaI
TaqI TfiI Tru9I Tsp45I Tsp509I
Tth111I TthHB8I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AflIII 97 ACATGT A^C[GA][CT]GT
AspHI 557 GAGCAC G[AT]GC[AT]^C
BamHI 329 GGATCC G^GATCC
BanI 544 GGTGCC G^G[CT][GA]CC
BcgI 407 GCAGCGCCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaAI 16 TACGTG [CT]AC^GT[GA]
BsaI 294 GGTCTC GGTCTC(1/5)
BsiEI 9 CGATCG CG[GA][CT]^CG
BsiHKAI 557 GAGCAC G[AT]GC[AT]^C
BsmAI 295 GTCTC GTCTC(1/5)
Bsp1407I 422 TGTACA T^GTACA
BspHI 53 TCATGA T^CATGA
BspLU11I 97 ACATGT A^CATGT
BspMI 367 ACCTGC ACCTGC(4/8)
BsrGI 422 TGTACA T^GTACA
BsrI 333 CCAGT ACTGG(1/-1)
BssSI 161 CTCGTG CACGAG(-5/-1)
BstYI 329 GGATCC [GA]^GATC[CT]
CfoI 410 GCGC GCG^C
DraII 464 AGGTCCT [GA]G^G[ACGT]CC[CT]
EarI 397 CTCTTC CTCTTC(1/4)
EcoO109I 464 AGGTCCT [GA]G^G[ACGT]CC[CT]
FauI 440 CCCGC CCCGC(4/6)
HaeII 409 AGCGCC [GA]GCGC^[CT]
HhaI 410 GCGC GCG^C
HinP1I 410 GCGC G^CGC
HinfI 395 GACTC G^A[ACGT]TC
Ksp632I 397 CTCTTC CTCTTC(1/4)
MaeII 17 ACGT A^CGT
MflI 329 GGATCC [GA]^GATC[CT]
PleI 395 GACTC GAGTC(4/5)
PpuMI 464 AGGTCCT [GA]G^G[AT]CC[CT]
PvuI 9 CGATCG CGAT^CG
RcaI 53 TCATGA T^CATGA
SexAI 233 ACCAGGT A^CC[AT]GGT
SspBI 422 TGTACA T^GTACA
StuI 356 AGGCCT AGG^CCT
TspRI 560 CACTG CA[GC]TG[ACGT][ACGT]^
XhoII 329 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AfaI 15 GTAC GT^AC
423 GTAC
AlwI 330 GATCC GGATC(4/5)
329 GGATC
BalI 20 TGGCCA TGG^CCA
80 TGGCCA
BmyI 545 GTGCCC G[AGT]GC[ACT]^C
557 GAGCAC
Bsp1286I 545 GTGCCC G[AGT]GC[ACT]^C
557 GAGCAC
Cac8I 207 GCTGGC GC[ACGT]^[ACGT]GC
253 GCCTGC
DpnII 10 GATC ^GATC
330 GATC
MboI 10 GATC ^GATC
330 GATC
MboII 179 TCTTC GAAGA(8/7)
398 TCTTC
MluNI 20 TGGCCA TGG^CCA
80 TGGCCA
MscI 20 TGGCCA TGG^CCA
80 TGGCCA
NdeII 10 GATC ^GATC
330 GATC
NspI 97 ACATGT [GA]CATG^[CT]
257 GCATGT
RsaI 15 GTAC GT^AC
423 GTAC
Sau3AI 10 GATC ^GATC
330 GATC
SfaNI 498 GATGC GCATC(5/9)
71 GCATC
#########################################
# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AciI AocI AvaII BbvI BseRI
BsiYI BslI BsmFI BssKI BstF5I
BstNI Bsu36I Cfr13I DdeI EaeI
Eco81I EcoRII Fnu4HI FokI HaeIII
ItaI MnlI MslI MvaI NlaIII
NlaIV Sau96I ScrFI TseI