Restriction map for
pF1KB7096
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# NO CUT ENZYMES ( 166 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaII AviII
AvrII BalI BamHI BbrPI BbsI
BbvCI BcgI BclI BfrI BglI
BglII BlnI BpuAI BsaAI BsaBI
BsaI BsaWI BseAI BseRI BsgI
BsiEI BsiWI BsmAI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
Cfr10I ClaI CpoI DraI DraII
DraIII EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HgaI HincII HindII HindIII
HpaI HphI KpnI KspI MaeII
MamI MfeI MluI MluNI MroI
MscI MseI MslI MunI NaeI
NcoI NdeI NgoMIV NheI NlaIII
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI SexAI SfaNI SfcI SfiI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TaqI Tru9I Tsp509I Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XmnI
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# ONE CUT ENZYMES ( 28 enzymes) #
###################################
AccI 199 GTCTAC GT^[AC][GT]AC
AlwI 557 GGATC GGATC(4/5)
AspHI 234 GAGCAC G[AT]GC[AT]^C
AvaI 477 CCCGGG C^[CT]CG[GA]G
BanII 61 GAGCCC G[GA]GC[CT]^C
BciVI 120 GTATCC GTATCC(6/5)
BfaI 130 CTAG C^TAG
BlpI 568 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 568 GCTAAGC GC^T[ACGT]AGC
BsiHKAI 234 GAGCAC G[AT]GC[AT]^C
BsoBI 477 CCCGGG C^[CT]CG[GA]G
BstYI 557 GGATCT [GA]^GATC[CT]
CelII 568 GCTAAGC GC^T[ACGT]AGC
Cfr13I 100 GGGCC G^G[ACGT]CC
DrdI 403 GACACCGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 277 TGGCCG [CT]^GGCC[GA]
EcoT14I 239 CCTTGG C^C[AT][AT]GG
MaeI 130 CTAG C^TAG
MaeIII 416 GTGAC ^GT[ACGT]AC
MflI 557 GGATCT [GA]^GATC[CT]
PvuII 150 CAGCTG CAG^CTG
Sau96I 100 GGGCC G^G[ACGT]CC
SmaI 477 CCCGGG CCC^GGG
StyI 239 CCTTGG C^C[AT][AT]GG
Tsp45I 416 GTGAC ^GT[GC]AC
XhoII 557 GGATCT [GA]^GATC[CT]
XmaCI 477 CCCGGG C^CCGGG
XmaI 477 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccII 511 CGCG CG^CG
525 CGCG
AcyI 54 GGCGCC G[GA]^CG[CT]C
467 GGCGCC
BbeI 54 GGCGCC GGCGC^C
467 GGCGCC
BmyI 61 GAGCCC G[AGT]GC[ACT]^C
234 GAGCAC
BsaHI 54 GGCGCC G[GA]^CG[CT]C
467 GGCGCC
Bsp1286I 61 GAGCCC G[AGT]GC[ACT]^C
234 GAGCAC
BstF5I 382 CATCC GGATG(2/0)
445 CATCC
BstUI 511 CGCG CG^CG
525 CGCG
CfoI 55 GCGC GCG^C
468 GCGC
DpnII 94 GATC ^GATC
558 GATC
DsaI 103 CCGTGG C^C[GA][CT]GG
463 CCGTGG
EarI 110 CTCTTC CTCTTC(1/4)
313 GAAGAG
FauI 30 GCGGG CCCGC(4/6)
422 GCGGG
FokI 382 CATCC GGATG(9/13)
445 CATCC
HaeII 54 GGCGCC [GA]GCGC^[CT]
467 GGCGCC
HhaI 55 GCGC GCG^C
468 GCGC
Hin1I 54 GGCGCC G[GA]^CG[CT]C
467 GGCGCC
HinP1I 55 GCGC G^CGC
468 GCGC
KasI 54 GGCGCC G^GCGCC
467 GGCGCC
Ksp632I 110 CTCTTC CTCTTC(1/4)
313 GAAGAG
MboI 94 GATC ^GATC
558 GATC
MspA1I 150 CAGCTG C[AC]G^C[GT]G
420 CAGCGG
MvnI 511 CGCG CG^CG
525 CGCG
MwoI 46 GCAGAGGTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
351 GCTCCAGCAGC
NarI 54 GGCGCC GG^CGCC
467 GGCGCC
NdeII 94 GATC ^GATC
558 GATC
Sau3AI 94 GATC ^GATC
558 GATC
ScaI 126 AGTACT AGT^ACT
137 AGTACT
SfoI 54 GGCGCC GGC^GCC
467 GGCGCC
TfiI 40 GATTC G^A[AT]TC
504 GATTC
TspRI 143 CACTG CA[GC]TG[ACGT][ACGT]^
264 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AciI AfaI AluI BanI BbvI
BcnI BpmI BsaJI BsiYI BslI
BsmFI BsrI BssKI BstNI Cac8I
DdeI EcoRII Fnu4HI HaeIII HapII
HinfI HpaII ItaI MboII MnlI
MspI MvaI NciI NlaIV PleI
RsaI ScrFI TseI