Restriction map for
pF1KB7095
##################################
# NO CUT ENZYMES ( 160 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcsI AflII AgeI AhdI AlwNI
AocI Aor51HI ApaI ApoI AscI
AseI AsnI Asp700I AspEI AvaI
AviII AvrII BalI BamHI BanII
BbeI BbsI BbvCI BcgI BciVI
BclI BcnI BfrI BglI BglII
BlnI BpmI BpuAI BsaBI BsaI
BsaWI BseAI BseRI BsiEI BsiWI
BsmI BsoBI Bsp1407I BspDI BspEI
BspLU11I BsrGI BssHII BssKI Bst1107I
BstAPI BstBI BstEII BstNI BstPI
BstUI BstYI BstZ17I Bsu36I CfoI
ClaI CpoI DraI DraII DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRII EcoT22I FauI FbaI
FseI FspI HaeII HhaI HinP1I
HincII HindII HindIII HpaI KasI
Ksp632I KspI MamI MboII MflI
MluI MluNI MroI MscI MseI
MvaI MvnI MwoI NarI NciI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmeI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RsrII SacI SacII SalI
SapI SbfI ScaI ScrFI SexAI
SfaNI SfcI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TaqI Tru9I TthHB8I XbaI
XhoI XhoII XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 67 enzymes) #
###################################
Acc65I 204 GGTACC G^GTACC
AcyI 385 GACGCC G[GA]^CG[CT]C
AflIII 392 ACACGT A^C[GA][CT]GT
Alw44I 490 GTGCAC G^TGCAC
AlwI 519 GATCC GGATC(4/5)
ApaLI 490 GTGCAC G^TGCAC
Asp718I 204 GGTACC G^GTACC
AspHI 490 GTGCAC G[AT]GC[AT]^C
AspI 399 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 398 GGACC G^G[AT]CC
BanI 204 GGTACC G^G[CT][GA]CC
BbrPI 393 CACGTG CAC^GTG
BfaI 125 CTAG C^TAG
BlpI 329 GCTCAGC GC^T[ACGT]AGC
BmyI 490 GTGCAC G[AGT]GC[ACT]^C
Bpu1102I 329 GCTCAGC GC^T[ACGT]AGC
BsaAI 393 CACGTG [CT]AC^GT[GA]
BsaHI 385 GACGCC G[GA]^CG[CT]C
BsaJI 140 CCATGG C^C[ACGT][ACGT]GG
BsgI 304 CTGCAC GTGCAG(16/14)
BsiHKAI 490 GTGCAC G[AT]GC[AT]^C
BsmBI 232 CGTCTC CGTCTC(1/5)
BsmFI 375 GTCCC GGGAC(10/14)
Bsp1286I 490 GTGCAC G[AGT]GC[ACT]^C
BspHI 356 TCATGA T^CATGA
BspMI 201 GCAGGT ACCTGC(4/8)
BsrBI 327 CCGCTC CCGCTC(-3/-3)
BsrDI 89 GCAATG GCAATG(2/0)
BsrFI 410 GCCGGC [GA]^CCGG[CT]
BssSI 12 CTCGTG CACGAG(-5/-1)
BstXI 468 CCAATTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 410 GCCGGC GC[ACGT]^[ACGT]GC
CelII 329 GCTCAGC GC^T[ACGT]AGC
Cfr10I 410 GCCGGC [GA]^CCGG[CT]
DraIII 271 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 140 CCATGG C^C[GA][CT]GG
EaeI 98 TGGCCG [CT]^GGCC[GA]
EcoRV 187 GATATC GAT^ATC
EcoT14I 140 CCATGG C^C[AT][AT]GG
HgaI 385 GACGC GACGC(5/10)
Hin1I 385 GACGCC G[GA]^CG[CT]C
HphI 137 TCACC GGTGA(8/7)
KpnI 204 GGTACC GGTAC^C
MaeI 125 CTAG C^TAG
MaeIII 239 GTGAC ^GT[ACGT]AC
MfeI 469 CAATTG C^AATTG
MslI 8 CACCCTCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 469 CAATTG C^AATTG
NaeI 410 GCCGGC GCC^GGC
NcoI 140 CCATGG C^CATGG
NgoMIV 410 GCCGGC G^CCGGC
NlaIV 204 GGTACC GG[ACGT]^[ACGT]CC
NspI 197 ACATGC [GA]CATG^[CT]
PflFI 399 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 270 CCACAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 217 GACTC GAGTC(4/5)
PmaCI 393 CACGTG CAC^GTG
PmlI 393 CACGTG CAC^GTG
PvuII 332 CAGCTG CAG^CTG
RcaI 356 TCATGA T^CATGA
StyI 140 CCATGG C^C[AT][AT]GG
TfiI 555 GATTC G^A[AT]TC
Tsp45I 239 GTGAC ^GT[GC]AC
Tsp509I 470 AATT ^AATT
Tth111I 399 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 270 CCACAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 371 CCATGTCCCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 12 enzymes) #
###################################
BsmAI 154 GTCTC GTCTC(1/5)
233 GTCTC
BstF5I 420 CATCC GGATG(2/0)
453 CATCC
DpnII 135 GATC ^GATC
519 GATC
FokI 420 CATCC GGATG(9/13)
453 CATCC
HapII 36 CCGG C^CGG
411 CCGG
HinfI 217 GACTC G^A[ACGT]TC
555 GATTC
HpaII 36 CCGG C^CGG
411 CCGG
MaeII 231 ACGT A^CGT
394 ACGT
MboI 135 GATC ^GATC
519 GATC
MspI 36 CCGG C^CGG
411 CCGG
NdeII 135 GATC ^GATC
519 GATC
Sau3AI 135 GATC ^GATC
519 GATC
#########################################
# 3 OR MORE CUT ENZYMES ( 19 enzymes) #
#########################################
AciI AfaI AluI BbvI BsiYI
BslI BsrI Cfr13I DdeI Fnu4HI
HaeIII ItaI MnlI MspA1I NlaIII
RsaI Sau96I TseI TspRI