##################################
# NO CUT ENZYMES ( 142 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvrII BamHI
BanI BbeI BbsI BbvCI BcgI
BciVI BclI BfrI BglII BlnI
BlpI BpmI Bpu1102I BpuAI BsaBI
BsaI BsaWI BseAI BsiHKAI BsiWI
BsmAI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrGI
BsrI BssSI Bst1107I BstBI BstEII
BstPI BstZ17I Bsu36I CelII ClaI
CpoI DraI Eam1105I EarI Eco47III
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI HincII HindII HindIII
HinfI HpaI KasI KpnI Ksp632I
KspI MaeIII MamI MfeI MluI
MroI MseI MslI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PflFI PinAI PleI PmeI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TfiI Tru9I
Tsp45I Tsp509I Tth111I XbaI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 59 enzymes) #
###################################
AcyI 63 GGCGTC G[GA]^CG[CT]C
AfaI 126 GTAC GT^AC
AflIII 434 ACGTGT A^C[GA][CT]GT
AlwI 515 GGATC GGATC(4/5)
AlwNI 513 CAGGATCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaI 361 CTCGAG C^[CT]CG[GA]G
AviII 509 TGCGCA TGC^GCA
BalI 290 TGGCCA TGG^CCA
BanII 490 GAGCCC G[GA]GC[CT]^C
BbrPI 433 CACGTG CAC^GTG
BfaI 448 CTAG C^TAG
BglI 292 GCCATGACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 490 GAGCCC G[AGT]GC[ACT]^C
BsaAI 433 CACGTG [CT]AC^GT[GA]
BsaHI 63 GGCGTC G[GA]^CG[CT]C
BseRI 244 GAGGAG GAGGAG(10/8)
BsgI 379 CTGCAC GTGCAG(16/14)
BsoBI 361 CTCGAG C^[CT]CG[GA]G
Bsp1286I 490 GAGCCC G[AGT]GC[ACT]^C
BsrBI 498 CCGCTC CCGCTC(-3/-3)
BsrFI 335 ACCGGC [GA]^CCGG[CT]
BssHII 280 GCGCGC G^CGCGC
BstAPI 512 GCAGGATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 515 GGATCT [GA]^GATC[CT]
Cfr10I 335 ACCGGC [GA]^CCGG[CT]
DdeI 52 CTGAG C^T[ACGT]AG
DpnII 516 GATC ^GATC
DraIII 313 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 405 GACCGCCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 186 CCTGGTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 68 CCAAGG C^C[AT][AT]GG
FauI 484 CCCGC CCCGC(4/6)
FspI 509 TGCGCA TGC^GCA
HgaI 64 GCGTC GACGC(5/10)
Hin1I 63 GGCGTC G[GA]^CG[CT]C
MaeI 448 CTAG C^TAG
MaeII 434 ACGT A^CGT
MboI 516 GATC ^GATC
MflI 515 GGATCT [GA]^GATC[CT]
MluNI 290 TGGCCA TGG^CCA
MscI 290 TGGCCA TGG^CCA
MspA1I 232 CAGCTG C[AC]G^C[GT]G
NdeII 516 GATC ^GATC
NlaIII 294 CATG CATG^
PaeR7I 361 CTCGAG C^TCGAG
PmaCI 433 CACGTG CAC^GTG
PmlI 433 CACGTG CAC^GTG
PpuMI 393 GGGTCCC [GA]G^G[AT]CC[CT]
PshAI 297 GACGGCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 31 CTGCAG CTGCA^G
PvuII 232 CAGCTG CAG^CTG
RsaI 126 GTAC GT^AC
Sau3AI 516 GATC ^GATC
SfcI 31 CTGCAG C^T[GA][CT]AG
SfiI 291 GGCCATGACGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
StyI 68 CCAAGG C^C[AT][AT]GG
XcmI 68 CCAAGGCAGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 361 CTCGAG C^TCGAG
XhoII 515 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AvaII 228 GGACC G^G[AT]CC
394 GGTCC
BcnI 276 CCGGG CC^[GC]GG
353 CCGGG
BspMI 374 ACCTGC ACCTGC(4/8)
506 ACCTGC
BstXI 17 CCAAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
68 CCAAGGCAGTGG
DraII 393 GGGTCCC [GA]G^G[ACGT]CC[CT]
524 AGGGCCT
DsaI 389 CCGTGG C^C[GA][CT]GG
417 CCACGG
EagI 299 CGGCCG C^GGCCG
420 CGGCCG
EclXI 299 CGGCCG C^GGCCG
420 CGGCCG
Eco52I 299 CGGCCG C^GGCCG
420 CGGCCG
EcoO109I 393 GGGTCCC [GA]G^G[ACGT]CC[CT]
524 AGGGCCT
HaeII 252 GGCGCT [GA]GCGC^[CT]
263 GGCGCT
HphI 241 GGTGA GGTGA(8/7)
326 TCACC
MboII 42 GAAGA GAAGA(8/7)
171 GAAGA
NciI 276 CCGGG CC^[GC]GG
353 CCGGG
PflMI 312 CCACCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
384 CCAGGCCGTGG
SexAI 185 ACCTGGT A^CC[AT]GGT
314 ACCAGGT
SmlI 178 CTCAAG C^T[CT][GA]AG
361 CTCGAG
TaqI 332 TCGA T^CGA
362 TCGA
TspRI 74 CAGTG CA[GC]TG[ACGT][ACGT]^
105 CAGTG
TthHB8I 332 TCGA T^CGA
362 TCGA
Van91I 312 CCACCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
384 CCAGGCCGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AccII AciI AluI BbvI BsaJI
BsiEI BsiYI BslI BsmFI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I EaeI EcoRII Fnu4HI FokI
HaeIII HapII HhaI HinP1I HpaII
ItaI MnlI MspI MvaI MvnI
MwoI NlaIV Sau96I ScrFI SfaNI
TseI