Restriction map for
pF1KB7092
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# NO CUT ENZYMES ( 150 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AgeI AhdI Alw44I AlwI
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AvaI AvaII AvrII BalI BamHI
BbsI BbvCI BcgI BciVI BclI
BcnI BglI BglII BlnI BpuAI
BsaBI BsaI BsaWI BseAI BsmAI
BsmBI BsmFI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrGI BssKI Bst1107I BstBI
BstF5I BstNI BstXI BstYI BstZ17I
Bsu36I ClaI CpoI DraI DraIII
DrdI Eam1105I EarI Eco47III Eco81I
EcoNI EcoRI EcoRII EcoRV EcoT14I
EcoT22I FbaI FokI FseI HgaI
HincII HindII HpaI KpnI Ksp632I
KspI MamI MboII MfeI MflI
MluNI MroI MscI MunI MvaI
NaeI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflMI PinAI
PmeI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI SbfI ScaI ScrFI SexAI
SfaNI SfiI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI TfiI
Tsp509I TspRI Van91I XbaI XcmI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AcyI 179 GGCGCC G[GA]^CG[CT]C
AflII 13 CTTAAG C^TTAAG
AflIII 408 ACGCGT A^C[GA][CT]GT
AlwNI 28 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 414 GGGCCC GGGCC^C
AviII 353 TGCGCA TGC^GCA
BbeI 179 GGCGCC GGCGC^C
BbrPI 505 CACGTG CAC^GTG
BfaI 511 CTAG C^TAG
BfrI 13 CTTAAG C^TTAAG
BlpI 150 GCTGAGC GC^T[ACGT]AGC
BpmI 283 CTGGAG CTGGAG(16/14)
Bpu1102I 150 GCTGAGC GC^T[ACGT]AGC
BsaHI 179 GGCGCC G[GA]^CG[CT]C
BsaJI 380 CCGTGG C^C[ACGT][ACGT]GG
BsgI 82 CTGCAC GTGCAG(16/14)
BsiEI 227 CGGCCG CG[GA][CT]^CG
BsiWI 405 CGTACG C^GTACG
BsrFI 110 ACCGGC [GA]^CCGG[CT]
BssHII 219 GCGCGC G^CGCGC
CelII 150 GCTGAGC GC^T[ACGT]AGC
Cfr10I 110 ACCGGC [GA]^CCGG[CT]
DdeI 151 CTGAG C^T[ACGT]AG
DraII 414 GGGCCCT [GA]G^G[ACGT]CC[CT]
DsaI 380 CCGTGG C^C[GA][CT]GG
EaeI 227 CGGCCG [CT]^GGCC[GA]
EagI 227 CGGCCG C^GGCCG
EclXI 227 CGGCCG C^GGCCG
Eco52I 227 CGGCCG C^GGCCG
EcoO109I 414 GGGCCCT [GA]G^G[ACGT]CC[CT]
FspI 353 TGCGCA TGC^GCA
HaeII 179 GGCGCC [GA]GCGC^[CT]
HapII 111 CCGG C^CGG
Hin1I 179 GGCGCC G[GA]^CG[CT]C
HindIII 10 AAGCTT A^AGCTT
HinfI 484 GACTC G^A[ACGT]TC
HpaII 111 CCGG C^CGG
HphI 68 TCACC GGTGA(8/7)
KasI 179 GGCGCC G^GCGCC
MaeI 511 CTAG C^TAG
MluI 408 ACGCGT A^CGCGT
MseI 14 TTAA T^TAA
MslI 474 CACTTGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 111 CCGG C^CGG
NarI 179 GGCGCC GG^CGCC
PleI 484 GACTC GAGTC(4/5)
PmaCI 505 CACGTG CAC^GTG
PmlI 505 CACGTG CAC^GTG
PstI 115 CTGCAG CTGCA^G
PvuII 148 CAGCTG CAG^CTG
SacI 286 GAGCTC GAGCT^C
SfcI 115 CTGCAG C^T[GA][CT]AG
SfoI 179 GGCGCC GGC^GCC
TaqI 539 TCGA T^CGA
Tru9I 14 TTAA T^TAA
TthHB8I 539 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AfaI 406 GTAC GT^AC
516 GTAC
AspHI 286 GAGCTC G[AT]GC[AT]^C
340 GTGCTC
AspI 295 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
466 GACTTTGTC
BanI 179 GGCGCC G^G[CT][GA]CC
264 GGTGCC
BanII 286 GAGCTC G[GA]GC[CT]^C
414 GGGCCC
BsaAI 403 CACGTA [CT]AC^GT[GA]
505 CACGTG
BseRI 343 CTCCTC GAGGAG(10/8)
523 GAGGAG
BsiHKAI 286 GAGCTC G[AT]GC[AT]^C
340 GTGCTC
BsrI 59 ACTGG ACTGG(1/-1)
207 CCAGT
BssSI 22 CACGAG CACGAG(-5/-1)
85 CACGAG
BstAPI 27 GCAGAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
84 GCACGAGCTGC
BstEII 66 GGTCACC G^GT[ACGT]ACC
426 GGTTACC
BstPI 66 GGTCACC G^GT[ACGT]ACC
426 GGTTACC
Cfr13I 414 GGGCC G^G[ACGT]CC
415 GGCCC
DpnII 495 GATC ^GATC
541 GATC
EcoO65I 66 GGTCACC G^GT[ACGT]ACC
426 GGTTACC
FauI 436 CCCGC CCCGC(4/6)
123 GCGGG
MboI 495 GATC ^GATC
541 GATC
MspA1I 118 CAGCGG C[AC]G^C[GT]G
148 CAGCTG
NdeII 495 GATC ^GATC
541 GATC
NlaIII 357 CATG CATG^
459 CATG
PflFI 295 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
466 GACTTTGTC
RsaI 406 GTAC GT^AC
516 GTAC
Sau3AI 495 GATC ^GATC
541 GATC
Sau96I 414 GGGCC G^G[ACGT]CC
415 GGCCC
SmlI 13 CTTAAG C^T[CT][GA]AG
346 CTCAAG
Tth111I 295 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
466 GACTTTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 25 enzymes) #
#########################################
AccII AciI AluI BbvI BmyI
BsiYI BslI Bsp1286I BspMI BstUI
Cac8I CfoI Fnu4HI HaeIII HhaI
HinP1I ItaI MaeII MaeIII MnlI
MvnI MwoI NlaIV TseI Tsp45I