Restriction map for
pF1KB7089
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# NO CUT ENZYMES ( 177 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
AlwI AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AvaII AviII AvrII BalI BamHI
BanI BbeI BbrPI BbsI BbvCI
BciVI BclI BcnI BfaI BfrI
BglII BlnI BlpI Bpu1102I BpuAI
BsaBI BsaI BsaWI BseAI BseRI
BsgI BsiWI BsmAI BsmBI BsmFI
BsmI BsoBI Bsp1407I BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BsrI BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstYI BstZ17I
Bsu36I CelII Cfr10I Cfr13I CpoI
DraI DraII DraIII DrdI Eam1105I
Eco47III Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HapII HincII HindII HindIII
HinfI HpaI HpaII KasI KpnI
MaeI MamI MfeI MflI MluI
MluNI MroI MscI MseI MslI
MspI MunI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NlaIII
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacI SalI SapI Sau96I
ScaI SexAI SfaNI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI SspBI SspI StuI SwaI
TfiI Tru9I Tsp509I TspRI Tth111I
Van91I XbaI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AcyI 373 GACGCC G[GA]^CG[CT]C
AflIII 200 ACGTGT A^C[GA][CT]GT
AspHI 88 GTGCTC G[AT]GC[AT]^C
BcgI 321 GCAGGACTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 417 GCCCTCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 420 CTCCAG CTGGAG(16/14)
BsaAI 199 TACGTG [CT]AC^GT[GA]
BsaHI 373 GACGCC G[GA]^CG[CT]C
BsiHKAI 88 GTGCTC G[AT]GC[AT]^C
BspDI 295 ATCGAT AT^CGAT
BstXI 278 CCATCGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 295 ATCGAT AT^CGAT
DdeI 106 CTGAG C^T[ACGT]AG
DpnII 237 GATC ^GATC
EagI 389 CGGCCG C^GGCCG
EarI 58 CTCTTC CTCTTC(1/4)
EclXI 389 CGGCCG C^GGCCG
Eco52I 389 CGGCCG C^GGCCG
EcoT14I 140 CCAAGG C^C[AT][AT]GG
HaeII 288 GGCGCT [GA]GCGC^[CT]
HgaI 373 GACGC GACGC(5/10)
Hin1I 373 GACGCC G[GA]^CG[CT]C
Ksp632I 58 CTCTTC CTCTTC(1/4)
KspI 386 CCGCGG CCGC^GG
MaeII 200 ACGT A^CGT
MaeIII 217 GTCAC ^GT[ACGT]AC
MboI 237 GATC ^GATC
NdeII 237 GATC ^GATC
NlaIV 414 GGAGCC GG[ACGT]^[ACGT]CC
NotI 388 GCGGCCGC GC^GGCCGC
PstI 319 CTGCAG CTGCA^G
SacII 386 CCGCGG CCGC^GG
Sau3AI 237 GATC ^GATC
SbfI 318 CCTGCAGG CCTGCA^GG
SfcI 319 CTGCAG C^T[GA][CT]AG
SmlI 166 CTCAAG C^T[CT][GA]AG
Sse8387I 318 CCTGCAGG CCTGCA^GG
StyI 140 CCAAGG C^C[AT][AT]GG
Tsp45I 217 GTCAC ^GT[GC]AC
XcmI 161 CCATCCTCAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AfaI 198 GTAC GT^AC
204 GTAC
BanII 406 GAGCCC G[GA]GC[CT]^C
415 GAGCCC
BsiEI 99 CGACCG CG[GA][CT]^CG
389 CGGCCG
BssHII 131 GCGCGC G^CGCGC
133 GCGCGC
BssKI 339 CCAGG ^CC[ACGT]GG
422 CCAGG
BstF5I 162 CATCC GGATG(2/0)
428 CATCC
BstNI 339 CCAGG CC^[AT]GG
422 CCAGG
DsaI 377 CCGTGG C^C[GA][CT]GG
386 CCGCGG
EaeI 275 CGGCCA [CT]^GGCC[GA]
389 CGGCCG
EcoRII 339 CCAGG ^CC[AT]GG
422 CCAGG
FokI 162 CATCC GGATG(9/13)
428 CATCC
MspA1I 246 CCGCTG C[AC]G^C[GT]G
386 CCGCGG
MvaI 339 CCAGG CC^[AT]GG
422 CCAGG
RsaI 198 GTAC GT^AC
204 GTAC
ScrFI 339 CCAGG CC^[ACGT]GG
422 CCAGG
TaqI 239 TCGA T^CGA
296 TCGA
TthHB8I 239 TCGA T^CGA
296 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AccII AciI AluI BbvI BmyI
BsaJI BsiYI BslI Bsp1286I BstUI
Cac8I CfoI FauI Fnu4HI HaeIII
HhaI HinP1I HphI ItaI MboII
MnlI MvnI MwoI TseI