Restriction map for
pF1KB7086
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# NO CUT ENZYMES ( 196 enzymes) #
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AatII AccI AccII AccIII AclI
AcsI AcyI AflIII AgeI AhdI
AluI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI AspEI AspHI AspI AvaI
AvaII AviII AvrII BalI BamHI
BanII BbeI BbrPI BbvCI BbvI
BcgI BciVI BcnI BfaI BglII
BlnI BlpI BmyI BpmI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiWI BsmBI BsmFI BsmI BsoBI
Bsp1286I Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstXI BstYI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraI DraII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FauI Fnu4HI
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI HpaII ItaI KasI
KspI MaeI MaeII MamI MfeI
MflI MluI MluNI MroI MscI
MspA1I MspI MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI SexAI
SfaNI SfcI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI StuI SwaI TseI
Tsp45I Tsp509I TspRI Tth111I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 38 enzymes) #
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Acc65I 236 GGTACC G^GTACC
AciI 177 GCGG CCGC(-3/-1)
AflII 256 CTTAAG C^TTAAG
AlwI 208 GGATC GGATC(4/5)
Asp700I 99 GAACTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 236 GGTACC G^GTACC
BanI 236 GGTACC G^G[CT][GA]CC
BbsI 325 GTCTTC GAAGAC(2/6)
BclI 59 TGATCA T^GATCA
BfrI 256 CTTAAG C^TTAAG
BglI 343 GCCTTGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 325 GTCTTC GAAGAC(2/6)
BsiYI 199 CCTCGACATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 199 CCTCGACATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 395 GAGAC GTCTC(1/5)
BstAPI 277 GCACTCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 81 GGATG GGATG(2/0)
Cac8I 348 GCTGGC GC[ACGT]^[ACGT]GC
Cfr13I 341 GGGCC G^G[ACGT]CC
DdeI 17 CTCAG C^T[ACGT]AG
DraIII 278 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 52 CTCTTC CTCTTC(1/4)
FbaI 59 TGATCA T^GATCA
FokI 81 GGATG GGATG(9/13)
HaeIII 342 GGCC GG^CC
HinfI 312 GAATC G^A[ACGT]TC
HphI 32 TCACC GGTGA(8/7)
KpnI 236 GGTACC GGTAC^C
Ksp632I 52 CTCTTC CTCTTC(1/4)
MaeIII 252 GTTAC ^GT[ACGT]AC
Sau96I 341 GGGCC G^G[ACGT]CC
ScaI 24 AGTACT AGT^ACT
SmlI 256 CTTAAG C^T[CT][GA]AG
SspI 217 AATATT AAT^ATT
TaqI 201 TCGA T^CGA
TfiI 312 GAATC G^A[AT]TC
TthHB8I 201 TCGA T^CGA
XmnI 99 GAACTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AfaI 25 GTAC GT^AC
237 GTAC
BsaJI 11 CCTTGG C^C[ACGT][ACGT]GG
94 CCTTGG
BsrDI 243 CATTGC GCAATG(2/0)
247 GCAATG
BssKI 35 CCTGG ^CC[ACGT]GG
233 CCTGG
BstNI 35 CCTGG CC^[AT]GG
233 CCTGG
DpnII 60 GATC ^GATC
209 GATC
EcoRII 35 CCTGG ^CC[AT]GG
233 CCTGG
EcoT14I 11 CCTTGG C^C[AT][AT]GG
94 CCTTGG
MboI 60 GATC ^GATC
209 GATC
MboII 53 TCTTC GAAGA(8/7)
326 TCTTC
MslI 63 CATGTTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
243 CATTGCAATG
MvaI 35 CCTGG CC^[AT]GG
233 CCTGG
MwoI 277 GCACTCTGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
343 GCCTTGCTGGC
NdeII 60 GATC ^GATC
209 GATC
NlaIV 104 GGTTCC GG[ACGT]^[ACGT]CC
236 GGTACC
RsaI 25 GTAC GT^AC
237 GTAC
Sau3AI 60 GATC ^GATC
209 GATC
ScrFI 35 CCTGG CC^[ACGT]GG
233 CCTGG
StyI 11 CCTTGG C^C[AT][AT]GG
94 CCTTGG
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# 3 OR MORE CUT ENZYMES ( 5 enzymes) #
#########################################
BsrI MnlI MseI NlaIII Tru9I