Restriction map for
pF1KB7085
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# NO CUT ENZYMES ( 166 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AflII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BamHI BanI BanII BbeI BbrPI
BbsI BbvCI BciVI BclI BfaI
BfrI BglI BglII BlnI BpuAI
BsaAI BsaBI BsaI BsaWI BseAI
BseRI BsgI BsiEI BsiWI BsmI
Bsp1407I BspDI BspEI BspHI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstYI BstZ17I Bsu36I CfoI Cfr10I
ClaI CpoI DraI DraIII DrdI
DsaI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FseI FspI HaeII HhaI HinP1I
HincII HindII HindIII HpaI KasI
KpnI Ksp632I KspI MaeI MamI
MfeI MflI MluI MroI MseI
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StyI SwaI Tru9I Tsp509I TspRI
Tth111I XbaI XcmI XhoI XhoII
XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AccI 238 GTCTAC GT^[AC][GT]AC
AcyI 27 GACGCC G[GA]^CG[CT]C
AfaI 148 GTAC GT^AC
AflIII 77 ACATGT A^C[GA][CT]GT
AlwI 3 GATCC GGATC(4/5)
AspHI 214 GAGCAC G[AT]GC[AT]^C
AvaI 290 CCCGGG C^[CT]CG[GA]G
BalI 338 TGGCCA TGG^CCA
BbvI 107 GCAGC GCAGC(8/12)
BcgI 246 CGAGTCCGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 186 GCTGAGC GC^T[ACGT]AGC
BmyI 214 GAGCAC G[AGT]GC[ACT]^C
Bpu1102I 186 GCTGAGC GC^T[ACGT]AGC
BsaHI 27 GACGCC G[GA]^CG[CT]C
BsiHKAI 214 GAGCAC G[AT]GC[AT]^C
BsmBI 370 GAGACG CGTCTC(1/5)
BsmFI 99 GTCCC GGGAC(10/14)
BsoBI 290 CCCGGG C^[CT]CG[GA]G
Bsp1286I 214 GAGCAC G[AGT]GC[ACT]^C
BspLU11I 77 ACATGT A^CATGT
BspMI 95 GCAGGT ACCTGC(4/8)
BsrBI 198 CCGCTC CCGCTC(-3/-3)
BsrI 150 ACTGG ACTGG(1/-1)
BstF5I 195 CATCC GGATG(2/0)
BstXI 203 CCAAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 186 GCTGAGC GC^T[ACGT]AGC
DpnII 3 GATC ^GATC
EaeI 338 TGGCCA [CT]^GGCC[GA]
Fnu4HI 107 GCAGC GC^[ACGT]GC
FokI 195 CATCC GGATG(9/13)
HapII 291 CCGG C^CGG
HgaI 27 GACGC GACGC(5/10)
Hin1I 27 GACGCC G[GA]^CG[CT]C
HpaII 291 CCGG C^CGG
ItaI 107 GCAGC GC^[ACGT]GC
MboI 3 GATC ^GATC
MluNI 338 TGGCCA TGG^CCA
MscI 338 TGGCCA TGG^CCA
MslI 18 CACAGCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 291 CCGG C^CGG
NdeII 3 GATC ^GATC
NspI 77 ACATGT [GA]CATG^[CT]
PflMI 41 CCAAGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 247 GAGTC GAGTC(4/5)
RsaI 148 GTAC GT^AC
Sau3AI 3 GATC ^GATC
SfcI 306 CTACAG C^T[GA][CT]AG
SmaI 290 CCCGGG CCC^GGG
StuI 275 AGGCCT AGG^CCT
TfiI 155 GATTC G^A[AT]TC
TseI 107 GCAGC G^C[AT]GC
Van91I 41 CCAAGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 290 CCCGGG C^CCGGG
XmaI 290 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 18 enzymes) #
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AvaII 98 GGTCC G^G[AT]CC
294 GGTCC
BcnI 290 CCCGG CC^[GC]GG
291 CCGGG
BsmAI 55 GAGAC GTCTC(1/5)
370 GAGAC
Cac8I 91 GCCCGC GC[ACGT]^[ACGT]GC
119 GCAAGC
DdeI 187 CTGAG C^T[ACGT]AG
368 CTGAG
FauI 92 CCCGC CCCGC(4/6)
104 CCCGC
HinfI 155 GATTC G^A[ACGT]TC
247 GAGTC
MaeII 137 ACGT A^CGT
236 ACGT
MaeIII 25 GTGAC ^GT[ACGT]AC
113 GTGAC
MboII 158 TCTTC GAAGA(8/7)
167 TCTTC
MspA1I 20 CAGCGG C[AC]G^C[GT]G
108 CAGCGG
NciI 290 CCCGG CC^[GC]GG
291 CCGGG
NlaIV 98 GGTCCC GG[ACGT]^[ACGT]CC
293 GGGTCC
PpuMI 97 AGGTCCC [GA]G^G[AT]CC[CT]
293 GGGTCCT
SfaNI 253 GATGC GCATC(5/9)
179 GCATC
TaqI 220 TCGA T^CGA
245 TCGA
Tsp45I 25 GTGAC ^GT[GC]AC
113 GTGAC
TthHB8I 220 TCGA T^CGA
245 TCGA
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# 3 OR MORE CUT ENZYMES ( 20 enzymes) #
#########################################
AciI AluI BpmI BsaJI BsiYI
BslI BssKI BstNI Cfr13I DraII
EcoO109I EcoRII HaeIII HphI MnlI
MvaI MwoI NlaIII Sau96I ScrFI