##################################
# NO CUT ENZYMES (  196 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AflII       AgeI        
AhdI        Alw44I      AlwNI       AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspHI       AspI        
AvaI        AvaII       AviII       AvrII       BamHI       
BanI        BanII       BbeI        BbrPI       BbvCI       
BbvI        BcgI        BciVI       BclI        BcnI        
BfrI        BglI        BglII       BlnI        BlpI        
Bpu1102I    BsaAI       BsaBI       BsaHI       BsaI        
BsaJI       BsaWI       BseAI       BsiEI       BsiHKAI     
BsiWI       BsmAI       BsmBI       BsmFI       BsmI        
BsoBI       Bsp1407I    BspEI       BspHI       BspMI       
BsrBI       BsrDI       BsrFI       BsrGI       BssHII      
BssKI       BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstNI       BstPI       BstUI       BstXI       
BstYI       BstZ17I     Bsu36I      CelII       CfoI        
Cfr10I      Cfr13I      CpoI        DraII       DraIII      
DrdI        DsaI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRII      EcoRV       EcoT14I     EcoT22I     
FauI        FbaI        Fnu4HI      FseI        FspI        
HaeII       HapII       HgaI        HhaI        Hin1I       
HinP1I      HpaII       HphI        ItaI        KasI        
KpnI        KspI        MaeII       MamI        MfeI        
MflI        MluI        MroI        MspA1I      MspI        
MunI        MvaI        MvnI        MwoI        NaeI        
NarI        NciI        NcoI        NdeI        NgoMIV      
NheI        NlaIV       NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PvuI        
PvuII       RcaI        RsrII       SacI        SacII       
SalI        SapI        Sau96I      SbfI        ScaI        
ScrFI       SexAI       SfaNI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       StyI        
SwaI        TseI        Tth111I     Van91I      XbaI        
XcmI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   25 enzymes) #
###################################

AflIII        147  ACATGT               A^C[GA][CT]GT
BalI           49  TGGCCA               TGG^CCA
BmyI          166  GGGCAC               G[AGT]GC[ACT]^C
BseRI        1941  CTCCTC               GAGGAG(10/8)
BsiYI         419  CCTTTAACTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          419  CCTTTAACTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1286I      166  GGGCAC               G[AGT]GC[ACT]^C
BspDI        1265  ATCGAT               AT^CGAT
BspLU11I      147  ACATGT               A^CATGT
BstF5I        153  GGATG                GGATG(2/0)
Cac8I         341  GCGTGC               GC[ACGT]^[ACGT]GC
ClaI         1265  ATCGAT               AT^CGAT
EaeI           49  TGGCCA               [CT]^GGCC[GA]
FokI          153  GGATG                GGATG(9/13)
HincII        190  GTTAAC               GT[CT]^[GA]AC
HindII        190  GTTAAC               GT[CT]^[GA]AC
HpaI          190  GTTAAC               GTT^AAC
MluNI          49  TGGCCA               TGG^CCA
MscI           49  TGGCCA               TGG^CCA
NspI          147  ACATGT               [GA]CATG^[CT]
PstI         1136  CTGCAG               CTGCA^G
SfcI         1136  CTGCAG               C^T[GA][CT]AG
SspI          222  AATATT               AAT^ATT
TaqI         1266  TCGA                 T^CGA
TthHB8I      1266  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   13 enzymes) #
###################################

AfaI          161  GTAC                 GT^AC
             1616  GTAC                
AlwI          932  GATCC                GGATC(4/5)
             1268  GATCC               
BbsI          287  GAAGAC               GAAGAC(2/6)
             1353  GTCTTC              
BpuAI         287  GAAGAC               GAAGAC(2/6)
             1353  GTCTTC              
DdeI           91  CTGAG                C^T[ACGT]AG
              359  CTCAG               
DpnII         932  GATC                 ^GATC
             1268  GATC                
DraI          589  TTTAAA               TTT^AAA
             1597  TTTAAA              
EarI          550  GAAGAG               CTCTTC(1/4)
              970  GAAGAG              
Ksp632I       550  GAAGAG               CTCTTC(1/4)
              970  GAAGAG              
MboI          932  GATC                 ^GATC
             1268  GATC                
NdeII         932  GATC                 ^GATC
             1268  GATC                
RsaI          161  GTAC                 GT^AC
             1616  GTAC                
Sau3AI        932  GATC                 ^GATC
             1268  GATC                


#########################################
# 3 OR MORE CUT ENZYMES (   24 enzymes) #
#########################################

AcsI        AluI        ApoI        BfaI        BpmI        
BsgI        BsrI        EcoRI       HaeIII      HindIII     
HinfI       MaeI        MaeIII      MboII       MnlI        
MseI        MslI        NlaIII      StuI        TfiI        
Tru9I       Tsp45I      Tsp509I     TspRI