Restriction map for
pF1KB7058
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# NO CUT ENZYMES ( 110 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI AlwNI
AocI Aor51HI ApaI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BanI BbeI BbrPI BbvCI
BcgI BciVI BclI BfrI BlnI
BsaAI BsaI BsaWI BseAI BsgI
BsiEI BsiWI BspDI BspEI BspHI
BspLU11I BsrBI Bst1107I BstBI BstF5I
BstZ17I Bsu36I ClaI CpoI DraI
DraIII EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoRI EcoRV
FbaI FokI FseI FspI HincII
HindII HindIII HpaI KasI KpnI
MluI MroI MslI NarI NcoI
NdeI NheI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SalI SbfI
ScaI SexAI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AcsI 180 AAATTC [GA]^AATT[CT]
Alw44I 131 GTGCAC G^TGCAC
ApaLI 131 GTGCAC G^TGCAC
ApoI 180 AAATTC [GA]^AATT[CT]
BamHI 667 GGATCC G^GATCC
BlpI 1206 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1206 GCTGAGC GC^T[ACGT]AGC
BsaBI 846 GATGTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 191 GAGACG CGTCTC(1/5)
Bsp1407I 1474 TGTACA T^GTACA
BsrDI 1834 CATTGC GCAATG(2/0)
BsrGI 1474 TGTACA T^GTACA
BssHII 14 GCGCGC G^CGCGC
BssSI 961 CACGAG CACGAG(-5/-1)
BstAPI 851 GCATCGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1731 GGTGACC G^GT[ACGT]ACC
BstPI 1731 GGTGACC G^GT[ACGT]ACC
CelII 1206 GCTGAGC GC^T[ACGT]AGC
DrdI 762 GACCCAGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 1731 GGTGACC G^GT[ACGT]ACC
EcoT22I 1710 ATGCAT ATGCA^T
KspI 153 CCGCGG CCGC^GG
MamI 846 GATGTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 632 CAATTG C^AATTG
MunI 632 CAATTG C^AATTG
NaeI 264 GCCGGC GCC^GGC
NgoMIV 264 GCCGGC G^CCGGC
NsiI 1710 ATGCAT ATGCA^T
PflMI 971 CCAAGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 290 CTGCAG CTGCA^G
PvuII 1204 CAGCTG CAG^CTG
SacII 153 CCGCGG CCGC^GG
SapI 429 GCTCTTC GCTCTTC(1/4)
SfiI 900 GGCCAACCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 58 CTCAAG C^T[CT][GA]AG
SspBI 1474 TGTACA T^GTACA
Van91I 971 CCAAGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcyI 163 GACGCC G[GA]^CG[CT]C
309 GGCGTC
BalI 544 TGGCCA TGG^CCA
899 TGGCCA
BbsI 510 GAAGAC GAAGAC(2/6)
1722 GTCTTC
BglI 901 GCCAACCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1546 GCCTACATGGC
BglII 440 AGATCT A^GATCT
1265 AGATCT
BpuAI 510 GAAGAC GAAGAC(2/6)
1722 GTCTTC
BsaHI 163 GACGCC G[GA]^CG[CT]C
309 GGCGTC
BseRI 220 GAGGAG GAGGAG(10/8)
448 GAGGAG
BsmI 1708 GAATGC GAATGC(1/-1)
1712 GCATTC
BspMI 260 ACCTGC ACCTGC(4/8)
1378 ACCTGC
BstXI 602 CCAATGGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
687 CCATGAGCCTGG
DdeI 444 CTCAG C^T[ACGT]AG
1207 CTGAG
EcoT14I 547 CCAAGG C^C[AT][AT]GG
671 CCTTGG
Hin1I 163 GACGCC G[GA]^CG[CT]C
309 GGCGTC
MaeII 194 ACGT A^CGT
1094 ACGT
MluNI 544 TGGCCA TGG^CCA
899 TGGCCA
MscI 544 TGGCCA TGG^CCA
899 TGGCCA
NspI 29 GCATGT [GA]CATG^[CT]
872 ACATGC
SacI 1166 GAGCTC GAGCT^C
1238 GAGCTC
StyI 547 CCAAGG C^C[AT][AT]GG
671 CCTTGG
TaqI 1118 TCGA T^CGA
1772 TCGA
TfiI 683 GAATC G^A[AT]TC
1449 GATTC
Tsp509I 181 AATT ^AATT
633 AATT
TthHB8I 1118 TCGA T^CGA
1772 TCGA
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# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
Asp700I AspHI AvaI AvaII BanII
BbvI BcnI BfaI BmyI BpmI
BsaJI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrFI BsrI
BssKI BstNI BstUI BstYI Cac8I
CfoI Cfr10I Cfr13I DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
FauI Fnu4HI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MseI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
PpuMI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI Tru9I TseI
Tsp45I TspRI XcmI XhoII XmaCI
XmaI XmnI