Restriction map for
pF1KB7055
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# NO CUT ENZYMES ( 133 enzymes) #
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Acc65I AccII AccIII AclI AflII
AflIII AgeI AhdI AocI Aor51HI
ApaI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
BanI BbeI BbrPI BbvCI BcgI
BcnI BfrI BglI BglII BlpI
BpmI Bpu1102I BsaBI BsaWI BseAI
BseRI BsiEI BsiWI BsoBI BspDI
BspEI BspLU11I BspMI BsrBI BsrDI
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraI DraII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO109I EcoO65I EcoRV EcoT22I
FauI FseI FspI HaeII HgaI
HhaI HinP1I KasI KpnI KspI
MamI MluI MroI MvnI NaeI
NarI NciI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SnaBI SphI Sse8387I
StuI SwaI Tth111I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AatII 1247 GACGTC GACGT^C
AccI 211 GTAGAC GT^[AC][GT]AC
AciI 101 GCGG CCGC(-3/-1)
AcyI 1247 GACGTC G[GA]^CG[CT]C
Alw44I 539 GTGCAC G^TGCAC
AlwNI 530 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 539 GTGCAC G^TGCAC
AvrII 297 CCTAGG C^CTAGG
BamHI 565 GGATCC G^GATCC
BanII 775 GAGCTC G[GA]GC[CT]^C
BlnI 297 CCTAGG C^CTAGG
BsaAI 872 CACGTA [CT]AC^GT[GA]
BsaHI 1247 GACGTC G[GA]^CG[CT]C
BsaI 341 GAGACC GGTCTC(1/5)
BsgI 719 CTGCAC GTGCAG(16/14)
BsmBI 1249 CGTCTC CGTCTC(1/5)
BsmI 261 GAATGC GAATGC(1/-1)
DraIII 557 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 897 CCATGG C^C[GA][CT]GG
EcoNI 267 CCTACATAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1172 GAATTC G^AATTC
HapII 840 CCGG C^CGG
Hin1I 1247 GACGTC G[GA]^CG[CT]C
HindIII 524 AAGCTT A^AGCTT
HpaI 607 GTTAAC GTT^AAC
HpaII 840 CCGG C^CGG
HphI 894 TCACC GGTGA(8/7)
MfeI 91 CAATTG C^AATTG
MseI 608 TTAA T^TAA
MslI 295 CACCTAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 56 CAGCTG C[AC]G^C[GT]G
MspI 840 CCGG C^CGG
MunI 91 CAATTG C^AATTG
MwoI 532 GCCTCTGGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 897 CCATGG C^CATGG
PflMI 660 CCAAAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 1589 CTGCAG CTGCA^G
PvuII 56 CAGCTG CAG^CTG
SacI 775 GAGCTC GAGCT^C
SapI 1135 GAAGAGC GCTCTTC(1/4)
SfaNI 1091 GCATC GCATC(5/9)
SfcI 1589 CTGCAG C^T[GA][CT]AG
SpeI 600 ACTAGT A^CTAGT
SspI 1663 AATATT AAT^ATT
Tru9I 608 TTAA T^TAA
Tsp45I 1656 GTCAC ^GT[GC]AC
Van91I 660 CCAAAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcsI 633 AAATTC [GA]^AATT[CT]
1172 GAATTC
ApoI 633 AAATTC [GA]^AATT[CT]
1172 GAATTC
AvaII 233 GGACC G^G[AT]CC
745 GGACC
BbsI 1723 GAAGAC GAAGAC(2/6)
867 GTCTTC
BbvI 55 GCAGC GCAGC(8/12)
935 GCAGC
BclI 222 TGATCA T^GATCA
1698 TGATCA
BpuAI 1723 GAAGAC GAAGAC(2/6)
867 GTCTTC
BsaJI 297 CCTAGG C^C[ACGT][ACGT]GG
897 CCATGG
BsmFI 66 GGGAC GGGAC(10/14)
412 GGGAC
Bsp1407I 912 TGTACA T^GTACA
1272 TGTACA
BspHI 225 TCATGA T^CATGA
1617 TCATGA
BsrGI 912 TGTACA T^GTACA
1272 TGTACA
BstYI 103 GGATCT [GA]^GATC[CT]
565 GGATCC
EcoT14I 297 CCTAGG C^C[AT][AT]GG
897 CCATGG
FbaI 222 TGATCA T^GATCA
1698 TGATCA
Fnu4HI 55 GCAGC GC^[ACGT]GC
935 GCAGC
ItaI 55 GCAGC GC^[ACGT]GC
935 GCAGC
MflI 103 GGATCT [GA]^GATC[CT]
565 GGATCC
RcaI 225 TCATGA T^CATGA
1617 TCATGA
SmlI 428 CTCAAG C^T[CT][GA]AG
1187 CTCAAG
SspBI 912 TGTACA T^GTACA
1272 TGTACA
StyI 297 CCTAGG C^C[AT][AT]GG
897 CCATGG
TaqI 1123 TCGA T^CGA
1231 TCGA
TfiI 795 GAATC G^A[AT]TC
1645 GAATC
TseI 55 GCAGC G^C[AT]GC
935 GCAGC
TspRI 1257 CAGTG CA[GC]TG[ACGT][ACGT]^
1650 CAGTG
TthHB8I 1123 TCGA T^CGA
1231 TCGA
XhoII 103 GGATCT [GA]^GATC[CT]
565 GGATCC
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# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AfaI AluI AlwI AspHI BalI
BciVI BfaI BmyI BsiHKAI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII EaeI EarI EcoRII FokI
HaeIII HincII HindII HinfI Ksp632I
MaeI MaeII MaeIII MboI MboII
MluNI MnlI MscI MvaI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SexAI Tsp509I XcmI