Restriction map for
pF1KB7050
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# NO CUT ENZYMES ( 113 enzymes) #
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AatII AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI AlwNI
ApaI ApoI AscI AseI AsnI
AspEI BalI BamHI BbeI BbrPI
BbsI BbvCI BclI BfrI BglII
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaWI BseAI BsgI BsiWI BsmBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrGI BssHII BssSI Bst1107I
BstAPI BstEII BstPI BstXI BstZ17I
CelII ClaI CpoI DrdI Eam1105I
EcoO65I EcoRI EcoT22I FbaI FseI
HincII HindII HindIII HpaI KasI
MaeII MamI MfeI MluI MluNI
MroI MscI MslI MunI NarI
NdeI NheI NruI NsiI NspI
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SalI
SapI SbfI ScaI SexAI SfiI
SfoI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 46 enzymes) #
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Acc65I 108 GGTACC G^GTACC
AcyI 279 GACGCC G[GA]^CG[CT]C
Alw44I 375 GTGCAC G^TGCAC
AocI 652 CCTGAGG CC^T[ACGT]AGG
ApaLI 375 GTGCAC G^TGCAC
Asp700I 1036 GAAGGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 108 GGTACC G^GTACC
AspI 960 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 425 TGCGCA TGC^GCA
AvrII 741 CCTAGG C^CTAGG
BanII 487 GGGCTC G[GA]GC[CT]^C
BcgI 377 GCACCCTGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 827 GTATCC GTATCC(6/5)
BglI 412 GCCCAGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 741 CCTAGG C^CTAGG
BsaHI 279 GACGCC G[GA]^CG[CT]C
BsaI 26 GAGACC GGTCTC(1/5)
BsmI 1040 GCATTC GAATGC(1/-1)
BspMI 123 GCAGGT ACCTGC(4/8)
BstBI 628 TTCGAA TT^CGAA
BstYI 981 AGATCC [GA]^GATC[CT]
Bsu36I 652 CCTGAGG CC^T[ACGT]AGG
DraI 1089 TTTAAA TTT^AAA
DraII 877 AGGTCCT [GA]G^G[ACGT]CC[CT]
DraIII 11 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 652 CCTGAGG CC^T[ACGT]AGG
EcoNI 1288 CCTAGAGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 877 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRV 991 GATATC GAT^ATC
FspI 425 TGCGCA TGC^GCA
Hin1I 279 GACGCC G[GA]^CG[CT]C
KpnI 108 GGTACC GGTAC^C
MflI 981 AGATCC [GA]^GATC[CT]
NcoI 306 CCATGG C^CATGG
NspV 628 TTCGAA TT^CGAA
PflFI 960 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 306 CCATGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 877 AGGTCCT [GA]G^G[AT]CC[CT]
PstI 1328 CTGCAG CTGCA^G
SfuI 628 TTCGAA TT^CGAA
TaqI 629 TCGA T^CGA
Tth111I 960 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 629 TCGA T^CGA
Van91I 306 CCATGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 981 AGATCC [GA]^GATC[CT]
XmnI 1036 GAAGGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AlwI 982 GATCC GGATC(4/5)
745 GGATC
Aor51HI 321 AGCGCT AGC^GCT
338 AGCGCT
AspHI 375 GTGCAC G[AT]GC[AT]^C
480 GTGCTC
AvaII 878 GGTCC G^G[AT]CC
1068 GGTCC
BanI 108 GGTACC G^G[CT][GA]CC
589 GGTGCC
BpmI 238 CTGGAG CTGGAG(16/14)
845 CTGGAG
BsiHKAI 375 GTGCAC G[AT]GC[AT]^C
480 GTGCTC
BsrBI 4 GAGCGG CCGCTC(-3/-3)
243 GAGCGG
Eco47III 321 AGCGCT AGC^GCT
338 AGCGCT
HgaI 279 GACGC GACGC(5/10)
401 GCGTC
KspI 176 CCGCGG CCGC^GG
274 CCGCGG
MseI 1090 TTAA T^TAA
1309 TTAA
NaeI 225 GCCGGC GCC^GGC
1028 GCCGGC
NgoMIV 225 GCCGGC G^CCGGC
1028 GCCGGC
SacII 176 CCGCGG CCGC^GG
274 CCGCGG
SfaNI 120 GATGC GCATC(5/9)
514 GCATC
SfcI 466 CTACAG C^T[GA][CT]AG
1328 CTGCAG
SmaI 232 CCCGGG CCC^GGG
458 CCCGGG
Tru9I 1090 TTAA T^TAA
1309 TTAA
Tsp45I 9 GTCAC ^GT[GC]AC
1356 GTCAC
Tsp509I 1015 AATT ^AATT
1191 AATT
XmaCI 232 CCCGGG C^CCGGG
458 CCCGGG
XmaI 232 CCCGGG C^CCGGG
458 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AccII AciI AfaI AluI AvaI
BbvI BcnI BfaI BmyI BsaJI
BseRI BsiEI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrFI BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DsaI EaeI EagI EarI EclXI
Eco52I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NotI PleI RsaI Sau3AI Sau96I
ScrFI SmlI StyI TfiI TseI
TspRI