Restriction map for
pF1KB7047
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# NO CUT ENZYMES ( 140 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI ApaI ApaLI AscI
Asp718I AspEI AvaI AvaII AviII
AvrII BalI BamHI BanI BbeI
BbrPI BbvCI BcgI BciVI BclI
BcnI BfrI BglI BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsiEI BsiWI BsoBI
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrFI BssHII BssSI Bst1107I
BstAPI BstUI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraII DraIII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco52I Eco81I EcoNI EcoO109I
EcoRV FauI FbaI FseI FspI
HapII HgaI Hin1I HincII HindII
HindIII HpaI HpaII KasI KpnI
KspI MamI MluI MluNI MroI
MscI MspI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NlaIV NotI NruI PacI PaeR7I
PinAI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccI 307 GTCTAC GT^[AC][GT]AC
AlwI 96 GGATC GGATC(4/5)
AlwNI 1376 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 1085 AGCGCT AGC^GCT
AseI 1266 ATTAAT AT^TAAT
AsnI 1266 ATTAAT AT^TAAT
AspHI 1441 GAGCTC G[AT]GC[AT]^C
AspI 82 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanII 1441 GAGCTC G[GA]GC[CT]^C
BglII 1287 AGATCT A^GATCT
BmyI 1441 GAGCTC G[AGT]GC[ACT]^C
BsgI 737 GTGCAG GTGCAG(16/14)
BsiHKAI 1441 GAGCTC G[AT]GC[AT]^C
BsmBI 1018 GAGACG CGTCTC(1/5)
Bsp1286I 1441 GAGCTC G[AGT]GC[ACT]^C
Bsp1407I 158 TGTACA T^GTACA
BspMI 367 ACCTGC ACCTGC(4/8)
BsrGI 158 TGTACA T^GTACA
BstBI 951 TTCGAA TT^CGAA
BstXI 1243 CCAAATTATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI 132 TTTAAA TTT^AAA
Eco47III 1085 AGCGCT AGC^GCT
EcoT14I 698 CCTTGG C^C[AT][AT]GG
EcoT22I 989 ATGCAT ATGCA^T
HaeII 1085 AGCGCT [GA]GCGC^[CT]
MaeII 623 ACGT A^CGT
MfeI 530 CAATTG C^AATTG
MspA1I 43 CAGCTG C[AC]G^C[GT]G
MunI 530 CAATTG C^AATTG
NsiI 989 ATGCAT ATGCA^T
NspI 252 ACATGC [GA]CATG^[CT]
NspV 951 TTCGAA TT^CGAA
PflFI 82 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 814 CCAAACAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 1266 ATTAAT AT^TAAT
PstI 649 CTGCAG CTGCA^G
PvuII 43 CAGCTG CAG^CTG
SacI 1441 GAGCTC GAGCT^C
SfcI 649 CTGCAG C^T[GA][CT]AG
SfuI 951 TTCGAA TT^CGAA
SspBI 158 TGTACA T^GTACA
StyI 698 CCTTGG C^C[AT][AT]GG
TaqI 952 TCGA T^CGA
Tth111I 82 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 952 TCGA T^CGA
Van91I 814 CCAAACAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 99 TCTAGA T^CTAGA
XcmI 14 CCAAGAATTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AciI 732 CCGC CCGC(-3/-1)
1329 CCGC
AfaI 159 GTAC GT^AC
540 GTAC
BbsI 79 GAAGAC GAAGAC(2/6)
1123 GAAGAC
BpuAI 79 GAAGAC GAAGAC(2/6)
1123 GAAGAC
BseRI 516 CTCCTC GAGGAG(10/8)
1444 CTCCTC
BsmI 214 GAATGC GAATGC(1/-1)
987 GAATGC
BstEII 9 GGTCACC G^GT[ACGT]ACC
763 GGTGACC
BstF5I 1341 GGATG GGATG(2/0)
1392 GGATG
BstPI 9 GGTCACC G^GT[ACGT]ACC
763 GGTGACC
BstYI 96 GGATCT [GA]^GATC[CT]
1287 AGATCT
CfoI 1086 GCGC GCG^C
1418 GCGC
EarI 232 GAAGAG CTCTTC(1/4)
1186 GAAGAG
EcoO65I 9 GGTCACC G^GT[ACGT]ACC
763 GGTGACC
EcoRI 18 GAATTC G^AATTC
511 GAATTC
FokI 1341 GGATG GGATG(9/13)
1392 GGATG
HhaI 1086 GCGC GCG^C
1418 GCGC
HinP1I 1086 GCGC G^CGC
1418 GCGC
Ksp632I 232 GAAGAG CTCTTC(1/4)
1186 GAAGAG
MflI 96 GGATCT [GA]^GATC[CT]
1287 AGATCT
MslI 184 CACCTAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1128 CACTGCTGTG
MwoI 256 GCTGACCAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1418 GCGCAGTCAGC
NlaIII 253 CATG CATG^
1141 CATG
PleI 326 GACTC GAGTC(4/5)
606 GAGTC
RsaI 159 GTAC GT^AC
540 GTAC
XhoII 96 GGATCT [GA]^GATC[CT]
1287 AGATCT
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# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AcsI AluI ApoI Asp700I BbvI
BfaI BpmI BsaJI BsiYI BslI
BsmAI BsmFI BsrI BssKI BstNI
Cac8I Cfr13I DdeI DpnII EcoRII
Fnu4HI HaeIII HinfI HphI ItaI
MaeI MaeIII MboI MboII MnlI
MseI MvaI NdeII Sau3AI Sau96I
ScrFI SfaNI SmlI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XmnI