Restriction map for
pF1KB7043
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# NO CUT ENZYMES ( 109 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AflIII AgeI Alw44I Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspI AvrII BalI
BamHI BcgI BciVI BclI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaBI BsgI BsmI BspDI BspHI
BspLU11I BspMI Bst1107I BstBI BstEII
BstPI BstXI BstZ17I CelII ClaI
CpoI DraI DraIII DrdI EarI
Eco47III EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI HincII HindII
HindIII HpaI KpnI Ksp632I MaeIII
MamI MfeI MluI MluNI MscI
MunI NcoI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmeI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SalI SapI SbfI SexAI
SfiI SfuI SnaBI SpeI Sse8387I
SspI StuI SwaI Tsp45I Tsp509I
Tth111I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccIII 678 TCCGGA T^CCGGA
AhdI 297 GACGCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 79 CCTGAGG CC^T[ACGT]AGG
AspEI 297 GACGCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 860 TGCGCA TGC^GCA
BbrPI 469 CACGTG CAC^GTG
BbvCI 773 CCTCAGC CCTCAGC(-5/-2)
BfaI 452 CTAG C^TAG
BsaAI 469 CACGTG [CT]AC^GT[GA]
BsaI 979 GAGACC GGTCTC(1/5)
BsaWI 678 TCCGGA [AT]^CCGG[AT]
BseAI 678 TCCGGA T^CCGGA
BsiWI 269 CGTACG C^GTACG
BsmBI 91 GAGACG CGTCTC(1/5)
Bsp1407I 1399 TGTACA T^GTACA
BspEI 678 TCCGGA T^CCGGA
BsrDI 1329 CATTGC GCAATG(2/0)
BsrGI 1399 TGTACA T^GTACA
BssHII 69 GCGCGC G^CGCGC
BssSI 651 CACGAG CACGAG(-5/-1)
BstAPI 846 GCAGCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 956 AGATCC [GA]^GATC[CT]
Bsu36I 79 CCTGAGG CC^T[ACGT]AGG
Eam1105I 297 GACGCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 79 CCTGAGG CC^T[ACGT]AGG
FspI 860 TGCGCA TGC^GCA
KspI 418 CCGCGG CCGC^GG
MaeI 452 CTAG C^TAG
MflI 956 AGATCC [GA]^GATC[CT]
MroI 678 TCCGGA T^CCGGA
NaeI 1035 GCCGGC GCC^GGC
NgoMIV 1035 GCCGGC G^CCGGC
PmaCI 469 CACGTG CAC^GTG
PmlI 469 CACGTG CAC^GTG
PvuII 847 CAGCTG CAG^CTG
SacII 418 CCGCGG CCGC^GG
ScaI 809 AGTACT AGT^ACT
SgrAI 1034 CGCCGGCG C[GA]^CCGG[CT]G
SspBI 1399 TGTACA T^GTACA
XcmI 499 CCAGGGGGCGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 956 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AlwNI 826 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
844 CAGCAGCTG
ApaI 695 GGGCCC GGGCC^C
1261 GGGCCC
AspHI 654 GAGCAC G[AT]GC[AT]^C
1153 GTGCTC
BbeI 276 GGCGCC GGCGC^C
285 GGCGCC
BseRI 714 CTCCTC GAGGAG(10/8)
682 GAGGAG
BsiEI 688 CGGCCG CG[GA][CT]^CG
730 CGGCCG
BsiHKAI 654 GAGCAC G[AT]GC[AT]^C
1153 GTGCTC
BsrFI 732 GCCGGT [GA]^CCGG[CT]
1035 GCCGGC
BsrI 18 CCAGT ACTGG(1/-1)
1209 CCAGT
Cfr10I 732 GCCGGT [GA]^CCGG[CT]
1035 GCCGGC
EagI 688 CGGCCG C^GGCCG
730 CGGCCG
EclXI 688 CGGCCG C^GGCCG
730 CGGCCG
Eco52I 688 CGGCCG C^GGCCG
730 CGGCCG
EcoT14I 1124 CCAAGG C^C[AT][AT]GG
1257 CCAAGG
KasI 276 GGCGCC G^GCGCC
285 GGCGCC
MseI 1160 TTAA T^TAA
1286 TTAA
MslI 45 CATGCTTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1242 CACCAGGATG
NarI 276 GGCGCC GG^CGCC
285 GGCGCC
PflMI 843 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1097 CCAAATGCTGG
SfoI 276 GGCGCC GGC^GCC
285 GGCGCC
SmaI 139 CCCGGG CCC^GGG
337 CCCGGG
SphI 245 GCATGC GCATG^C
944 GCATGC
StyI 1124 CCAAGG C^C[AT][AT]GG
1257 CCAAGG
TaqI 917 TCGA T^CGA
1280 TCGA
TfiI 1274 GATTC G^A[AT]TC
1310 GATTC
Tru9I 1160 TTAA T^TAA
1286 TTAA
TthHB8I 917 TCGA T^CGA
1280 TCGA
Van91I 843 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1097 CCAAATGCTGG
XmaCI 139 CCCGGG C^CCGGG
337 CCCGGG
XmaI 139 CCCGGG C^CCGGG
337 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AvaI AvaII BanI BanII
BbsI BbvI BcnI BmyI BpmI
BpuAI BsaHI BsaJI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrBI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EcoO109I EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI MaeII MboI
MboII MnlI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI TseI TspRI