##################################
# NO CUT ENZYMES ( 117 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AgeI AocI
Aor51HI ApaI ApoI AscI AseI
AsnI Asp700I Asp718I AspI AvrII
BamHI BanI BbeI BbvCI BcgI
BciVI BclI BfaI BfrI BglI
BglII BlnI BsaBI BsaHI BseAI
BsiWI BsmI BspDI BspEI BspLU11I
BsrBI BsrDI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I Bsu36I ClaI DraI DrdI
EagI EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV FbaI FseI
Hin1I HindIII HpaI KasI KpnI
KspI MaeI MamI MfeI MluI
MroI MseI MunI NarI NcoI
NdeI NheI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmeI
PshAI PshBI Psp1406I PstI PvuII
SacII SalI SbfI SexAI SfcI
SfiI SfoI SfuI SgrAI SnaBI
SpeI Sse8387I SspI SwaI TfiI
Tru9I Tsp509I Tth111I XbaI XcmI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AflIII 1202 ACGTGT A^C[GA][CT]GT
AhdI 1113 GACCTCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 222 GTGCAC G^TGCAC
AlwI 876 GATCC GGATC(4/5)
ApaLI 222 GTGCAC G^TGCAC
AspEI 1113 GACCTCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 760 CCCGGG C^[CT]CG[GA]G
AviII 644 TGCGCA TGC^GCA
BanII 241 GAGCTC G[GA]GC[CT]^C
BbrPI 1201 CACGTG CAC^GTG
BbsI 454 GAAGAC GAAGAC(2/6)
BpuAI 454 GAAGAC GAAGAC(2/6)
BsaWI 192 ACCGGA [AT]^CCGG[AT]
BsgI 1036 CTGCAC GTGCAG(16/14)
BsiEI 540 CGATCG CG[GA][CT]^CG
BsmBI 1354 GAGACG CGTCTC(1/5)
BsmFI 105 GTCCC GGGAC(10/14)
BsoBI 760 CCCGGG C^[CT]CG[GA]G
BspHI 1127 TCATGA T^CATGA
BsrFI 916 GCCGGC [GA]^CCGG[CT]
BstYI 875 AGATCC [GA]^GATC[CT]
Cfr10I 916 GCCGGC [GA]^CCGG[CT]
CpoI 1186 CGGTCCG CG^G[AT]CCG
Eam1105I 1113 GACCTCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I 799 ATGCAT ATGCA^T
FspI 644 TGCGCA TGC^GCA
HaeII 350 AGCGCC [GA]GCGC^[CT]
HincII 532 GTCAAC GT[CT]^[GA]AC
HindII 532 GTCAAC GT[CT]^[GA]AC
HinfI 828 GACTC G^A[ACGT]TC
MflI 875 AGATCC [GA]^GATC[CT]
NaeI 916 GCCGGC GCC^GGC
NgoMIV 916 GCCGGC G^CCGGC
NsiI 799 ATGCAT ATGCA^T
PleI 828 GACTC GAGTC(4/5)
PmaCI 1201 CACGTG CAC^GTG
PmlI 1201 CACGTG CAC^GTG
PvuI 540 CGATCG CGAT^CG
RcaI 1127 TCATGA T^CATGA
RsrII 1186 CGGTCCG CG^G[AT]CCG
SacI 241 GAGCTC GAGCT^C
ScaI 1304 AGTACT AGT^ACT
SmaI 760 CCCGGG CCC^GGG
SmlI 397 CTCAAG C^T[CT][GA]AG
XhoII 875 AGATCC [GA]^GATC[CT]
XmaCI 760 CCCGGG C^CCGGG
XmaI 760 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AlwNI 334 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
937 CAGGTCCTG
BalI 278 TGGCCA TGG^CCA
692 TGGCCA
BlpI 87 GCTGAGC GC^T[ACGT]AGC
243 GCTCAGC
Bpu1102I 87 GCTGAGC GC^T[ACGT]AGC
243 GCTCAGC
BsaAI 898 TACGTG [CT]AC^GT[GA]
1201 CACGTG
Bsp1407I 485 TGTACA T^GTACA
1002 TGTACA
BsrGI 485 TGTACA T^GTACA
1002 TGTACA
BsrI 528 ACTGG ACTGG(1/-1)
1042 CCAGT
CelII 87 GCTGAGC GC^T[ACGT]AGC
243 GCTCAGC
DsaI 236 CCACGG C^C[GA][CT]GG
908 CCGTGG
EaeI 278 TGGCCA [CT]^GGCC[GA]
692 TGGCCA
EcoNI 327 CCTGAACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1140 CCTCATGCAGG
EcoT14I 287 CCAAGG C^C[AT][AT]GG
626 CCAAGG
MluNI 278 TGGCCA TGG^CCA
692 TGGCCA
MscI 278 TGGCCA TGG^CCA
692 TGGCCA
SapI 765 GAAGAGC GCTCTTC(1/4)
381 GCTCTTC
SspBI 485 TGTACA T^GTACA
1002 TGTACA
StyI 287 CCAAGG C^C[AT][AT]GG
626 CCAAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AccII AciI AfaI AluI AspHI
AvaII BbvI BcnI BmyI BpmI
BsaI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI Bsp1286I BspMI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII DraII DraIII
EarI EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MnlI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PpuMI RsaI Sau3AI
Sau96I ScrFI SfaNI SphI StuI
TaqI TseI Tsp45I TspRI TthHB8I
Van91I