Restriction map for
pF1KB7038
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# NO CUT ENZYMES ( 115 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
AlwNI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BamHI BbrPI BbsI BbvCI
BciVI BclI BfaI BfrI BlnI
BpuAI BsaBI BsaI BsaWI BseAI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BsrDI BsrGI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I ClaI CpoI DraI DraIII
DrdI Eam1105I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FspI HincII
HindII HindIII HpaI KpnI MaeI
MamI MfeI MluI MroI MseI
MunI NdeI NheI NruI NsiI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacI SalI SapI SbfI ScaI
SexAI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TfiI Tru9I Tsp509I Tth111I XbaI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AlwI 563 GGATC GGATC(4/5)
AocI 1078 CCTGAGG CC^T[ACGT]AGG
Asp700I 756 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 937 GAGCAC G[AT]GC[AT]^C
BcgI 719 GCAACATGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 551 AGATCT A^GATCT
BpmI 42 CTGGAG CTGGAG(16/14)
BsgI 703 CTGCAC GTGCAG(16/14)
BsiHKAI 937 GAGCAC G[AT]GC[AT]^C
BsiWI 200 CGTACG C^GTACG
BsmAI 886 GAGAC GTCTC(1/5)
BssSI 28 CACGAG CACGAG(-5/-1)
BstYI 551 AGATCT [GA]^GATC[CT]
Bsu36I 1078 CCTGAGG CC^T[ACGT]AGG
DraII 950 GGGGCCT [GA]G^G[ACGT]CC[CT]
EarI 574 CTCTTC CTCTTC(1/4)
Eco81I 1078 CCTGAGG CC^T[ACGT]AGG
EcoO109I 950 GGGGCCT [GA]G^G[ACGT]CC[CT]
FseI 875 GGCCGGCC GGCCGG^CC
Ksp632I 574 CTCTTC CTCTTC(1/4)
KspI 1034 CCGCGG CCGC^GG
MaeIII 1279 GTCAC ^GT[ACGT]AC
MflI 551 AGATCT [GA]^GATC[CT]
PaeR7I 915 CTCGAG C^TCGAG
PvuII 778 CAGCTG CAG^CTG
SacII 1034 CCGCGG CCGC^GG
SfcI 450 CTACAG C^T[GA][CT]AG
SfiI 279 GGCCGGGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Tsp45I 1279 GTCAC ^GT[GC]AC
TspRI 559 CAGTG CA[GC]TG[ACGT][ACGT]^
XhoI 915 CTCGAG C^TCGAG
XhoII 551 AGATCT [GA]^GATC[CT]
XmnI 756 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
Aor51HI 602 AGCGCT AGC^GCT
758 AGCGCT
ApaI 1050 GGGCCC GGGCC^C
1272 GGGCCC
BalI 509 TGGCCA TGG^CCA
1265 TGGCCA
BlpI 543 GCTGAGC GC^T[ACGT]AGC
987 GCTGAGC
Bpu1102I 543 GCTGAGC GC^T[ACGT]AGC
987 GCTGAGC
BsaAI 123 CACGTA [CT]AC^GT[GA]
202 TACGTG
BseRI 75 CTCCTC GAGGAG(10/8)
1365 CTCCTC
BspLU11I 191 ACATGT A^CATGT
722 ACATGT
BspMI 743 ACCTGC ACCTGC(4/8)
1043 ACCTGC
BsrI 41 ACTGG ACTGG(1/-1)
558 CCAGT
CelII 543 GCTGAGC GC^T[ACGT]AGC
987 GCTGAGC
DpnII 552 GATC ^GATC
564 GATC
Eco47III 602 AGCGCT AGC^GCT
758 AGCGCT
MaeII 124 ACGT A^CGT
203 ACGT
MboI 552 GATC ^GATC
564 GATC
MboII 15 GAAGA GAAGA(8/7)
575 TCTTC
MluNI 509 TGGCCA TGG^CCA
1265 TGGCCA
MscI 509 TGGCCA TGG^CCA
1265 TGGCCA
NdeII 552 GATC ^GATC
564 GATC
NotI 142 GCGGCCGC GC^GGCCGC
826 GCGGCCGC
PflMI 1041 CCACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1191 CCACAAAATGG
Sau3AI 552 GATC ^GATC
564 GATC
SfaNI 534 GATGC GCATC(5/9)
1224 GATGC
SmaI 1015 CCCGGG CCC^GGG
1065 CCCGGG
StuI 842 AGGCCT AGG^CCT
1208 AGGCCT
TaqI 728 TCGA T^CGA
916 TCGA
TthHB8I 728 TCGA T^CGA
916 TCGA
Van91I 1041 CCACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1191 CCACAAAATGG
XcmI 257 CCATGGCGGGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
359 CCATGGGCGGCCTGG
XmaCI 1015 CCCGGG C^CCGGG
1065 CCCGGG
XmaI 1015 CCCGGG C^CCGGG
1065 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AvaI AvaII BanI BanII
BbeI BbvI BcnI BglI BmyI
BsaHI BsaJI BsiEI BsiYI BslI
BsmFI BsoBI Bsp1286I BsrBI BsrFI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr10I Cfr13I DdeI DsaI
EaeI EagI EclXI Eco52I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI MnlI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NcoI NgoMIV NlaIII NlaIV
NspI PleI RsaI Sau96I ScrFI
SfoI SmlI StyI TseI