Restriction map for
pF1KB7035
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# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII AccIII AclI AcsI AflII
AflIII AgeI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI AspI AviII AvrII
BalI BbrPI BbvCI BcgI BciVI
BclI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaWI BseAI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I CelII ClaI
CpoI DraIII DrdI DsaI EagI
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HpaI KspI MaeI MamI
MfeI MluI MluNI MroI MscI
MunI NcoI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacII SalI SbfI
ScaI SexAI SfiI SfuI SmaI
SmlI SnaBI SpeI Sse8387I SspBI
SspI StuI SwaI TfiI Tsp509I
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
###################################
Acc65I 231 GGTACC G^GTACC
AccI 631 GTAGAC GT^[AC][GT]AC
Asp700I 969 GAAACAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 231 GGTACC G^GTACC
AvaI 1062 CCCGAG C^[CT]CG[GA]G
BamHI 73 GGATCC G^GATCC
BanII 705 GAGCTC G[GA]GC[CT]^C
BbeI 751 GGCGCC GGCGC^C
BpmI 669 CTCCAG CTGGAG(16/14)
BsmFI 481 GTCCC GGGAC(10/14)
BsoBI 1062 CCCGAG C^[CT]CG[GA]G
BsrDI 1017 CATTGC GCAATG(2/0)
BsrI 1010 ACTGG ACTGG(1/-1)
BstXI 741 CCAGGAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 73 GGATCC [GA]^GATC[CT]
DraI 186 TTTAAA TTT^AAA
DraII 1087 GGGTCCT [GA]G^G[ACGT]CC[CT]
EaeI 119 TGGCCG [CT]^GGCC[GA]
EcoNI 126 CCTCCCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1087 GGGTCCT [GA]G^G[ACGT]CC[CT]
HincII 952 GTCAAC GT[CT]^[GA]AC
HindII 952 GTCAAC GT[CT]^[GA]AC
HindIII 265 AAGCTT A^AGCTT
HinfI 382 GAGTC G^A[ACGT]TC
KasI 751 GGCGCC G^GCGCC
KpnI 231 GGTACC GGTAC^C
MflI 73 GGATCC [GA]^GATC[CT]
MspA1I 682 CAGCGG C[AC]G^C[GT]G
NaeI 917 GCCGGC GCC^GGC
NarI 751 GGCGCC GG^CGCC
NgoMIV 917 GCCGGC G^CCGGC
PleI 382 GAGTC GAGTC(4/5)
PpuMI 1087 GGGTCCT [GA]G^G[AT]CC[CT]
PstI 883 CTGCAG CTGCA^G
SacI 705 GAGCTC GAGCT^C
SfaNI 677 GCATC GCATC(5/9)
SfcI 883 CTGCAG C^T[GA][CT]AG
SfoI 751 GGCGCC GGC^GCC
SgrAI 916 CGCCGGCG C[GA]^CCGG[CT]G
SphI 1130 GCATGC GCATG^C
TaqI 413 TCGA T^CGA
TthHB8I 413 TCGA T^CGA
XhoII 73 GGATCC [GA]^GATC[CT]
XmnI 969 GAAACAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccII 90 CGCG CG^CG
255 CGCG
AcyI 751 GGCGCC G[GA]^CG[CT]C
920 GGCGTC
AhdI 100 GACTGTCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
212 GACCCGATGTC
AspEI 100 GACTGTCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
212 GACCCGATGTC
BanI 231 GGTACC G^G[CT][GA]CC
751 GGCGCC
BcnI 890 CCCGG CC^[GC]GG
1085 CCGGG
BsaHI 751 GGCGCC G[GA]^CG[CT]C
920 GGCGTC
BsaI 210 GAGACC GGTCTC(1/5)
1188 GAGACC
BsrFI 228 GCCGGT [GA]^CCGG[CT]
917 GCCGGC
BstUI 90 CGCG CG^CG
255 CGCG
Cfr10I 228 GCCGGT [GA]^CCGG[CT]
917 GCCGGC
Eam1105I 100 GACTGTCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
212 GACCCGATGTC
HaeII 243 GGCGCT [GA]GCGC^[CT]
751 GGCGCC
Hin1I 751 GGCGCC G[GA]^CG[CT]C
920 GGCGTC
MseI 187 TTAA T^TAA
589 TTAA
MslI 1194 CATGTCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1251 CATGATCGTG
MvnI 90 CGCG CG^CG
255 CGCG
NciI 890 CCCGG CC^[GC]GG
1085 CCGGG
PshAI 504 GACCAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1190 GACCCATGTC
SapI 642 GAAGAGC GCTCTTC(1/4)
468 GCTCTTC
Tru9I 187 TTAA T^TAA
589 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaII BbsI BbvI BmyI BpuAI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI Bsp1286I BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII EarI EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MnlI MspI MvaI MwoI NdeII
NlaIII NlaIV NspI RsaI Sau3AI
Sau96I ScrFI StyI TseI Tsp45I
TspRI