Restriction map for
pF1KB5688
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# NO CUT ENZYMES ( 97 enzymes) #
##################################
Acc65I AccIII AclI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AviII AvrII BamHI
BclI BfrI BglI BlnI BsaBI
BsaWI BseAI BsiWI BsmBI Bsp1407I
BspDI BspEI BspLU11I BspMI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I ClaI CpoI DraI
DraII DraIII DrdI Eam1105I Eco47III
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV FbaI FseI FspI HpaI
KpnI MamI MfeI MluI MroI
MunI NdeI NheI NruI NspV
PacI PflFI PinAI PmeI PpuMI
PshBI Psp1406I PvuI RsrII SalI
SbfI SexAI SfiI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
SspI SwaI Tth111I XbaI XcmI
XmaCI XmaI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AatII 1585 GACGTC GACGT^C
AccI 574 GTAGAC GT^[AC][GT]AC
AccII 18 CGCG CG^CG
ApaI 1839 GGGCCC GGGCC^C
BalI 1904 TGGCCA TGG^CCA
BbeI 72 GGCGCC GGCGC^C
BbrPI 1432 CACGTG CAC^GTG
BbvCI 1941 GCTGAGG CCTCAGC(-5/-2)
BciVI 282 GTATCC GTATCC(6/5)
BfaI 430 CTAG C^TAG
BglII 732 AGATCT A^GATCT
BlpI 142 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 142 GCTGAGC GC^T[ACGT]AGC
BsaAI 1432 CACGTG [CT]AC^GT[GA]
BsgI 1538 GTGCAG GTGCAG(16/14)
BsiEI 20 CGGCCG CG[GA][CT]^CG
BsmI 1295 GCATTC GAATGC(1/-1)
BspHI 909 TCATGA T^CATGA
BsrFI 91 GCCGGC [GA]^CCGG[CT]
BssSI 400 CTCGTG CACGAG(-5/-1)
BstAPI 112 GCAGCCAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 18 CGCG CG^CG
BstXI 579 CCAGTTCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 142 GCTGAGC GC^T[ACGT]AGC
Cfr10I 91 GCCGGC [GA]^CCGG[CT]
EagI 20 CGGCCG C^GGCCG
EclXI 20 CGGCCG C^GGCCG
Eco52I 20 CGGCCG C^GGCCG
EcoT22I 181 ATGCAT ATGCA^T
HgaI 2013 GCGTC GACGC(5/10)
HincII 1381 GTTGAC GT[CT]^[GA]AC
HindII 1381 GTTGAC GT[CT]^[GA]AC
HindIII 1148 AAGCTT A^AGCTT
KasI 72 GGCGCC G^GCGCC
KspI 17 CCGCGG CCGC^GG
MaeI 430 CTAG C^TAG
MluNI 1904 TGGCCA TGG^CCA
MscI 1904 TGGCCA TGG^CCA
MvnI 18 CGCG CG^CG
NaeI 91 GCCGGC GCC^GGC
NarI 72 GGCGCC GG^CGCC
NgoMIV 91 GCCGGC G^CCGGC
NotI 19 GCGGCCGC GC^GGCCGC
NsiI 181 ATGCAT ATGCA^T
PaeR7I 1816 CTCGAG C^TCGAG
PflMI 1134 CCAAGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1432 CACGTG CAC^GTG
PmlI 1432 CACGTG CAC^GTG
PstI 974 CTGCAG CTGCA^G
RcaI 909 TCATGA T^CATGA
SacII 17 CCGCGG CCGC^GG
ScaI 266 AGTACT AGT^ACT
SfoI 72 GGCGCC GGC^GCC
SmlI 1816 CTCGAG C^T[CT][GA]AG
Tsp45I 2015 GTCAC ^GT[GC]AC
Van91I 1134 CCAAGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1816 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcyI 72 GGCGCC G[GA]^CG[CT]C
1585 GACGTC
AfaI 267 GTAC GT^AC
1480 GTAC
AlwNI 1114 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG
1180 CAGCAGCTG
Asp700I 253 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
256 GAAGTTCTTC
AvaI 1205 CCCGAG C^[CT]CG[GA]G
1816 CTCGAG
BbsI 691 GAAGAC GAAGAC(2/6)
739 GAAGAC
BcgI 102 CGAGGCTCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
966 CGAGACCTCTGC
BpuAI 691 GAAGAC GAAGAC(2/6)
739 GAAGAC
BsaHI 72 GGCGCC G[GA]^CG[CT]C
1585 GACGTC
BsoBI 1205 CCCGAG C^[CT]CG[GA]G
1816 CTCGAG
BsrBI 463 GAGCGG CCGCTC(-3/-3)
1825 GAGCGG
CfoI 73 GCGC GCG^C
85 GCGC
HaeII 72 GGCGCC [GA]GCGC^[CT]
84 GGCGCT
HhaI 73 GCGC GCG^C
85 GCGC
Hin1I 72 GGCGCC G[GA]^CG[CT]C
1585 GACGTC
HinP1I 73 GCGC G^CGC
85 GCGC
MaeII 1433 ACGT A^CGT
1586 ACGT
MaeIII 382 GTTAC ^GT[ACGT]AC
2015 GTCAC
PshAI 1228 GACCGCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1855 GACTTCAGTC
RsaI 267 GTAC GT^AC
1480 GTAC
SacI 1519 GAGCTC GAGCT^C
1596 GAGCTC
SapI 1822 GAAGAGC GCTCTTC(1/4)
2008 GAAGAGC
SfcI 292 CTACAG C^T[GA][CT]AG
974 CTGCAG
SphI 554 GCATGC GCATG^C
990 GCATGC
StuI 1304 AGGCCT AGG^CCT
1812 AGGCCT
XmnI 253 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
256 GAAGTTCTTC
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# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
AspHI AvaII BanI BanII BbvI
BcnI BmyI BpmI BsaI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrDI BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DsaI EaeI EarI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI Ksp632I MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PleI PvuII
Sau3AI Sau96I ScrFI SfaNI StyI
TaqI TfiI Tru9I TseI Tsp509I
TspRI TthHB8I XhoII