Restriction map for
pF1KB5685
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# NO CUT ENZYMES ( 131 enzymes) #
##################################
AatII Acc65I AclI AcsI AcyI
AflII AgeI AhdI Aor51HI ApaI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BamHI BbeI BbrPI BcgI BciVI
BcnI BfrI BglII BlnI BlpI
Bpu1102I BsaBI BsaHI BsaI BsgI
BsiEI BsiWI BsmBI BspDI BspHI
BsrBI BsrFI BssHII Bst1107I BstBI
BstEII BstPI BstXI BstZ17I CelII
Cfr10I ClaI CpoI DraI DraII
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
Hin1I HindIII HpaI KasI KpnI
KspI MaeIII MamI MfeI MluI
MseI MslI MunI NaeI NarI
NciI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshBI Psp1406I PvuI RcaI
RsrII SacII SapI SbfI ScaI
SexAI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspI SwaI Tru9I Tsp45I Tsp509I
Tth111I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccI 919 GTCGAC GT^[AC][GT]AC
AccIII 419 TCCGGA T^CCGGA
AfaI 303 GTAC GT^AC
AflIII 959 ACATGT A^C[GA][CT]GT
Alw44I 70 GTGCAC G^TGCAC
AlwI 372 GGATC GGATC(4/5)
AocI 924 CCTCAGG CC^T[ACGT]AGG
ApaLI 70 GTGCAC G^TGCAC
AvaI 1114 CCCGAG C^[CT]CG[GA]G
BanI 801 GGTGCC G^G[CT][GA]CC
BanII 310 GAGCTC G[GA]GC[CT]^C
BbvCI 765 CCTCAGC CCTCAGC(-5/-2)
BclI 509 TGATCA T^GATCA
BsaAI 88 TACGTG [CT]AC^GT[GA]
BsaWI 419 TCCGGA [AT]^CCGG[AT]
BseAI 419 TCCGGA T^CCGGA
BsmFI 758 GGGAC GGGAC(10/14)
BsmI 567 GAATGC GAATGC(1/-1)
BsoBI 1114 CCCGAG C^[CT]CG[GA]G
Bsp1407I 302 TGTACA T^GTACA
BspEI 419 TCCGGA T^CCGGA
BspLU11I 959 ACATGT A^CATGT
BsrDI 777 CATTGC GCAATG(2/0)
BsrGI 302 TGTACA T^GTACA
BssSI 987 CACGAG CACGAG(-5/-1)
BstAPI 1023 GCAGGCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 372 GGATCT [GA]^GATC[CT]
Bsu36I 924 CCTCAGG CC^T[ACGT]AGG
EarI 631 CTCTTC CTCTTC(1/4)
Eco81I 924 CCTCAGG CC^T[ACGT]AGG
FbaI 509 TGATCA T^GATCA
HaeII 1026 GGCGCT [GA]GCGC^[CT]
HgaI 352 GACGC GACGC(5/10)
HincII 919 GTCGAC GT[CT]^[GA]AC
HindII 919 GTCGAC GT[CT]^[GA]AC
Ksp632I 631 CTCTTC CTCTTC(1/4)
MflI 372 GGATCT [GA]^GATC[CT]
MroI 419 TCCGGA T^CCGGA
NcoI 322 CCATGG C^CATGG
PflMI 1191 CCAAGTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 912 GACTGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1030 CTGCAG CTGCA^G
RsaI 303 GTAC GT^AC
SacI 310 GAGCTC GAGCT^C
SalI 919 GTCGAC G^TCGAC
SfaNI 297 GCATC GCATC(5/9)
SfcI 1030 CTGCAG C^T[GA][CT]AG
SfiI 93 GGCCCACCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspBI 302 TGTACA T^GTACA
StuI 143 AGGCCT AGG^CCT
Van91I 1191 CCAAGTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 896 CCAAGTTCTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 372 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccII 354 CGCG CG^CG
1020 CGCG
AlwNI 846 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
1024 CAGGCGCTG
BalI 197 TGGCCA TGG^CCA
720 TGGCCA
BbsI 489 GAAGAC GAAGAC(2/6)
594 GAAGAC
BfaI 28 CTAG C^TAG
937 CTAG
BglI 94 GCCCACCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1102 GCCCGCATGGC
BpuAI 489 GAAGAC GAAGAC(2/6)
594 GAAGAC
BspMI 22 ACCTGC ACCTGC(4/8)
132 GCAGGT
BstUI 354 CGCG CG^CG
1020 CGCG
EcoT14I 149 CCAAGG C^C[AT][AT]GG
322 CCATGG
MaeI 28 CTAG C^TAG
937 CTAG
MluNI 197 TGGCCA TGG^CCA
720 TGGCCA
MscI 197 TGGCCA TGG^CCA
720 TGGCCA
MvnI 354 CGCG CG^CG
1020 CGCG
NspI 770 GCATGT [GA]CATG^[CT]
959 ACATGT
StyI 149 CCAAGG C^C[AT][AT]GG
322 CCATGG
TaqI 920 TCGA T^CGA
1046 TCGA
TthHB8I 920 TCGA T^CGA
1046 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AciI AluI AspHI AvaII BbvI
BmyI BpmI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DpnII DsaI EaeI
EcoRII FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeII MboI MboII
MnlI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV PleI PvuII
Sau3AI Sau96I ScrFI TfiI TseI
TspRI