Restriction map for
pF1KB5647
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# NO CUT ENZYMES ( 136 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AflIII
AgeI Alw44I AlwNI AocI Aor51HI
ApaLI AscI Asp718I AspI AvaII
AviII AvrII BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BfrI
BglII BlnI BpuAI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsgI BsiEI BsiWI BsmBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CfoI ClaI
CpoI DraIII DrdI EagI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRV EcoT22I FauI FbaI FseI
FspI HaeII HhaI Hin1I HinP1I
HpaI KasI KpnI KspI MaeII
MamI MluI MroI MvnI NarI
NdeI NheI NotI NruI NsiI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI StuI
SwaI Tth111I XbaI XcmI XmaCI
XmaI
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# ONE CUT ENZYMES ( 64 enzymes) #
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AhdI 593 GACGAACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 585 GGGCCC GGGCC^C
AseI 913 ATTAAT AT^TAAT
AsnI 913 ATTAAT AT^TAAT
Asp700I 517 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 593 GACGAACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 886 GAGCTC G[AT]GC[AT]^C
AvaI 173 CTCGAG C^[CT]CG[GA]G
BalI 378 TGGCCA TGG^CCA
BamHI 81 GGATCC G^GATCC
BanI 61 GGCACC G^G[CT][GA]CC
BcnI 42 CCCGG CC^[GC]GG
BglI 178 GCCGATGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 862 CTGGAG CTGGAG(16/14)
BsiHKAI 886 GAGCTC G[AT]GC[AT]^C
BsmAI 920 GAGAC GTCTC(1/5)
BsmFI 258 GGGAC GGGAC(10/14)
BsmI 621 GAATGC GAATGC(1/-1)
BsoBI 173 CTCGAG C^[CT]CG[GA]G
BsrFI 217 GCCGGC [GA]^CCGG[CT]
BsrI 933 ACTGG ACTGG(1/-1)
BstAPI 224 GCATTGTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 217 GCCGGC [GA]^CCGG[CT]
DraI 880 TTTAAA TTT^AAA
DraII 585 GGGCCCT [GA]G^G[ACGT]CC[CT]
DsaI 581 CCATGG C^C[GA][CT]GG
EaeI 378 TGGCCA [CT]^GGCC[GA]
Eam1105I 593 GACGAACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 585 GGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRI 829 GAATTC G^AATTC
EcoT14I 581 CCATGG C^C[AT][AT]GG
HgaI 124 GACGC GACGC(5/10)
HincII 646 GTTGAC GT[CT]^[GA]AC
HindII 646 GTTGAC GT[CT]^[GA]AC
HindIII 636 AAGCTT A^AGCTT
HphI 272 TCACC GGTGA(8/7)
MaeIII 122 GTGAC ^GT[ACGT]AC
MfeI 443 CAATTG C^AATTG
MluNI 378 TGGCCA TGG^CCA
MscI 378 TGGCCA TGG^CCA
MslI 576 CACCCCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 76 CAGCTG C[AC]G^C[GT]G
MunI 443 CAATTG C^AATTG
NaeI 217 GCCGGC GCC^GGC
NciI 42 CCCGG CC^[GC]GG
NcoI 581 CCATGG C^CATGG
NgoMIV 217 GCCGGC G^CCGGC
NspI 111 GCATGT [GA]CATG^[CT]
PaeR7I 173 CTCGAG C^TCGAG
PleI 739 GAGTC GAGTC(4/5)
PshBI 913 ATTAAT AT^TAAT
PstI 148 CTGCAG CTGCA^G
PvuII 76 CAGCTG CAG^CTG
SacI 886 GAGCTC GAGCT^C
SfaNI 484 GCATC GCATC(5/9)
SmlI 173 CTCGAG C^T[CT][GA]AG
StyI 581 CCATGG C^C[AT][AT]GG
TaqI 174 TCGA T^CGA
TfiI 134 GATTC G^A[AT]TC
Tsp45I 122 GTGAC ^GT[GC]AC
TspRI 162 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 174 TCGA T^CGA
XhoI 173 CTCGAG C^TCGAG
XmnI 517 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
BanII 585 GGGCCC G[GA]GC[CT]^C
886 GAGCTC
BlpI 546 GCTTAGC GC^T[ACGT]AGC
888 GCTCAGC
BmyI 585 GGGCCC G[AGT]GC[ACT]^C
886 GAGCTC
Bpu1102I 546 GCTTAGC GC^T[ACGT]AGC
888 GCTCAGC
Bsp1286I 585 GGGCCC G[AGT]GC[ACT]^C
886 GAGCTC
BstF5I 924 CATCC GGATG(2/0)
791 GGATG
BstYI 81 GGATCC [GA]^GATC[CT]
144 GGATCT
CelII 546 GCTTAGC GC^T[ACGT]AGC
888 GCTCAGC
DdeI 547 CTTAG C^T[ACGT]AG
889 CTCAG
EarI 265 GAAGAG CTCTTC(1/4)
754 GAAGAG
FokI 924 CATCC GGATG(9/13)
791 GGATG
HapII 43 CCGG C^CGG
218 CCGG
HinfI 134 GATTC G^A[ACGT]TC
739 GAGTC
HpaII 43 CCGG C^CGG
218 CCGG
Ksp632I 265 GAAGAG CTCTTC(1/4)
754 GAAGAG
MflI 81 GGATCC [GA]^GATC[CT]
144 GGATCT
MspI 43 CCGG C^CGG
218 CCGG
NlaIII 112 CATG CATG^
582 CATG
PflMI 526 CCAAAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
927 CCACTTACTGG
SfcI 22 CTACAG C^T[GA][CT]AG
148 CTGCAG
Van91I 526 CCAAAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
927 CCACTTACTGG
XhoII 81 GGATCC [GA]^GATC[CT]
144 GGATCT
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# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
BbvI BfaI BsaJI BsiYI BslI
BssKI BstNI Cac8I Cfr13I DpnII
EcoRII Fnu4HI HaeIII ItaI MaeI
MboI MboII MnlI MseI MvaI
MwoI NdeII NlaIV RsaI Sau3AI
Sau96I ScrFI SspI Tru9I TseI
Tsp509I