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# NO CUT ENZYMES ( 223 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AflII
AflIII AgeI AluI Alw44I AlwI
AlwNI AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspHI AspI AvaI AviII
AvrII BalI BamHI BanI BanII
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BcnI BfaI BfrI
BglI BglII BlnI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsiEI BsiHKAI BsiWI BsmAI BsmBI
BsmFI BsmI BsoBI Bsp1286I Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BsrI
BssHII BssSI Bst1107I BstAPI BstEII
BstF5I BstPI BstUI BstXI BstYI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DdeI DpnII DraI
DraII DraIII DrdI DsaI EaeI
EagI EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FauI FbaI
FokI FseI FspI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HinfI HpaI
HpaII HphI KasI KpnI Ksp632I
KspI MaeI MaeII MamI MboI
MboII MfeI MflI MluI MluNI
MroI MscI MslI MspI MunI
MvnI MwoI NaeI NarI NciI
NcoI NdeI NdeII NgoMIV NheI
NlaIV NotI NruI NsiI PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsaI RsrII SacI SacII
SalI SapI Sau3AI SbfI ScaI
SexAI SfcI SfiI SfoI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI TfiI Tsp509I TspRI Tth111I
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 25 enzymes) #
###################################
AciI 109 GCGG CCGC(-3/-1)
AhdI 112 GACCTCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 112 GACCTCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII 111 GGACC G^G[AT]CC
BbvI 54 GCAGC GCAGC(8/12)
BsaJI 35 CCCAGG C^C[ACGT][ACGT]GG
BsgI 52 GTGCAG GTGCAG(16/14)
BstBI 96 TTCGAA TT^CGAA
Cac8I 16 GCAAGC GC[ACGT]^[ACGT]GC
Cfr13I 111 GGACC G^G[ACGT]CC
Eam1105I 112 GACCTCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Fnu4HI 54 GCAGC GC^[ACGT]GC
ItaI 54 GCAGC GC^[ACGT]GC
MaeIII 146 GTGAC ^GT[ACGT]AC
MseI 61 TTAA T^TAA
MspA1I 107 CAGCGG C[AC]G^C[GT]G
NspI 135 ACATGC [GA]CATG^[CT]
NspV 96 TTCGAA TT^CGAA
Sau96I 111 GGACC G^G[ACGT]CC
SfuI 96 TTCGAA TT^CGAA
TaqI 97 TCGA T^CGA
Tru9I 61 TTAA T^TAA
TseI 54 GCAGC G^C[AT]GC
Tsp45I 146 GTGAC ^GT[GC]AC
TthHB8I 97 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 9 enzymes) #
###################################
BsiYI 35 CCCAGGCAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
183 CCCTTTCAAGG
BslI 35 CCCAGGCAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
183 CCCTTTCAAGG
BssKI 36 CCAGG ^CC[ACGT]GG
140 CCAGG
BstNI 36 CCAGG CC^[AT]GG
140 CCAGG
EcoRII 36 CCAGG ^CC[AT]GG
140 CCAGG
MvaI 36 CCAGG CC^[AT]GG
140 CCAGG
NlaIII 117 CATG CATG^
136 CATG
ScrFI 36 CCAGG CC^[ACGT]GG
140 CCAGG
SfaNI 103 GCATC GCATC(5/9)
206 GCATC
#########################################
# 3 OR MORE CUT ENZYMES ( 1 enzymes) #
#########################################
MnlI