Restriction map for
pF1KB5590
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# NO CUT ENZYMES ( 119 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI AlwI AlwNI
AocI Aor51HI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BalI BamHI BbvCI BcgI
BciVI BclI BfrI BglII BlnI
BlpI BpmI Bpu1102I BsaI BsaWI
BseAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspLU11I BspMI BsrBI
BsrDI BsrGI BssHII Bst1107I BstBI
BstYI BstZ17I Bsu36I CelII ClaI
CpoI DdeI DraI Eam1105I Eco47III
Eco81I EcoRI EcoRV EcoT22I FbaI
FspI HincII HindII HindIII HpaI
HphI KpnI KspI MfeI MflI
MluI MluNI MroI MscI MunI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmeI PpuMI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
SapI ScaI SexAI SfiI SfuI
SgrAI SmlI SnaBI SpeI SspBI
SspI SwaI TaqI Tth111I TthHB8I
XbaI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AcsI 844 AAATTC [GA]^AATT[CT]
AcyI 117 GGCGCC G[GA]^CG[CT]C
AflIII 557 ACACGT A^C[GA][CT]GT
ApaI 18 GGGCCC GGGCC^C
ApoI 844 AAATTC [GA]^AATT[CT]
BbeI 117 GGCGCC GGCGC^C
BbrPI 372 CACGTG CAC^GTG
BfaI 688 CTAG C^TAG
BsaAI 372 CACGTG [CT]AC^GT[GA]
BsaHI 117 GGCGCC G[GA]^CG[CT]C
BsmFI 986 GTCCC GGGAC(10/14)
BspHI 935 TCATGA T^CATGA
BssSI 145 CTCGTG CACGAG(-5/-1)
BstAPI 758 GCAAAAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 660 GGTTACC G^GT[ACGT]ACC
BstPI 660 GGTTACC G^GT[ACGT]ACC
DpnII 273 GATC ^GATC
DraIII 609 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 925 GACGGCGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 518 CCGTGG C^C[GA][CT]GG
EcoO65I 660 GGTTACC G^GT[ACGT]ACC
EcoT14I 506 CCAAGG C^C[AT][AT]GG
FseI 65 GGCCGGCC GGCCGG^CC
Hin1I 117 GGCGCC G[GA]^CG[CT]C
KasI 117 GGCGCC G^GCGCC
MaeI 688 CTAG C^TAG
MboI 273 GATC ^GATC
MseI 749 TTAA T^TAA
MslI 819 CACCAGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 117 GGCGCC GG^CGCC
NdeII 273 GATC ^GATC
NspI 398 GCATGC [GA]CATG^[CT]
PleI 340 GAGTC GAGTC(4/5)
PmaCI 372 CACGTG CAC^GTG
PmlI 372 CACGTG CAC^GTG
PshAI 418 GACGTTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 935 TCATGA T^CATGA
Sau3AI 273 GATC ^GATC
SbfI 680 CCTGCAGG CCTGCA^GG
SfoI 117 GGCGCC GGC^GCC
SphI 398 GCATGC GCATG^C
Sse8387I 680 CCTGCAGG CCTGCA^GG
StuI 718 AGGCCT AGG^CCT
StyI 506 CCAAGG C^C[AT][AT]GG
TfiI 1009 GAATC G^A[AT]TC
Tru9I 749 TTAA T^TAA
Tsp509I 845 AATT ^AATT
TspRI 367 CAGTG CA[GC]TG[ACGT][ACGT]^
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
BanI 101 GGTGCC G^G[CT][GA]CC
117 GGCGCC
BanII 18 GGGCCC G[GA]GC[CT]^C
502 GAGCCC
BbsI 553 GAAGAC GAAGAC(2/6)
828 GAAGAC
BpuAI 553 GAAGAC GAAGAC(2/6)
828 GAAGAC
BsaBI 642 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
951 GATGTTCATC
BsmAI 992 GAGAC GTCTC(1/5)
1037 GAGAC
BstF5I 768 CATCC GGATG(2/0)
641 GGATG
BstXI 182 CCAAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
590 CCAACTGCCTGG
DraII 18 GGGCCCC [GA]G^G[ACGT]CC[CT]
233 AGGGCCT
EarI 238 CTCTTC CTCTTC(1/4)
739 GAAGAG
EcoNI 446 CCTTCCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
677 CCTCCTGCAGG
EcoO109I 18 GGGCCCC [GA]G^G[ACGT]CC[CT]
233 AGGGCCT
FokI 768 CATCC GGATG(9/13)
641 GGATG
HaeII 117 GGCGCC [GA]GCGC^[CT]
619 AGCGCC
HgaI 29 GCGTC GACGC(5/10)
173 GCGTC
HinfI 340 GAGTC G^A[ACGT]TC
1009 GAATC
Ksp632I 238 CTCTTC CTCTTC(1/4)
739 GAAGAG
MamI 642 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
951 GATGTTCATC
PflMI 513 CCAAACCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
608 CCACAGAGTGG
PvuII 187 CAGCTG CAG^CTG
354 CAGCTG
SmaI 469 CCCGGG CCC^GGG
656 CCCGGG
Tsp45I 245 GTGAC ^GT[GC]AC
497 GTGAC
Van91I 513 CCAAACCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
608 CCACAGAGTGG
XcmI 513 CCAAACCGTGGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
896 CCACCCTGCCGGTGG
XmaCI 469 CCCGGG C^CCGGG
656 CCCGGG
XmaI 469 CCCGGG C^CCGGG
656 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AccII AciI AfaI AluI Alw44I
ApaLI AspHI AvaI AvaII BbvI
BcnI BglI BmyI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsoBI Bsp1286I BsrFI BsrI BssKI
BstNI BstUI Cac8I CfoI Cfr10I
Cfr13I EaeI EagI EclXI Eco52I
EcoRII FauI Fnu4HI HaeIII HapII
HhaI HinP1I HpaII ItaI MaeII
MaeIII MboII MnlI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NgoMIV NlaIII NlaIV PstI RsaI
Sau96I ScrFI SfaNI SfcI TseI