Restriction map for
pF1KB5582
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# NO CUT ENZYMES ( 150 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AflII AflIII AhdI Alw44I AlwNI
Aor51HI ApaI ApaLI AscI Asp700I
AspEI AvaI AvaII AviII AvrII
BalI BamHI BanII BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BsaI BsaJI BseAI BsgI BsiEI
BsiWI BsmAI BsmBI BsoBI Bsp1407I
BspDI BspEI BspLU11I BspMI BsrBI
BsrGI BssHII BssSI Bst1107I BstAPI
BstEII BstPI BstUI BstXI BstZ17I
Cac8I CelII CfoI ClaI CpoI
DraI DraII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO109I EcoO65I EcoRI EcoT14I
FbaI FseI FspI HaeII HhaI
HinP1I HincII HindII HindIII HpaI
KasI KspI MaeII MamI MfeI
MluI MluNI MroI MscI MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI PacI
PaeR7I PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PvuI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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Acc65I 376 GGTACC G^GTACC
AcyI 859 GGCGTC G[GA]^CG[CT]C
AfaI 377 GTAC GT^AC
AgeI 532 ACCGGT A^CCGGT
AseI 363 ATTAAT AT^TAAT
AsnI 363 ATTAAT AT^TAAT
Asp718I 376 GGTACC G^GTACC
AspHI 757 GTGCTC G[AT]GC[AT]^C
AspI 259 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BcnI 650 CCCGG CC^[GC]GG
BfaI 514 CTAG C^TAG
BmyI 757 GTGCTC G[AGT]GC[ACT]^C
BsaHI 859 GGCGTC G[GA]^CG[CT]C
BsaWI 532 ACCGGT [AT]^CCGG[AT]
BsiHKAI 757 GTGCTC G[AT]GC[AT]^C
Bsp1286I 757 GTGCTC G[AGT]GC[ACT]^C
BsrDI 117 CATTGC GCAATG(2/0)
BsrFI 532 ACCGGT [GA]^CCGG[CT]
BsrI 184 CCAGT ACTGG(1/-1)
BstYI 480 AGATCC [GA]^GATC[CT]
Cfr10I 532 ACCGGT [GA]^CCGG[CT]
Cfr13I 692 GGGCC G^G[ACGT]CC
DraIII 284 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 966 GAAGAG CTCTTC(1/4)
EcoRV 460 GATATC GAT^ATC
EcoT22I 1084 ATGCAT ATGCA^T
FauI 253 GCGGG CCCGC(4/6)
HaeIII 693 GGCC GG^CC
Hin1I 859 GGCGTC G[GA]^CG[CT]C
KpnI 376 GGTACC GGTAC^C
Ksp632I 966 GAAGAG CTCTTC(1/4)
MaeI 514 CTAG C^TAG
MflI 480 AGATCC [GA]^GATC[CT]
MspA1I 919 CAGCTG C[AC]G^C[GT]G
NciI 650 CCCGG CC^[GC]GG
NsiI 1084 ATGCAT ATGCA^T
NspI 841 GCATGT [GA]CATG^[CT]
PflFI 259 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 283 CCACAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 532 ACCGGT A^CCGGT
PshBI 363 ATTAAT AT^TAAT
PstI 29 CTGCAG CTGCA^G
PvuII 919 CAGCTG CAG^CTG
RsaI 377 GTAC GT^AC
Sau96I 692 GGGCC G^G[ACGT]CC
SfcI 29 CTGCAG C^T[GA][CT]AG
TfiI 107 GAATC G^A[AT]TC
Tth111I 259 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 283 CCACAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 480 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AciI 127 GCGG CCGC(-3/-1)
253 GCGG
AocI 169 CCTGAGG CC^T[ACGT]AGG
727 CCTCAGG
BanI 81 GGCACC G^G[CT][GA]CC
376 GGTACC
BsiYI 283 CCACAGTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
984 CCCAAAGTTGG
BslI 283 CCACAGTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
984 CCCAAAGTTGG
BsmFI 195 GGGAC GGGAC(10/14)
982 GTCCC
BspHI 96 TCATGA T^CATGA
821 TCATGA
BstBI 104 TTCGAA TT^CGAA
1103 TTCGAA
BstNI 571 CCTGG CC^[AT]GG
750 CCAGG
Bsu36I 169 CCTGAGG CC^T[ACGT]AGG
727 CCTCAGG
Eco81I 169 CCTGAGG CC^T[ACGT]AGG
727 CCTCAGG
EcoRII 571 CCTGG ^CC[AT]GG
750 CCAGG
HapII 533 CCGG C^CGG
651 CCGG
HgaI 860 GCGTC GACGC(5/10)
866 GCGTC
HpaII 533 CCGG C^CGG
651 CCGG
MseI 364 TTAA T^TAA
722 TTAA
MspI 533 CCGG C^CGG
651 CCGG
MvaI 571 CCTGG CC^[AT]GG
750 CCAGG
NspV 104 TTCGAA TT^CGAA
1103 TTCGAA
RcaI 96 TCATGA T^CATGA
821 TCATGA
SfaNI 142 GATGC GCATC(5/9)
1083 GATGC
SfuI 104 TTCGAA TT^CGAA
1103 TTCGAA
SmlI 350 CTCAAG C^T[CT][GA]AG
835 CTTGAG
TaqI 105 TCGA T^CGA
1104 TCGA
Tru9I 364 TTAA T^TAA
722 TTAA
TthHB8I 105 TCGA T^CGA
1104 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AcsI AluI AlwI ApoI BbvI
BseRI BsmI BssKI BstF5I DdeI
DpnII Fnu4HI FokI HinfI HphI
ItaI MaeIII MboI MboII MnlI
MslI MwoI NdeII NlaIII NlaIV
PleI Sau3AI ScrFI TseI Tsp45I
Tsp509I TspRI