Restriction map for
pF1KB5569
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# NO CUT ENZYMES ( 145 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AvaI AvrII BanI BbeI
BbrPI BbsI BbvCI BcgI BclI
BfrI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BseAI
BseRI BsgI BsiEI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspEI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
CelII Cfr10I CpoI DraII DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO109I EcoO65I
EcoT22I FauI FbaI FseI HaeII
HgaI Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MamI
MfeI MluI MroI MspA1I MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RsrII SacII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AlwNI 236 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 405 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 937 GAGCTC G[AT]GC[AT]^C
AspI 94 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 908 TGCGCA TGC^GCA
BamHI 1221 GGATCC G^GATCC
BanII 937 GAGCTC G[GA]GC[CT]^C
BciVI 1075 GGATAC GTATCC(6/5)
BcnI 927 CCCGG CC^[GC]GG
BglI 972 GCCAAATGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 937 GAGCTC G[AGT]GC[ACT]^C
BsaI 453 GGTCTC GGTCTC(1/5)
BsaWI 1019 TCCGGT [AT]^CCGG[AT]
BsiHKAI 937 GAGCTC G[AT]GC[AT]^C
Bsp1286I 937 GAGCTC G[AGT]GC[ACT]^C
BspDI 1129 ATCGAT AT^CGAT
BstXI 663 CCATTCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1221 GGATCC [GA]^GATC[CT]
Cac8I 741 GCTAGC GC[ACGT]^[ACGT]GC
CfoI 909 GCGC GCG^C
ClaI 1129 ATCGAT AT^CGAT
DraI 889 TTTAAA TTT^AAA
EarI 1353 GAAGAG CTCTTC(1/4)
EcoNI 303 CCTCACTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 205 GAATTC G^AATTC
FspI 908 TGCGCA TGC^GCA
HhaI 909 GCGC GCG^C
HinP1I 909 GCGC G^CGC
Ksp632I 1353 GAAGAG CTCTTC(1/4)
MaeII 783 ACGT A^CGT
MflI 1221 GGATCC [GA]^GATC[CT]
MseI 890 TTAA T^TAA
NciI 927 CCCGG CC^[GC]GG
NheI 741 GCTAGC G^CTAGC
NspI 905 ACATGC [GA]CATG^[CT]
PflFI 94 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 565 CCAGGTATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 1395 CTGCAG CTGCA^G
SacI 937 GAGCTC GAGCT^C
ScaI 1040 AGTACT AGT^ACT
TaqI 1130 TCGA T^CGA
Tru9I 890 TTAA T^TAA
Tth111I 94 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1130 TCGA T^CGA
Van91I 565 CCAGGTATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 69 TCTAGA T^CTAGA
XcmI 768 CCAAGCCATATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1221 GGATCC [GA]^GATC[CT]
XmnI 405 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AciI 159 GCGG CCGC(-3/-1)
646 GCGG
BalI 961 TGGCCA TGG^CCA
1266 TGGCCA
BsmAI 48 GTCTC GTCTC(1/5)
454 GTCTC
BsmFI 506 GGGAC GGGAC(10/14)
1056 GGGAC
EcoRV 480 GATATC GAT^ATC
691 GATATC
EcoT14I 449 CCAAGG C^C[AT][AT]GG
1082 CCAAGG
HapII 928 CCGG C^CGG
1020 CCGG
HpaII 928 CCGG C^CGG
1020 CCGG
MluNI 961 TGGCCA TGG^CCA
1266 TGGCCA
MscI 961 TGGCCA TGG^CCA
1266 TGGCCA
MslI 586 CATTTTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1126 CATATCGATG
MspI 928 CCGG C^CGG
1020 CCGG
NlaIV 314 GGAACC GG[ACGT]^[ACGT]CC
1221 GGATCC
StyI 449 CCAAGG C^C[AT][AT]GG
1082 CCAAGG
TfiI 867 GATTC G^A[AT]TC
899 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
AvaII BbvI BfaI BpmI BsaJI
BsiYI BslI BspHI BsrI BssKI
BstF5I BstNI Cfr13I DdeI DpnII
EaeI EcoRII Fnu4HI FokI HaeIII
HinfI HphI ItaI MaeI MaeIII
MboI MboII MnlI MvaI MwoI
NdeII NlaIII PleI RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
TseI Tsp45I Tsp509I TspRI