Restriction map for
pF1KB5529
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# NO CUT ENZYMES ( 125 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AocI
Aor51HI ApoI AscI AseI AsnI
Asp700I Asp718I AspI AviII BbrPI
BbsI BcgI BciVI BclI BfrI
BglII BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaI BsaWI BseAI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrGI
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraIII DrdI EagI EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI KpnI
KspI MamI MfeI MluI MroI
MseI MunI NheI NotI NruI
NsiI NspI NspV PacI PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
ScaI SexAI SfuI SmaI SnaBI
SpeI SphI SspBI SspI StuI
SwaI TfiI Tru9I Tsp509I Tth111I
Van91I XbaI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AfaI 815 GTAC GT^AC
AhdI 121 GACTTCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 322 GTGCAC G^TGCAC
ApaLI 322 GTGCAC G^TGCAC
AspEI 121 GACTTCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 753 CCTAGG C^CTAGG
BamHI 402 GGATCC G^GATCC
BanI 75 GGCGCC G^G[CT][GA]CC
BbeI 75 GGCGCC GGCGC^C
BlnI 753 CCTAGG C^CTAGG
BseRI 590 GAGGAG GAGGAG(10/8)
BsmAI 336 GAGAC GTCTC(1/5)
BspMI 825 GCAGGT ACCTGC(4/8)
BssHII 276 GCGCGC G^CGCGC
BstAPI 378 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 402 GGATCC [GA]^GATC[CT]
Eam1105I 121 GACTTCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 666 CCTGCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HinfI 346 GACTC G^A[ACGT]TC
KasI 75 GGCGCC G^GCGCC
MaeIII 838 GTGAC ^GT[ACGT]AC
MflI 402 GGATCC [GA]^GATC[CT]
MslI 723 CATCCATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 266 GCCGGC GCC^GGC
NarI 75 GGCGCC GG^CGCC
NcoI 492 CCATGG C^CATGG
NdeI 727 CATATG CA^TATG
NgoMIV 266 GCCGGC G^CCGGC
PaeR7I 48 CTCGAG C^TCGAG
PleI 346 GACTC GAGTC(4/5)
RsaI 815 GTAC GT^AC
SacI 51 GAGCTC GAGCT^C
SbfI 666 CCTGCAGG CCTGCA^GG
SfiI 154 GGCCTCTCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 75 GGCGCC GGC^GCC
SgrAI 292 CACCGGCG C[GA]^CCGG[CT]G
SmlI 48 CTCGAG C^T[CT][GA]AG
Sse8387I 666 CCTGCAGG CCTGCA^GG
TaqI 49 TCGA T^CGA
Tsp45I 838 GTGAC ^GT[GC]AC
TthHB8I 49 TCGA T^CGA
XcmI 818 CCAAAGAGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 48 CTCGAG C^TCGAG
XhoII 402 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 26 enzymes) #
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AcyI 75 GGCGCC G[GA]^CG[CT]C
168 GGCGTC
AlwNI 379 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
382 CAGCTGCTG
AspHI 51 GAGCTC G[AT]GC[AT]^C
322 GTGCAC
AvaII 489 GGACC G^G[AT]CC
615 GGACC
BalI 644 TGGCCA TGG^CCA
851 TGGCCA
BcnI 14 CCGGG CC^[GC]GG
165 CCGGG
BfaI 691 CTAG C^TAG
754 CTAG
BglI 155 GCCTCTCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
288 GCCCCACCGGC
BpmI 706 CTGGAG CTGGAG(16/14)
745 CTGGAG
BsaHI 75 GGCGCC G[GA]^CG[CT]C
168 GGCGTC
BsiHKAI 51 GAGCTC G[AT]GC[AT]^C
322 GTGCAC
BsmFI 17 GGGAC GGGAC(10/14)
344 GGGAC
BsrDI 455 GCAATG GCAATG(2/0)
461 GCAATG
BsrFI 266 GCCGGC [GA]^CCGG[CT]
293 ACCGGC
BstXI 359 CCAATGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
510 CCAGCGAGATGG
Cfr10I 266 GCCGGC [GA]^CCGG[CT]
293 ACCGGC
DsaI 308 CCACGG C^C[GA][CT]GG
492 CCATGG
EcoT14I 492 CCATGG C^C[AT][AT]GG
753 CCTAGG
HaeII 75 GGCGCC [GA]GCGC^[CT]
749 AGCGCC
Hin1I 75 GGCGCC G[GA]^CG[CT]C
168 GGCGTC
MaeI 691 CTAG C^TAG
754 CTAG
MaeII 206 ACGT A^CGT
563 ACGT
MluNI 644 TGGCCA TGG^CCA
851 TGGCCA
MscI 644 TGGCCA TGG^CCA
851 TGGCCA
NciI 14 CCGGG CC^[GC]GG
165 CCGGG
StyI 492 CCATGG C^C[AT][AT]GG
753 CCTAGG
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# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AccII AciI AluI AlwI ApaI
AvaI BanII BbvCI BbvI BmyI
BsaJI BsgI BsiYI BslI BsoBI
Bsp1286I BsrI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DpnII DraII EaeI EarI EcoO109I
EcoRII FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HpaII
HphI ItaI Ksp632I MboI MboII
MnlI MspA1I MspI MvaI MvnI
MwoI NdeII NlaIII NlaIV PstI
PvuII Sau3AI Sau96I ScrFI SfaNI
SfcI TseI TspRI