Restriction map for
pF1KB5497
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# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AflIII
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AviII AvrII BalI
BamHI BanI BbeI BbrPI BbsI
BbvCI BcgI BfrI BglI BglII
BlnI BpuAI BsaAI BsaBI BsaHI
BsaWI BseAI BsiEI BsiWI BsmBI
BsmFI Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I Cfr10I ClaI
CpoI DraI DraIII DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoT22I FauI FseI FspI
HaeII HgaI Hin1I HpaI KasI
KpnI KspI MamI MfeI MluI
MluNI MroI MscI MunI MvnI
NaeI NarI NgoMIV NheI NotI
NruI NsiI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tth111I XcmI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AocI 310 CCTAAGG CC^T[ACGT]AGG
Asp700I 1287 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BciVI 1022 GTATCC GTATCC(6/5)
BclI 299 TGATCA T^GATCA
BfaI 833 CTAG C^TAG
BlpI 1315 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1315 GCTCAGC GC^T[ACGT]AGC
BsgI 331 GTGCAG GTGCAG(16/14)
BsmI 966 GAATGC GAATGC(1/-1)
BspHI 855 TCATGA T^CATGA
BspMI 492 GCAGGT ACCTGC(4/8)
BsrDI 938 GCAATG GCAATG(2/0)
Bsu36I 310 CCTAAGG CC^T[ACGT]AGG
Cac8I 970 GCAGGC GC[ACGT]^[ACGT]GC
CelII 1315 GCTCAGC GC^T[ACGT]AGC
CfoI 1047 GCGC GCG^C
DraII 189 AGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 626 CCATGG C^C[GA][CT]GG
Eco81I 310 CCTAAGG CC^T[ACGT]AGG
EcoO109I 189 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRV 1412 GATATC GAT^ATC
FbaI 299 TGATCA T^GATCA
HhaI 1047 GCGC GCG^C
HinP1I 1047 GCGC G^CGC
HincII 227 GTCAAC GT[CT]^[GA]AC
HindII 227 GTCAAC GT[CT]^[GA]AC
MaeI 833 CTAG C^TAG
MseI 1061 TTAA T^TAA
MspA1I 388 CAGCTG C[AC]G^C[GT]G
NcoI 626 CCATGG C^CATGG
NdeI 1278 CATATG CA^TATG
PaeR7I 194 CTCGAG C^TCGAG
PpuMI 189 AGGACCT [GA]G^G[AT]CC[CT]
PvuII 388 CAGCTG CAG^CTG
RcaI 855 TCATGA T^CATGA
SfcI 238 CTACAG C^T[GA][CT]AG
SmaI 326 CCCGGG CCC^GGG
SmlI 194 CTCGAG C^T[CT][GA]AG
Tru9I 1061 TTAA T^TAA
Tsp45I 1169 GTCAC ^GT[GC]AC
XbaI 832 TCTAGA T^CTAGA
XhoI 194 CTCGAG C^TCGAG
XmaCI 326 CCCGGG C^CCGGG
XmaI 326 CCCGGG C^CCGGG
XmnI 1287 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AfaI 481 GTAC GT^AC
755 GTAC
AlwNI 265 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
292 CAGTTTCTG
AspHI 220 GAGCTC G[AT]GC[AT]^C
316 GAGCTC
BsaI 181 GAGACC GGTCTC(1/5)
247 GAGACC
BsiHKAI 220 GAGCTC G[AT]GC[AT]^C
316 GAGCTC
BstF5I 303 CATCC GGATG(2/0)
723 CATCC
BstYI 266 AGATCC [GA]^GATC[CT]
393 GGATCT
EarI 178 GAAGAG CTCTTC(1/4)
593 GAAGAG
FokI 303 CATCC GGATG(9/13)
723 CATCC
HapII 216 CCGG C^CGG
327 CCGG
HindIII 565 AAGCTT A^AGCTT
1205 AAGCTT
HpaII 216 CCGG C^CGG
327 CCGG
Ksp632I 178 GAAGAG CTCTTC(1/4)
593 GAAGAG
MaeII 362 ACGT A^CGT
708 ACGT
MflI 266 AGATCC [GA]^GATC[CT]
393 GGATCT
MspI 216 CCGG C^CGG
327 CCGG
NspI 747 ACATGC [GA]CATG^[CT]
1320 GCATGT
PflMI 24 CCATTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
726 CCAGCACTTGG
RsaI 481 GTAC GT^AC
755 GTAC
SacI 220 GAGCTC GAGCT^C
316 GAGCTC
SfaNI 339 GCATC GCATC(5/9)
663 GCATC
Van91I 24 CCATTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
726 CCAGCACTTGG
XhoII 266 AGATCC [GA]^GATC[CT]
393 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AcsI AluI AlwI ApoI AvaI
AvaII BanII BbvI BcnI BmyI
BpmI BsaJI BseRI BsiYI BslI
BsmAI BsoBI Bsp1286I BsrI BssKI
BstNI Cfr13I DdeI DpnII EcoNI
EcoRI EcoRII EcoT14I Fnu4HI HaeIII
HinfI HphI ItaI MaeIII MboI
MboII MnlI MslI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
Sau3AI Sau96I ScrFI StuI StyI
TaqI TfiI TseI Tsp509I TspRI
TthHB8I