Restriction map for
pF1KB5486
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# NO CUT ENZYMES ( 159 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BalI BamHI BanII BbeI BbrPI
BbvCI BcgI BciVI BclI BfaI
BfrI BlnI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsiEI
BsiWI BsmAI BsmBI BsmFI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRV
EcoT22I FbaI FseI FspI HaeII
Hin1I HincII HindII HindIII HpaI
KasI KpnI Ksp632I KspI MaeI
MamI MfeI MluI MluNI MroI
MscI MseI MslI MspA1I MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TfiI Tru9I Tth111I Van91I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AccII 278 CGCG CG^CG
AcsI 502 GAATTC [GA]^AATT[CT]
AlwNI 40 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 502 GAATTC [GA]^AATT[CT]
AspHI 196 GAGCAC G[AT]GC[AT]^C
AvaI 28 CCCGAG C^[CT]CG[GA]G
AvaII 42 GGACC G^G[AT]CC
BbsI 202 GTCTTC GAAGAC(2/6)
BglI 113 GCCCCACCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 264 GCTGAGC GC^T[ACGT]AGC
BmyI 196 GAGCAC G[AGT]GC[ACT]^C
Bpu1102I 264 GCTGAGC GC^T[ACGT]AGC
BpuAI 202 GTCTTC GAAGAC(2/6)
BsgI 373 GTGCAG GTGCAG(16/14)
BsiHKAI 196 GAGCAC G[AT]GC[AT]^C
BsiYI 126 CCTGACCGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 126 CCTGACCGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsoBI 28 CCCGAG C^[CT]CG[GA]G
Bsp1286I 196 GAGCAC G[AGT]GC[ACT]^C
BspMI 44 ACCTGC ACCTGC(4/8)
BsrDI 258 CATTGC GCAATG(2/0)
BsrFI 118 ACCGGC [GA]^CCGG[CT]
BsrI 569 CCAGT ACTGG(1/-1)
BssSI 73 CACGAG CACGAG(-5/-1)
BstAPI 39 GCAGGACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 420 GGATG GGATG(2/0)
BstNI 327 CCTGG CC^[AT]GG
BstUI 278 CGCG CG^CG
CelII 264 GCTGAGC GC^T[ACGT]AGC
Cfr10I 118 ACCGGC [GA]^CCGG[CT]
DdeI 265 CTGAG C^T[ACGT]AG
DraII 41 AGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 131 CCGTGG C^C[GA][CT]GG
EaeI 120 CGGCCA [CT]^GGCC[GA]
EcoO109I 41 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 502 GAATTC G^AATTC
EcoRII 327 CCTGG ^CC[AT]GG
EcoT14I 494 CCTTGG C^C[AT][AT]GG
FokI 420 GGATG GGATG(9/13)
HgaI 178 GACGC GACGC(5/10)
HinfI 424 GAGTC G^A[ACGT]TC
HphI 65 TCACC GGTGA(8/7)
MaeII 200 ACGT A^CGT
MaeIII 271 GTGAC ^GT[ACGT]AC
MvaI 327 CCTGG CC^[AT]GG
MvnI 278 CGCG CG^CG
NlaIII 318 CATG CATG^
NspI 317 GCATGT [GA]CATG^[CT]
PleI 424 GAGTC GAGTC(4/5)
PpuMI 41 AGGACCT [GA]G^G[AT]CC[CT]
StyI 494 CCTTGG C^C[AT][AT]GG
Tsp45I 271 GTGAC ^GT[GC]AC
Tsp509I 503 AATT ^AATT
XcmI 380 CCATCTACAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 20 enzymes) #
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AfaI 90 GTAC GT^AC
321 GTAC
BanI 232 GGCACC G^G[CT][GA]CC
514 GGTGCC
BcnI 246 CCGGG CC^[GC]GG
432 CCCGG
BglII 149 AGATCT A^GATCT
452 AGATCT
BpmI 289 CTGGAG CTGGAG(16/14)
364 CTGGAG
BstYI 149 AGATCT [GA]^GATC[CT]
452 AGATCT
CfoI 262 GCGC GCG^C
441 GCGC
FauI 48 GCGGG CCCGC(4/6)
279 GCGGG
HhaI 262 GCGC GCG^C
441 GCGC
HinP1I 262 GCGC G^CGC
441 GCGC
MflI 149 AGATCT [GA]^GATC[CT]
452 AGATCT
NciI 246 CCGGG CC^[GC]GG
432 CCCGG
NlaIV 232 GGCACC GG[ACGT]^[ACGT]CC
514 GGTGCC
RsaI 90 GTAC GT^AC
321 GTAC
SfaNI 360 GATGC GCATC(5/9)
411 GATGC
SmlI 103 CTCAAG C^T[CT][GA]AG
301 CTCAAG
TaqI 215 TCGA T^CGA
239 TCGA
TspRI 550 CAGTG CA[GC]TG[ACGT][ACGT]^
564 CAGTG
TthHB8I 215 TCGA T^CGA
239 TCGA
XhoII 149 AGATCT [GA]^GATC[CT]
452 AGATCT
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# 3 OR MORE CUT ENZYMES ( 25 enzymes) #
#########################################
AciI AluI BbvI BsaJI BssKI
Cac8I Cfr13I DpnII Fnu4HI HaeIII
HapII HpaII ItaI MboI MboII
MnlI MspI MwoI NdeII PstI
Sau3AI Sau96I ScrFI SfcI TseI