Restriction map for
pF1KB5477
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# NO CUT ENZYMES ( 131 enzymes) #
##################################
AatII Acc65I AccI AcsI AcyI
AflII AgeI Alw44I Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspHI AspI AvrII
BalI BbeI BbrPI BcgI BciVI
BclI BfrI BglI BglII BlnI
BsaAI BsaBI BsaHI BsaI BsgI
BsiHKAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspHI BspMI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstXI BstZ17I Cfr10I
ClaI CpoI DraI DraIII EagI
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FseI
Hin1I HincII HindII HpaI KasI
KpnI KspI MamI MluI MluNI
MscI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
PstI RcaI RsrII SacI SacII
SalI SbfI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI TfiI Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AccII 458 CGCG CG^CG
AccIII 202 TCCGGA T^CCGGA
AclI 647 AACGTT AA^CGTT
AflIII 1058 ACATGT A^C[GA][CT]GT
AhdI 444 GACCAACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 310 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 262 CCTGAGG CC^T[ACGT]AGG
AspEI 444 GACCAACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 368 TGCGCA TGC^GCA
BbvCI 700 GCTGAGG CCTCAGC(-5/-2)
BfaI 472 CTAG C^TAG
BlpI 97 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 97 GCTGAGC GC^T[ACGT]AGC
BseAI 202 TCCGGA T^CCGGA
BsiEI 1044 CGATCG CG[GA][CT]^CG
BsmAI 1067 GTCTC GTCTC(1/5)
BspEI 202 TCCGGA T^CCGGA
BspLU11I 1058 ACATGT A^CATGT
BsrBI 897 CCGCTC CCGCTC(-3/-3)
BsrI 1020 CCAGT ACTGG(1/-1)
BstUI 458 CGCG CG^CG
Bsu36I 262 CCTGAGG CC^T[ACGT]AGG
CelII 97 GCTGAGC GC^T[ACGT]AGC
DraII 783 GGGTCCT [GA]G^G[ACGT]CC[CT]
DrdI 179 GACACCGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 59 CCATGG C^C[GA][CT]GG
EaeI 894 TGGCCG [CT]^GGCC[GA]
Eam1105I 444 GACCAACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1027 CTCTTC CTCTTC(1/4)
Eco81I 262 CCTGAGG CC^T[ACGT]AGG
EcoNI 315 CCTGAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 783 GGGTCCT [GA]G^G[ACGT]CC[CT]
FspI 368 TGCGCA TGC^GCA
HaeII 230 GGCGCT [GA]GCGC^[CT]
HgaI 534 GCGTC GACGC(5/10)
HindIII 1142 AAGCTT A^AGCTT
HphI 1070 TCACC GGTGA(8/7)
Ksp632I 1027 CTCTTC CTCTTC(1/4)
MaeI 472 CTAG C^TAG
MaeII 648 ACGT A^CGT
MfeI 153 CAATTG C^AATTG
MroI 202 TCCGGA T^CCGGA
MslI 162 CATCTGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 153 CAATTG C^AATTG
MvnI 458 CGCG CG^CG
NcoI 59 CCATGG C^CATGG
NspI 1058 ACATGT [GA]CATG^[CT]
PpuMI 783 GGGTCCT [GA]G^G[AT]CC[CT]
Psp1406I 647 AACGTT AA^CGTT
PvuI 1044 CGATCG CGAT^CG
PvuII 208 CAGCTG CAG^CTG
SapI 1026 GCTCTTC GCTCTTC(1/4)
ScaI 44 AGTACT AGT^ACT
StuI 666 AGGCCT AGG^CCT
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# TWO CUT ENZYMES ( 12 enzymes) #
###################################
AvaI 407 CCCGAG C^[CT]CG[GA]G
865 CCCGAG
BanI 394 GGTGCC G^G[CT][GA]CC
1000 GGCACC
BanII 251 GAGCCC G[GA]GC[CT]^C
1087 GAGCCC
BbvI 104 GCAGC GCAGC(8/12)
1109 GCTGC
BpmI 382 CTGGAG CTGGAG(16/14)
604 CTGGAG
BsaWI 136 ACCGGA [AT]^CCGG[AT]
202 TCCGGA
BsmFI 833 GGGAC GGGAC(10/14)
858 GGGAC
BsoBI 407 CCCGAG C^[CT]CG[GA]G
865 CCCGAG
MseI 293 TTAA T^TAA
845 TTAA
MspA1I 208 CAGCTG C[AC]G^C[GT]G
960 CCGCTG
Tru9I 293 TTAA T^TAA
845 TTAA
TseI 104 GCAGC G^C[AT]GC
1109 GCTGC
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# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BamHI BbsI BcnI BmyI BpuAI
BsaJI BseRI BsiYI BslI Bsp1286I
BssKI BstAPI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
ItaI MaeIII MboI MboII MflI
MnlI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI SfaNI StyI
TaqI Tsp45I Tsp509I TspRI TthHB8I
XhoII