##################################
# NO CUT ENZYMES (  191 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AfaI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwI        
AlwNI       Aor51HI     ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AviII       AvrII       BalI        
BamHI       BbeI        BbrPI       BbsI        BbvCI       
BcgI        BciVI       BclI        BfaI        BfrI        
BglII       BlnI        BlpI        BpmI        Bpu1102I    
BpuAI       BsaAI       BsaBI       BsaHI       BsaI        
BsaWI       BseAI       BseRI       BsgI        BsiEI       
BsiHKAI     BsiWI       BsmAI       BsmBI       BsmFI       
BsmI        Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrDI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstXI       BstYI       
BstZ17I     CelII       CfoI        Cfr10I      ClaI        
CpoI        DraI        DraIII      EaeI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
EcoNI       EcoO65I     EcoRI       EcoRV       EcoT14I     
EcoT22I     FbaI        FseI        FspI        HaeII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HpaI        KasI        KpnI        
Ksp632I     KspI        MaeI        MaeII       MamI        
MfeI        MflI        MluI        MluNI       MroI        
MscI        MseI        MslI        MspA1I      MunI        
MvnI        NaeI        NarI        NcoI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PleI        PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PstI        PvuI        
PvuII       RcaI        RsaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfcI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        StyI        
SwaI        Tru9I       Tsp509I     Tth111I     XbaI        
XcmI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   32 enzymes) #
###################################

AluI          260  AGCT                 AG^CT
AocI           55  CCTCAGG              CC^T[ACGT]AGG
ApaI          332  GGGCCC               GGGCC^C
AvaI          363  CTCGGG               C^[CT]CG[GA]G
BanI            3  GGCACC               G^G[CT][GA]CC
BcnI          291  CCGGG                CC^[GC]GG
BglI          334  GCCCAGTCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsoBI         363  CTCGGG               C^[CT]CG[GA]G
BsrBI         360  CCGCTC               CCGCTC(-3/-3)
Bsu36I         55  CCTCAGG              CC^T[ACGT]AGG
Cac8I         358  GCCCGC               GC[ACGT]^[ACGT]GC
DraII         331  GGGGCCC              [GA]G^G[ACGT]CC[CT]
DrdI          115  GACACTTTTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI          438  CCGTGG               C^C[GA][CT]GG
Eco81I         55  CCTCAGG              CC^T[ACGT]AGG
EcoO109I      331  GGGGCCC              [GA]G^G[ACGT]CC[CT]
FauI          359  CCCGC                CCCGC(4/6)
HapII         291  CCGG                 C^CGG
HinfI         202  GAATC                G^A[ACGT]TC
HpaII         291  CCGG                 C^CGG
MspI          291  CCGG                 C^CGG
NciI          291  CCGGG                CC^[GC]GG
NdeI          212  CATATG               CA^TATG
NlaIII        127  CATG                 CATG^
PflMI         234  CCAGGATGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SfaNI         103  GCATC                GCATC(5/9)
SfiI          333  GGCCCAGTCGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
TaqI            9  TCGA                 T^CGA
TfiI          202  GAATC                G^A[AT]TC
TspRI         132  CACTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I         9  TCGA                 T^CGA
Van91I        234  CCAGGATGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

BanII         332  GGGCCC               G[GA]GC[CT]^C
              350  GAGCCC              
BbvI          217  GCTGC                GCAGC(8/12)
              229  GCTGC               
BmyI          332  GGGCCC               G[AGT]GC[ACT]^C
              350  GAGCCC              
Bsp1286I      332  GGGCCC               G[AGT]GC[ACT]^C
              350  GAGCCC              
BsrI          169  ACTGG                ACTGG(1/-1)
              336  CCAGT               
DdeI           56  CTCAG                C^T[ACGT]AG
              346  CTCAG               
DpnII         136  GATC                 ^GATC
              375  GATC                
Fnu4HI        217  GCTGC                GC^[ACGT]GC
              229  GCTGC               
ItaI          217  GCTGC                GC^[ACGT]GC
              229  GCTGC               
MboI          136  GATC                 ^GATC
              375  GATC                
MboII          31  GAAGA                GAAGA(8/7)
              372  GAAGA               
NdeII         136  GATC                 ^GATC
              375  GATC                
Sau3AI        136  GATC                 ^GATC
              375  GATC                
TseI          217  GCTGC                G^C[AT]GC
              229  GCTGC               


#########################################
# 3 OR MORE CUT ENZYMES (   21 enzymes) #
#########################################

AciI        AvaII       BsaJI       BsiYI       BslI        
BssKI       BstF5I      BstNI       Cfr13I      EcoRII      
FokI        HaeIII      HphI        MaeIII      MnlI        
MvaI        MwoI        NlaIV       Sau96I      ScrFI       
Tsp45I