Restriction map for
pF1KB5420
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# NO CUT ENZYMES ( 129 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AlwNI
AocI ApaI ApoI AscI AseI
AsnI Asp700I Asp718I AvaI AvrII
BamHI BbeI BbrPI BbvCI BcgI
BciVI BclI BfrI BglI BlnI
BlpI Bpu1102I BsaAI BsaBI BsaWI
BseAI BsiEI BsiWI BsmBI BsmI
BsoBI BspDI BspEI BspLU11I BsrBI
BsrDI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraIII
DrdI DsaI EagI EclXI Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI HindIII HpaI
KasI KpnI KspI MamI MfeI
MluI MroI MseI MunI NarI
NcoI NdeI NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI RsrII
SacII SalI SapI SbfI ScaI
SfcI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI SwaI Tru9I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccII 43 CGCG CG^CG
AcyI 1096 GGCGTC G[GA]^CG[CT]C
AhdI 778 GACTTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1164 GTGCAC G^TGCAC
Aor51HI 1205 AGCGCT AGC^GCT
ApaLI 1164 GTGCAC G^TGCAC
AspEI 778 GACTTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1050 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 381 GGTCC G^G[AT]CC
AviII 545 TGCGCA TGC^GCA
BanII 142 GAGCTC G[GA]GC[CT]^C
BbsI 681 GAAGAC GAAGAC(2/6)
BfaI 139 CTAG C^TAG
BglII 29 AGATCT A^GATCT
BpuAI 681 GAAGAC GAAGAC(2/6)
BsaHI 1096 GGCGTC G[GA]^CG[CT]C
BsaI 85 GGTCTC GGTCTC(1/5)
Bsp1407I 836 TGTACA T^GTACA
BspHI 503 TCATGA T^CATGA
BspMI 803 GCAGGT ACCTGC(4/8)
BsrFI 1270 GCCGGC [GA]^CCGG[CT]
BsrGI 836 TGTACA T^GTACA
BstUI 43 CGCG CG^CG
Cfr10I 1270 GCCGGC [GA]^CCGG[CT]
Eam1105I 778 GACTTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1205 AGCGCT AGC^GCT
FspI 545 TGCGCA TGC^GCA
HaeII 1205 AGCGCT [GA]GCGC^[CT]
HgaI 1097 GCGTC GACGC(5/10)
Hin1I 1096 GGCGTC G[GA]^CG[CT]C
HincII 1030 GTCAAC GT[CT]^[GA]AC
HindII 1030 GTCAAC GT[CT]^[GA]AC
HphI 74 TCACC GGTGA(8/7)
MaeI 139 CTAG C^TAG
MspA1I 823 CAGCTG C[AC]G^C[GT]G
MvnI 43 CGCG CG^CG
NaeI 1270 GCCGGC GCC^GGC
NgoMIV 1270 GCCGGC G^CCGGC
PflFI 1050 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 380 GGGTCCC [GA]G^G[AT]CC[CT]
PvuII 823 CAGCTG CAG^CTG
RcaI 503 TCATGA T^CATGA
SacI 142 GAGCTC GAGCT^C
SexAI 1148 ACCTGGT A^CC[AT]GGT
SfaNI 607 GATGC GCATC(5/9)
SmlI 10 CTCAAG C^T[CT][GA]AG
SspBI 836 TGTACA T^GTACA
StuI 666 AGGCCT AGG^CCT
Tsp509I 1236 AATT ^AATT
Tth111I 1050 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
BanI 364 GGTGCC G^G[CT][GA]CC
1186 GGTGCC
BcnI 147 CCGGG CC^[GC]GG
441 CCCGG
BpmI 652 CTGGAG CTGGAG(16/14)
670 CTGGAG
BseRI 1228 CTCCTC GAGGAG(10/8)
1243 GAGGAG
BsgI 521 GTGCAG GTGCAG(16/14)
929 GTGCAG
DraII 134 AGGCCCT [GA]G^G[ACGT]CC[CT]
380 GGGTCCC
EcoO109I 134 AGGCCCT [GA]G^G[ACGT]CC[CT]
380 GGGTCCC
EcoT14I 986 CCAAGG C^C[AT][AT]GG
1292 CCTTGG
FauI 44 GCGGG CCCGC(4/6)
632 GCGGG
MaeII 248 ACGT A^CGT
734 ACGT
MslI 612 CACCAATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
723 CATCGGCATG
NciI 147 CCGGG CC^[GC]GG
441 CCCGG
PflMI 939 CCAGGTAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1085 CCAATGGTTGG
StyI 986 CCAAGG C^C[AT][AT]GG
1292 CCTTGG
TaqI 284 TCGA T^CGA
875 TCGA
TfiI 48 GAATC G^A[AT]TC
935 GAATC
TthHB8I 284 TCGA T^CGA
875 TCGA
Van91I 939 CCAGGTAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1085 CCAATGGTTGG
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# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
BalI BbvI BmyI BsaJI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII EaeI EarI EcoRII Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII ItaI Ksp632I MaeIII
MboI MboII MflI MluNI MnlI
MscI MspI MvaI MwoI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI TseI Tsp45I TspRI
XhoII