Restriction map for
pF1KB5406
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# NO CUT ENZYMES ( 153 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI ApoI AscI Asp700I Asp718I
AspEI AspI AvaI AvaII AviII
AvrII BalI BamHI BanII BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BcnI BfrI BglI BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaWI BseAI BseRI BsiEI
BsiWI BsoBI BspEI BspLU11I BsrBI
BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstEII BstPI BstUI Bsu36I
CelII Cfr10I CpoI DraIII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FauI FbaI
FseI FspI HaeII HapII HgaI
Hin1I HincII HindII HpaI HpaII
KasI KpnI KspI MamI MfeI
MluI MluNI MroI MscI MslI
MspI MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI Psp1406I
PvuI RsrII SacI SacII SalI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI StuI
SwaI Tth111I Van91I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccI 84 GTATAC GT^[AC][GT]AC
AlwI 447 GATCC GGATC(4/5)
AlwNI 958 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 432 ATTAAT AT^TAAT
AsnI 432 ATTAAT AT^TAAT
AspHI 967 GTGCTC G[AT]GC[AT]^C
BanI 595 GGCACC G^G[CT][GA]CC
BglII 314 AGATCT A^GATCT
BmyI 967 GTGCTC G[AGT]GC[ACT]^C
BsgI 283 CTGCAC GTGCAG(16/14)
BsiHKAI 967 GTGCTC G[AT]GC[AT]^C
BsmBI 4 GAGACG CGTCTC(1/5)
BsmFI 159 GTCCC GGGAC(10/14)
BsmI 512 GCATTC GAATGC(1/-1)
Bsp1286I 967 GTGCTC G[AGT]GC[ACT]^C
BspDI 803 ATCGAT AT^CGAT
BspHI 95 TCATGA T^CATGA
Bst1107I 84 GTATAC GTA^TAC
BstXI 569 CCAAGTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 84 GTATAC GTA^TAC
CfoI 510 GCGC GCG^C
Cfr13I 960 GGCCC G^G[ACGT]CC
ClaI 803 ATCGAT AT^CGAT
DraI 376 TTTAAA TTT^AAA
DraII 959 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO109I 959 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 305 CCTTGG C^C[AT][AT]GG
HaeIII 960 GGCC GG^CC
HhaI 510 GCGC GCG^C
HinP1I 510 GCGC G^CGC
HindIII 176 AAGCTT A^AGCTT
MaeII 157 ACGT A^CGT
MspA1I 32 CAGCTG C[AC]G^C[GT]G
NlaIV 595 GGCACC GG[ACGT]^[ACGT]CC
NspI 251 GCATGC [GA]CATG^[CT]
PshBI 432 ATTAAT AT^TAAT
PvuII 32 CAGCTG CAG^CTG
RcaI 95 TCATGA T^CATGA
SapI 580 GAAGAGC GCTCTTC(1/4)
Sau96I 960 GGCCC G^G[ACGT]CC
SphI 251 GCATGC GCATG^C
SspI 607 AATATT AAT^ATT
StyI 305 CCTTGG C^C[AT][AT]GG
XcmI 605 CCAATATTGCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AciI 288 CCGC CCGC(-3/-1)
382 CCGC
BpmI 364 CTCCAG CTGGAG(16/14)
853 CTGGAG
BsaI 58 GAGACC GGTCTC(1/5)
856 GAGACC
BsaJI 305 CCTTGG C^C[ACGT][ACGT]GG
962 CCCTGG
Bsp1407I 491 TGTACA T^GTACA
660 TGTACA
BspMI 839 ACCTGC ACCTGC(4/8)
165 GCAGGT
BsrGI 491 TGTACA T^GTACA
660 TGTACA
BsrI 460 CCAGT ACTGG(1/-1)
685 CCAGT
BssKI 366 CCAGG ^CC[ACGT]GG
963 CCTGG
BstNI 366 CCAGG CC^[AT]GG
963 CCTGG
BstYI 314 AGATCT [GA]^GATC[CT]
446 AGATCC
DdeI 542 CTGAG C^T[ACGT]AG
675 CTCAG
EarI 426 CTCTTC CTCTTC(1/4)
580 GAAGAG
EcoRII 366 CCAGG ^CC[AT]GG
963 CCTGG
Ksp632I 426 CTCTTC CTCTTC(1/4)
580 GAAGAG
MaeIII 193 GTGAC ^GT[ACGT]AC
784 GTGAC
MflI 314 AGATCT [GA]^GATC[CT]
446 AGATCC
MseI 377 TTAA T^TAA
433 TTAA
MvaI 366 CCAGG CC^[AT]GG
963 CCTGG
MwoI 178 GCTTTTTTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
604 GCCAATATTGC
PstI 163 CTGCAG CTGCA^G
955 CTGCAG
SbfI 162 CCTGCAGG CCTGCA^GG
954 CCTGCAGG
ScrFI 366 CCAGG CC^[ACGT]GG
963 CCTGG
SmlI 53 CTCAAG C^T[CT][GA]AG
829 CTCAAG
Sse8387I 162 CCTGCAGG CCTGCA^GG
954 CCTGCAGG
SspBI 491 TGTACA T^GTACA
660 TGTACA
TaqI 669 TCGA T^CGA
804 TCGA
Tru9I 377 TTAA T^TAA
433 TTAA
Tsp45I 193 GTGAC ^GT[GC]AC
784 GTGAC
TthHB8I 669 TCGA T^CGA
804 TCGA
XhoII 314 AGATCT [GA]^GATC[CT]
446 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
AfaI AluI BbvI BfaI BsiYI
BslI BsmAI BstF5I Cac8I DpnII
Fnu4HI FokI HinfI HphI ItaI
MaeI MboI MboII MnlI NdeII
NlaIII PleI RsaI Sau3AI SfaNI
SfcI TfiI TseI Tsp509I TspRI