Restriction map for
pF1KB5384
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# NO CUT ENZYMES ( 172 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI BamHI BanI BanII BbeI
BbrPI BbvCI BciVI BclI BcnI
BfrI BglI BglII BlpI BmyI
Bpu1102I BsaAI BsaHI BsaI BsaWI
BseAI BsgI BsiEI BsiHKAI BsiWI
BsmAI BsmBI BsmI Bsp1286I Bsp1407I
BspEI BspHI BspLU11I BsrFI BsrGI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstYI BstZ17I
Bsu36I CelII Cfr10I CpoI DdeI
DraI DraII DraIII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI HaeII HapII
Hin1I HincII HindII HpaI HpaII
KasI KpnI KspI MfeI MflI
MluI MroI MslI MspA1I MspI
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tth111I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AlwI 539 GGATC GGATC(4/5)
AvaI 422 CCCGAG C^[CT]CG[GA]G
AviII 20 TGCGCA TGC^GCA
AvrII 240 CCTAGG C^CTAGG
BbsI 7 GAAGAC GAAGAC(2/6)
BcgI 503 GCAATGAGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 240 CCTAGG C^CTAGG
BpmI 31 CTGGAG CTGGAG(16/14)
BpuAI 7 GAAGAC GAAGAC(2/6)
BsaBI 378 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 34 GAGGAG GAGGAG(10/8)
BsmFI 269 GTCCC GGGAC(10/14)
BsoBI 422 CCCGAG C^[CT]CG[GA]G
BspDI 511 ATCGAT AT^CGAT
BspMI 453 GCAGGT ACCTGC(4/8)
BsrBI 459 GAGCGG CCGCTC(-3/-3)
BssSI 353 CTCGTG CACGAG(-5/-1)
CfoI 21 GCGC GCG^C
ClaI 511 ATCGAT AT^CGAT
EarI 109 GAAGAG CTCTTC(1/4)
EcoNI 447 CCTAGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 240 CCTAGG C^C[AT][AT]GG
FauI 254 GCGGG CCCGC(4/6)
FspI 20 TGCGCA TGC^GCA
HgaI 10 GACGC GACGC(5/10)
HhaI 21 GCGC GCG^C
HinP1I 21 GCGC G^CGC
HindIII 280 AAGCTT A^AGCTT
HphI 456 GGTGA GGTGA(8/7)
Ksp632I 109 GAAGAG CTCTTC(1/4)
MaeII 591 ACGT A^CGT
MamI 378 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 284 TTAA T^TAA
NlaIV 473 GGAACC GG[ACGT]^[ACGT]CC
NspI 17 ACATGC [GA]CATG^[CT]
PleI 70 GAGTC GAGTC(4/5)
StyI 240 CCTAGG C^C[AT][AT]GG
Tru9I 284 TTAA T^TAA
Tsp45I 482 GTCAC ^GT[GC]AC
TspRI 387 CAGTG CA[GC]TG[ACGT][ACGT]^
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# TWO CUT ENZYMES ( 21 enzymes) #
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AcsI 228 GAATTT [GA]^AATT[CT]
247 AAATTC
AluI 29 AGCT AG^CT
281 AGCT
ApoI 228 GAATTT [GA]^AATT[CT]
247 AAATTC
AvaII 192 GGACC G^G[AT]CC
237 GGACC
BalI 338 TGGCCA TGG^CCA
380 TGGCCA
BbvI 42 GCAGC GCAGC(8/12)
96 GCTGC
BfaI 241 CTAG C^TAG
448 CTAG
BsrDI 23 GCAATG GCAATG(2/0)
503 GCAATG
BssKI 311 CCTGG ^CC[ACGT]GG
492 CCTGG
BstNI 311 CCTGG CC^[AT]GG
492 CCTGG
EcoRII 311 CCTGG ^CC[AT]GG
492 CCTGG
MaeI 241 CTAG C^TAG
448 CTAG
MboII 7 GAAGA GAAGA(8/7)
109 GAAGA
MluNI 338 TGGCCA TGG^CCA
380 TGGCCA
MscI 338 TGGCCA TGG^CCA
380 TGGCCA
MvaI 311 CCTGG CC^[AT]GG
492 CCTGG
ScrFI 311 CCTGG CC^[ACGT]GG
492 CCTGG
TaqI 512 TCGA T^CGA
533 TCGA
TfiI 522 GAATC G^A[AT]TC
556 GATTC
TseI 42 GCAGC G^C[AT]GC
96 GCTGC
TthHB8I 512 TCGA T^CGA
533 TCGA
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# 3 OR MORE CUT ENZYMES ( 25 enzymes) #
#########################################
AciI BsaJI BsiYI BslI BsrI
BstF5I Cac8I Cfr13I DpnII EaeI
Fnu4HI FokI HaeIII HinfI ItaI
MaeIII MboI MnlI MwoI NdeII
NlaIII Sau3AI Sau96I SfaNI Tsp509I