Restriction map for
pF1KB5377
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# NO CUT ENZYMES ( 125 enzymes) #
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AatII AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI AspEI AspI
AviII AvrII BalI BamHI BbrPI
BcgI BciVI BclI BfaI BfrI
BlnI BsaAI BsaWI BseAI BsiWI
BsmBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstEII BstF5I
BstPI BstXI BstZ17I Bsu36I Cfr10I
ClaI CpoI DraIII EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoT22I FbaI FokI FseI
FspI HincII HindII HindIII HpaI
MaeI MfeI MluI MluNI MroI
MscI MunI NaeI NdeI NgoMIV
NheI NotI NruI NsiI PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacI SalI
SapI SbfI ScaI SexAI SfaNI
SfiI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI SwaI Tsp509I Tth111I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 57 enzymes) #
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Acc65I 259 GGTACC G^GTACC
AcyI 118 GGCGCC G[GA]^CG[CT]C
Asp700I 970 GAAAGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 259 GGTACC G^GTACC
AspHI 160 GTGCTC G[AT]GC[AT]^C
AvaI 922 CCCGAG C^[CT]CG[GA]G
AvaII 936 GGACC G^G[AT]CC
BbeI 118 GGCGCC GGCGC^C
BbsI 598 GAAGAC GAAGAC(2/6)
BbvCI 145 GCTGAGG CCTCAGC(-5/-2)
BglI 227 GCCCCAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 162 GCTCAGC GC^T[ACGT]AGC
BpmI 893 CTGGAG CTGGAG(16/14)
Bpu1102I 162 GCTCAGC GC^T[ACGT]AGC
BpuAI 598 GAAGAC GAAGAC(2/6)
BsaBI 546 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 118 GGCGCC G[GA]^CG[CT]C
BsgI 804 CTGCAC GTGCAG(16/14)
BsiEI 192 CGACCG CG[GA][CT]^CG
BsiHKAI 160 GTGCTC G[AT]GC[AT]^C
BsmI 587 GCATTC GAATGC(1/-1)
BsoBI 922 CCCGAG C^[CT]CG[GA]G
BspMI 1005 GCAGGT ACCTGC(4/8)
BstBI 590 TTCGAA TT^CGAA
CelII 162 GCTCAGC GC^T[ACGT]AGC
DraI 714 TTTAAA TTT^AAA
DraII 935 GGGACCT [GA]G^G[ACGT]CC[CT]
DrdI 499 GACCTTCGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 438 TGGCCG [CT]^GGCC[GA]
EarI 517 GAAGAG CTCTTC(1/4)
EcoO109I 935 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoRV 700 GATATC GAT^ATC
HgaI 43 GCGTC GACGC(5/10)
Hin1I 118 GGCGCC G[GA]^CG[CT]C
KasI 118 GGCGCC G^GCGCC
KpnI 259 GGTACC GGTAC^C
Ksp632I 517 GAAGAG CTCTTC(1/4)
KspI 36 CCGCGG CCGC^GG
MaeIII 490 GTCAC ^GT[ACGT]AC
MamI 546 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 118 GGCGCC GG^CGCC
NcoI 282 CCATGG C^CATGG
NspI 875 GCATGC [GA]CATG^[CT]
NspV 590 TTCGAA TT^CGAA
PleI 506 GAGTC GAGTC(4/5)
PpuMI 935 GGGACCT [GA]G^G[AT]CC[CT]
PvuII 695 CAGCTG CAG^CTG
SacII 36 CCGCGG CCGC^GG
SfcI 525 CTACAG C^T[GA][CT]AG
SfoI 118 GGCGCC GGC^GCC
SfuI 590 TTCGAA TT^CGAA
SmlI 1000 CTTGAG C^T[CT][GA]AG
SphI 875 GCATGC GCATG^C
SspI 606 AATATT AAT^ATT
TfiI 975 GATTC G^A[AT]TC
Tsp45I 490 GTCAC ^GT[GC]AC
XmnI 970 GAAAGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
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AluI 271 AGCT AG^CT
696 AGCT
AlwI 481 GATCC GGATC(4/5)
776 GGATC
AlwNI 79 CAGGCGCTG CAG[ACGT][ACGT][ACGT]^CTG
528 CAGCGGCTG
BanII 112 GAGCCC G[GA]GC[CT]^C
460 GAGCCC
BglII 185 AGATCT A^GATCT
521 AGATCT
BsaI 287 GAGACC GGTCTC(1/5)
671 GAGACC
BseRI 52 GAGGAG GAGGAG(10/8)
124 GAGGAG
BsmFI 935 GGGAC GGGAC(10/14)
817 GTCCC
BsrBI 441 CCGCTC CCGCTC(-3/-3)
447 CCGCTC
BsrI 230 CCAGT ACTGG(1/-1)
487 CCAGT
DsaI 36 CCGCGG C^C[GA][CT]GG
282 CCATGG
EcoNI 783 CCTCCGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
892 CCTGGAGAAGG
EcoT14I 282 CCATGG C^C[AT][AT]GG
662 CCAAGG
HaeII 81 GGCGCT [GA]GCGC^[CT]
118 GGCGCC
HinfI 506 GAGTC G^A[ACGT]TC
975 GATTC
HphI 422 TCACC GGTGA(8/7)
491 TCACC
MseI 715 TTAA T^TAA
740 TTAA
MslI 423 CACCAACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
615 CATCGAAGTG
PflMI 18 CCAGACGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
388 CCATTCTCTGG
StyI 282 CCATGG C^C[AT][AT]GG
662 CCAAGG
Tru9I 715 TTAA T^TAA
740 TTAA
TspRI 231 CAGTG CA[GC]TG[ACGT][ACGT]^
809 CAGTG
Van91I 18 CCAGACGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
388 CCATTCTCTGG
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# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AccII AciI AfaI BanI BbvI
BcnI BmyI BsaJI BsiYI BslI
BsmAI Bsp1286I BssKI BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII EcoRII FauI Fnu4HI HaeIII
HapII HhaI HinP1I HpaII ItaI
MaeII MboI MboII MflI MnlI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI StuI TaqI
TseI TthHB8I XhoII