Restriction map for
pF1KB5344
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# NO CUT ENZYMES ( 156 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwNI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaI AvaII
AviII AvrII BamHI BbrPI BbvCI
BclI BcnI BfrI BglI BglII
BlnI BpmI BsaAI BsaJI BsaWI
BseAI BseRI BsiEI BsiHKAI BsiWI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstEII BstPI BstXI BstZ17I Cfr10I
ClaI CpoI DraI DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI FseI FspI
HapII HgaI HincII HindII HindIII
HpaI HpaII KpnI KspI MaeIII
MfeI MluI MroI MseI MslI
MspI MunI NaeI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SalI SbfI SexAI SfiI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
TfiI Tru9I Tsp45I Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 68 enzymes) #
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AcyI 432 GGCGCC G[GA]^CG[CT]C
AfaI 401 GTAC GT^AC
AlwI 208 GGATC GGATC(4/5)
AocI 203 CCTCAGG CC^T[ACGT]AGG
ApaI 14 GGGCCC GGGCC^C
BalI 32 TGGCCA TGG^CCA
BanI 432 GGCGCC G^G[CT][GA]CC
BbeI 432 GGCGCC GGCGC^C
BbsI 75 GAAGAC GAAGAC(2/6)
BcgI 249 CGACTCGGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 198 GGATAC GTATCC(6/5)
BlpI 500 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 500 GCTCAGC GC^T[ACGT]AGC
BpuAI 75 GAAGAC GAAGAC(2/6)
BsaBI 106 GATTGCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 432 GGCGCC G[GA]^CG[CT]C
BsaI 118 GAGACC GGTCTC(1/5)
BsiYI 423 CCAGAAGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 423 CCAGAAGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmBI 9 GAGACG CGTCTC(1/5)
BspMI 383 ACCTGC ACCTGC(4/8)
BssKI 492 CCTGG ^CC[ACGT]GG
BstBI 139 TTCGAA TT^CGAA
BstNI 492 CCTGG CC^[AT]GG
BstYI 208 GGATCT [GA]^GATC[CT]
Bsu36I 203 CCTCAGG CC^T[ACGT]AGG
Cac8I 429 GCAGGC GC[ACGT]^[ACGT]GC
CelII 500 GCTCAGC GC^T[ACGT]AGC
DpnII 209 GATC ^GATC
DraII 14 GGGCCCC [GA]G^G[ACGT]CC[CT]
EaeI 32 TGGCCA [CT]^GGCC[GA]
Eco81I 203 CCTCAGG CC^T[ACGT]AGG
EcoO109I 14 GGGCCCC [GA]G^G[ACGT]CC[CT]
EcoRII 492 CCTGG ^CC[AT]GG
FauI 353 CCCGC CCCGC(4/6)
HaeII 432 GGCGCC [GA]GCGC^[CT]
Hin1I 432 GGCGCC G[GA]^CG[CT]C
HinfI 250 GACTC G^A[ACGT]TC
HphI 509 TCACC GGTGA(8/7)
KasI 432 GGCGCC G^GCGCC
MaeII 62 ACGT A^CGT
MamI 106 GATTGCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 209 GATC ^GATC
MflI 208 GGATCT [GA]^GATC[CT]
MluNI 32 TGGCCA TGG^CCA
MscI 32 TGGCCA TGG^CCA
MspA1I 472 CAGCTG C[AC]G^C[GT]G
MvaI 492 CCTGG CC^[AT]GG
MwoI 524 GCATCCACAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NarI 432 GGCGCC GG^CGCC
NdeII 209 GATC ^GATC
NlaIII 552 CATG CATG^
NspV 139 TTCGAA TT^CGAA
PleI 250 GACTC GAGTC(4/5)
PvuII 472 CAGCTG CAG^CTG
RsaI 401 GTAC GT^AC
SapI 588 GAAGAGC GCTCTTC(1/4)
Sau3AI 209 GATC ^GATC
ScaI 400 AGTACT AGT^ACT
ScrFI 492 CCTGG CC^[ACGT]GG
SfcI 243 CTACAG C^T[GA][CT]AG
SfoI 432 GGCGCC GGC^GCC
SfuI 139 TTCGAA TT^CGAA
SmlI 310 CTCAAG C^T[CT][GA]AG
TaqI 140 TCGA T^CGA
Tsp509I 145 AATT ^AATT
TthHB8I 140 TCGA T^CGA
XhoII 208 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 20 enzymes) #
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AccII 81 CGCG CG^CG
355 CGCG
AciI 80 CCGC CCGC(-3/-1)
354 CCGC
BanII 14 GGGCCC G[GA]GC[CT]^C
591 GAGCCC
BbvI 471 GCAGC GCAGC(8/12)
371 GCTGC
BfaI 334 CTAG C^TAG
572 CTAG
BsgI 64 GTGCAG GTGCAG(16/14)
479 GTGCAG
BsrI 25 CCAGT ACTGG(1/-1)
632 CCAGT
BstUI 81 CGCG CG^CG
355 CGCG
CfoI 305 GCGC GCG^C
433 GCGC
Cfr13I 14 GGGCC G^G[ACGT]CC
15 GGCCC
EarI 651 CTCTTC CTCTTC(1/4)
588 GAAGAG
Fnu4HI 371 GCTGC GC^[ACGT]GC
471 GCAGC
HhaI 305 GCGC GCG^C
433 GCGC
HinP1I 305 GCGC G^CGC
433 GCGC
ItaI 371 GCTGC GC^[ACGT]GC
471 GCAGC
Ksp632I 651 CTCTTC CTCTTC(1/4)
588 GAAGAG
MaeI 334 CTAG C^TAG
572 CTAG
MvnI 81 CGCG CG^CG
355 CGCG
Sau96I 14 GGGCC G^G[ACGT]CC
15 GGCCC
TseI 371 GCTGC G^C[AT]GC
471 GCAGC
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# 3 OR MORE CUT ENZYMES ( 14 enzymes) #
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AluI BmyI BsmAI BsmFI Bsp1286I
BstF5I DdeI FokI HaeIII MboII
MnlI NlaIV SfaNI TspRI