Restriction map for
pF1KB5332
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# NO CUT ENZYMES ( 139 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI Asp700I
Asp718I AspEI AspI AvaII AviII
AvrII BamHI BanI BbeI BbrPI
BciVI BclI BfaI BfrI BglI
BglII BlnI BlpI BpmI Bpu1102I
BsaBI BsaHI BseAI BsiEI BsmI
Bsp1407I BspDI BspEI BspMI BsrBI
BsrFI BsrGI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
CelII CfoI Cfr10I ClaI CpoI
DraI DraII DsaI EagI Eam1105I
EarI EclXI Eco47III Eco52I EcoO109I
EcoO65I EcoRV EcoT14I EcoT22I FauI
FbaI FseI FspI HaeII HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI KasI KpnI Ksp632I KspI
MaeI MamI MfeI MluI MroI
MspA1I MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PvuI PvuII RsrII SacII
SalI SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
Sse8387I SspBI SspI StuI StyI
SwaI Tth111I XbaI XmnI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AccII 335 CGCG CG^CG
AcsI 184 GAATTC [GA]^AATT[CT]
AlwNI 469 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 573 CCTGAGG CC^T[ACGT]AGG
ApoI 184 GAATTC [GA]^AATT[CT]
AseI 207 ATTAAT AT^TAAT
AsnI 207 ATTAAT AT^TAAT
BanII 37 GAGCTC G[GA]GC[CT]^C
BbvCI 118 GCTGAGG CCTCAGC(-5/-2)
BbvI 243 GCTGC GCAGC(8/12)
BcgI 246 GCAGTACTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaAI 424 TACGTG [CT]AC^GT[GA]
BsaI 353 GAGACC GGTCTC(1/5)
BsaWI 384 TCCGGT [AT]^CCGG[AT]
BseRI 271 GAGGAG GAGGAG(10/8)
BsgI 261 CTGCAC GTGCAG(16/14)
BsiWI 5 CGTACG C^GTACG
BsmBI 347 CGTCTC CGTCTC(1/5)
BsmFI 257 GGGAC GGGAC(10/14)
BspHI 177 TCATGA T^CATGA
BsrI 143 ACTGG ACTGG(1/-1)
BssSI 793 CACGAG CACGAG(-5/-1)
BstUI 335 CGCG CG^CG
BstYI 797 AGATCC [GA]^GATC[CT]
Bsu36I 573 CCTGAGG CC^T[ACGT]AGG
Cfr13I 465 GGGCC G^G[ACGT]CC
DraIII 580 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 46 GACGAGAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 573 CCTGAGG CC^T[ACGT]AGG
EcoNI 532 CCTACTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 184 GAATTC G^AATTC
Fnu4HI 243 GCTGC GC^[ACGT]GC
ItaI 243 GCTGC GC^[ACGT]GC
MflI 797 AGATCC [GA]^GATC[CT]
MslI 381 CATTCCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 335 CGCG CG^CG
PaeR7I 350 CTCGAG C^TCGAG
PflMI 137 CCATTGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 207 ATTAAT AT^TAAT
RcaI 177 TCATGA T^CATGA
SacI 37 GAGCTC GAGCT^C
Sau96I 465 GGGCC G^G[ACGT]CC
SfaNI 157 GATGC GCATC(5/9)
SmaI 374 CCCGGG CCC^GGG
SmlI 350 CTCGAG C^T[CT][GA]AG
SphI 516 GCATGC GCATG^C
TfiI 99 GATTC G^A[AT]TC
TseI 243 GCTGC G^C[AT]GC
Van91I 137 CCATTGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 416 CCAATGACTACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 350 CTCGAG C^TCGAG
XhoII 797 AGATCC [GA]^GATC[CT]
XmaCI 374 CCCGGG C^CCGGG
XmaI 374 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AflIII 265 ACATGT A^C[GA][CT]GT
339 ACATGT
AspHI 37 GAGCTC G[AT]GC[AT]^C
288 GTGCTC
BalI 89 TGGCCA TGG^CCA
607 TGGCCA
BbsI 325 GAAGAC GAAGAC(2/6)
109 GTCTTC
BmyI 37 GAGCTC G[AGT]GC[ACT]^C
288 GTGCTC
BpuAI 325 GAAGAC GAAGAC(2/6)
109 GTCTTC
BsiHKAI 37 GAGCTC G[AT]GC[AT]^C
288 GTGCTC
Bsp1286I 37 GAGCTC G[AGT]GC[ACT]^C
288 GTGCTC
BspLU11I 265 ACATGT A^CATGT
339 ACATGT
BsrDI 189 CATTGC GCAATG(2/0)
320 GCAATG
BstNI 81 CCTGG CC^[AT]GG
468 CCAGG
EaeI 89 TGGCCA [CT]^GGCC[GA]
607 TGGCCA
EcoRII 81 CCTGG ^CC[AT]GG
468 CCAGG
HgaI 333 GACGC GACGC(5/10)
717 GCGTC
HphI 387 GGTGA GGTGA(8/7)
302 TCACC
MaeII 346 ACGT A^CGT
425 ACGT
MaeIII 343 GTCAC ^GT[ACGT]AC
388 GTGAC
MluNI 89 TGGCCA TGG^CCA
607 TGGCCA
MscI 89 TGGCCA TGG^CCA
607 TGGCCA
MseI 208 TTAA T^TAA
787 TTAA
MvaI 81 CCTGG CC^[AT]GG
468 CCAGG
PleI 643 GAGTC GAGTC(4/5)
700 GAGTC
PstI 244 CTGCAG CTGCA^G
536 CTGCAG
ScaI 248 AGTACT AGT^ACT
620 AGTACT
SfcI 244 CTGCAG C^T[GA][CT]AG
536 CTGCAG
Tru9I 208 TTAA T^TAA
787 TTAA
Tsp45I 343 GTCAC ^GT[GC]AC
388 GTGAC
TspRI 42 CACTG CA[GC]TG[ACGT][ACGT]^
584 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaI
BcnI BsaJI BsiYI BslI BsmAI
BsoBI BssKI BstF5I Cac8I DdeI
DpnII FokI HaeIII HapII HinfI
HpaII MboI MboII MnlI MspI
MwoI NciI NdeII NlaIII NlaIV
NspI RsaI Sau3AI ScrFI TaqI
Tsp509I TthHB8I