Restriction map for
pF1KB5291
##################################
# NO CUT ENZYMES ( 175 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AfaI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
AscI AseI AsnI Asp718I AspEI
AspHI AspI AviII AvrII BalI
BamHI BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BcnI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsiEI BsiHKAI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrFI BsrGI
BsrI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraI DraIII DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
HaeII HapII HgaI HhaI Hin1I
HinP1I HindIII HinfI HpaI HpaII
KasI KpnI KspI MaeIII MamI
MfeI MluI MluNI MroI MscI
MseI MspA1I MspI MunI MvnI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsaI RsrII SacI SacII
SbfI ScaI SfcI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TfiI Tru9I Tsp45I
Tth111I XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AccI 277 GTCGAC GT^[AC][GT]AC
AciI 350 CCGC CCGC(-3/-1)
AcsI 29 GAATTT [GA]^AATT[CT]
AlwNI 9 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 202 GGGCCC GGGCC^C
ApoI 29 GAATTT [GA]^AATT[CT]
Asp700I 425 GAATAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 175 CTCGGG C^[CT]CG[GA]G
AvaII 242 GGACC G^G[AT]CC
BanII 202 GGGCCC G[GA]GC[CT]^C
BfaI 229 CTAG C^TAG
BpmI 283 CTGGAG CTGGAG(16/14)
BseRI 187 GAGGAG GAGGAG(10/8)
BsgI 485 CTGCAC GTGCAG(16/14)
BsiYI 8 CCAGAACCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 8 CCAGAACCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 241 GGGAC GGGAC(10/14)
BsoBI 175 CTCGGG C^[CT]CG[GA]G
BspMI 262 GCAGGT ACCTGC(4/8)
BsrDI 78 CATTGC GCAATG(2/0)
BstYI 145 AGATCC [GA]^GATC[CT]
DraII 201 GGGGCCC [GA]G^G[ACGT]CC[CT]
DsaI 206 CCATGG C^C[GA][CT]GG
EarI 66 CTCTTC CTCTTC(1/4)
EcoO109I 201 GGGGCCC [GA]G^G[ACGT]CC[CT]
EcoT14I 206 CCATGG C^C[AT][AT]GG
HincII 277 GTCGAC GT[CT]^[GA]AC
HindII 277 GTCGAC GT[CT]^[GA]AC
HphI 74 TCACC GGTGA(8/7)
Ksp632I 66 CTCTTC CTCTTC(1/4)
MaeI 229 CTAG C^TAG
MaeII 308 ACGT A^CGT
MboII 67 TCTTC GAAGA(8/7)
MflI 145 AGATCC [GA]^GATC[CT]
MwoI 38 GCCTCTTTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 206 CCATGG C^CATGG
NspI 399 ACATGC [GA]CATG^[CT]
PflMI 8 CCAGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SalI 277 GTCGAC G^TCGAC
SapI 65 GCTCTTC GCTCTTC(1/4)
SexAI 13 ACCTGGT A^CC[AT]GGT
SfaNI 495 GCATC GCATC(5/9)
StyI 206 CCATGG C^C[AT][AT]GG
TaqI 278 TCGA T^CGA
TspRI 488 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 278 TCGA T^CGA
Van91I 8 CCAGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 228 TCTAGA T^CTAGA
XhoII 145 AGATCC [GA]^GATC[CT]
XmnI 425 GAATAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AluI 222 AGCT AG^CT
483 AGCT
BanI 3 GGCACC G^G[CT][GA]CC
17 GGTGCC
BbvI 220 GCAGC GCAGC(8/12)
484 GCTGC
BmyI 18 GTGCCC G[AGT]GC[ACT]^C
202 GGGCCC
Bsp1286I 18 GTGCCC G[AGT]GC[ACT]^C
202 GGGCCC
BstF5I 105 CATCC GGATG(2/0)
134 GGATG
BstXI 206 CCATGGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
245 CCAGGGCTATGG
Cac8I 61 GCCTGC GC[ACGT]^[ACGT]GC
162 GCAGGC
DdeI 124 CTTAG C^T[ACGT]AG
356 CTCAG
Fnu4HI 220 GCAGC GC^[ACGT]GC
484 GCTGC
FokI 105 CATCC GGATG(9/13)
134 GGATG
HaeIII 37 GGCC GG^CC
203 GGCC
ItaI 220 GCAGC GC^[ACGT]GC
484 GCTGC
MslI 105 CATCCTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
207 CATGGGCGTG
TseI 220 GCAGC G^C[AT]GC
484 GCTGC
#########################################
# 3 OR MORE CUT ENZYMES ( 18 enzymes) #
#########################################
AlwI BsaJI BsmAI BssKI BstNI
Cfr13I DpnII EcoRII MboI MnlI
MvaI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI Tsp509I