Restriction map for
pF1KB5282
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# NO CUT ENZYMES ( 156 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflIII AgeI AhdI AluI
Alw44I AlwI AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AvaI AviII
AvrII BamHI BanII BbrPI BbsI
BbvCI BcgI BciVI BclI BfaI
BglI BlnI BlpI BpmI Bpu1102I
BpuAI BsaI BsaWI BseAI BsgI
BsiEI BsiWI BsmBI BsmFI BsmI
BsoBI Bsp1407I BspEI BspHI BspLU11I
BspMI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I Bsu36I CelII
Cfr10I CpoI DraI DraII DraIII
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI HincII HindII HindIII HpaI
KpnI KspI MaeI MfeI MluI
MroI MspA1I MunI MvnI MwoI
NaeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TspRI
Tth111I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AciI 162 GCGG CCGC(-3/-1)
AfaI 90 GTAC GT^AC
AflII 265 CTTAAG C^TTAAG
Asp700I 78 GAATTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 649 GAGCAC G[AT]GC[AT]^C
BanI 21 GGCGCC G^G[CT][GA]CC
BbeI 21 GGCGCC GGCGC^C
BbvI 11 GCAGC GCAGC(8/12)
BcnI 5 CCGGG CC^[GC]GG
BfrI 265 CTTAAG C^TTAAG
BglII 621 AGATCT A^GATCT
BmyI 649 GAGCAC G[AGT]GC[ACT]^C
BsaAI 87 CACGTA [CT]AC^GT[GA]
BsaBI 622 GATCTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 16 GAGGAG GAGGAG(10/8)
BsiHKAI 649 GAGCAC G[AT]GC[AT]^C
Bsp1286I 649 GAGCAC G[AGT]GC[ACT]^C
BspDI 290 ATCGAT AT^CGAT
BsrBI 160 GAGCGG CCGCTC(-3/-3)
BstNI 64 CCAGG CC^[AT]GG
BstXI 503 CCAGTTTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 621 AGATCT [GA]^GATC[CT]
CfoI 22 GCGC GCG^C
ClaI 290 ATCGAT AT^CGAT
DrdI 202 GACATCATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 350 CCATGG C^C[GA][CT]GG
EarI 516 GAAGAG CTCTTC(1/4)
EcoRII 64 CCAGG ^CC[AT]GG
HaeII 21 GGCGCC [GA]GCGC^[CT]
HapII 5 CCGG C^CGG
HhaI 22 GCGC GCG^C
HinP1I 22 GCGC G^CGC
HpaII 5 CCGG C^CGG
KasI 21 GGCGCC G^GCGCC
Ksp632I 516 GAAGAG CTCTTC(1/4)
MaeII 88 ACGT A^CGT
MamI 622 GATCTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboII 516 GAAGA GAAGA(8/7)
MflI 621 AGATCT [GA]^GATC[CT]
MslI 100 CACTATTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 5 CCGG C^CGG
MvaI 64 CCAGG CC^[AT]GG
NarI 21 GGCGCC GG^CGCC
NciI 5 CCGGG CC^[GC]GG
NcoI 350 CCATGG C^CATGG
NdeI 633 CATATG CA^TATG
NlaIV 21 GGCGCC GG[ACGT]^[ACGT]CC
PflMI 345 CCAGTCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 671 GAGTC GAGTC(4/5)
RsaI 90 GTAC GT^AC
SfaNI 360 GATGC GCATC(5/9)
SfoI 21 GGCGCC GGC^GCC
SmlI 265 CTTAAG C^T[CT][GA]AG
TseI 11 GCAGC G^C[AT]GC
Van91I 345 CCAGTCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 621 AGATCT [GA]^GATC[CT]
XmnI 78 GAATTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcyI 21 GGCGCC G[GA]^CG[CT]C
40 GGCGTC
AvaII 210 GGTCC G^G[AT]CC
443 GGACC
BalI 61 TGGCCA TGG^CCA
167 TGGCCA
BsaHI 21 GGCGCC G[GA]^CG[CT]C
40 GGCGTC
BsiYI 25 CCAGACTACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
345 CCAGTCCATGG
BslI 25 CCAGACTACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
345 CCAGTCCATGG
BsmAI 110 GAGAC GTCTC(1/5)
298 GTCTC
BssKI 5 CCGGG ^CC[ACGT]GG
64 CCAGG
Cac8I 165 GCTGGC GC[ACGT]^[ACGT]GC
393 GCTGGC
Cfr13I 210 GGTCC G^G[ACGT]CC
443 GGACC
DdeI 460 CTCAG C^T[ACGT]AG
597 CTTAG
EcoT14I 170 CCAAGG C^C[AT][AT]GG
350 CCATGG
Fnu4HI 11 GCAGC GC^[ACGT]GC
162 GCGGC
HgaI 41 GCGTC GACGC(5/10)
638 GCGTC
Hin1I 21 GGCGCC G[GA]^CG[CT]C
40 GGCGTC
HphI 355 GGTGA GGTGA(8/7)
513 GGTGA
ItaI 11 GCAGC GC^[ACGT]GC
162 GCGGC
MluNI 61 TGGCCA TGG^CCA
167 TGGCCA
MscI 61 TGGCCA TGG^CCA
167 TGGCCA
Sau96I 210 GGTCC G^G[ACGT]CC
443 GGACC
ScrFI 5 CCGGG CC^[ACGT]GG
64 CCAGG
StyI 170 CCAAGG C^C[AT][AT]GG
350 CCATGG
TaqI 291 TCGA T^CGA
669 TCGA
TfiI 567 GATTC G^A[AT]TC
627 GAATC
Tsp45I 200 GTGAC ^GT[GC]AC
533 GTGAC
TthHB8I 291 TCGA T^CGA
669 TCGA
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# 3 OR MORE CUT ENZYMES ( 19 enzymes) #
#########################################
AcsI ApoI BsaJI BsrI BstF5I
DpnII EaeI FokI HaeIII HinfI
MaeIII MboI MnlI MseI NdeII
NlaIII Sau3AI Tru9I Tsp509I