Restriction map for
pF1KB5275
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# NO CUT ENZYMES ( 137 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AfaI AflII AgeI
AhdI Alw44I Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvrII BamHI BbeI
BbrPI BbsI BcgI BciVI BclI
BfrI BglII BlnI BpuAI BsaAI
BsaBI BsaHI BsaWI BseAI BsiEI
BsiWI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrGI
BssHII BssSI Bst1107I BstBI BstUI
BstXI BstZ17I ClaI CpoI DraI
DraIII EagI Eam1105I EarI EclXI
Eco47III Eco52I EcoRI EcoRV EcoT14I
EcoT22I FbaI FseI HgaI Hin1I
HincII HindII HindIII HpaI KasI
KpnI Ksp632I KspI MamI MfeI
MluI MroI MunI MvnI NarI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StyI
SwaI TaqI Tth111I TthHB8I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AccI 1201 GTCTAC GT^[AC][GT]AC
AflIII 446 ACGTGT A^C[GA][CT]GT
AspHI 340 GAGCAC G[AT]GC[AT]^C
AviII 1325 TGCGCA TGC^GCA
BalI 953 TGGCCA TGG^CCA
BbvCI 741 CCTCAGC CCTCAGC(-5/-2)
BlpI 855 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 855 GCTGAGC GC^T[ACGT]AGC
BsaI 183 GAGACC GGTCTC(1/5)
BsgI 696 GTGCAG GTGCAG(16/14)
BsiHKAI 340 GAGCAC G[AT]GC[AT]^C
BsrFI 140 GCCGGC [GA]^CCGG[CT]
BstAPI 342 GCACAACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 981 GGTCACC G^GT[ACGT]ACC
BstPI 981 GGTCACC G^GT[ACGT]ACC
CelII 855 GCTGAGC GC^T[ACGT]AGC
Cfr10I 140 GCCGGC [GA]^CCGG[CT]
DrdI 391 GACGAGCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 872 CCGTGG C^C[GA][CT]GG
EaeI 953 TGGCCA [CT]^GGCC[GA]
EcoNI 321 CCTGCGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 981 GGTCACC G^GT[ACGT]ACC
FspI 1325 TGCGCA TGC^GCA
MluNI 953 TGGCCA TGG^CCA
MscI 953 TGGCCA TGG^CCA
MseI 827 TTAA T^TAA
NaeI 140 GCCGGC GCC^GGC
NgoMIV 140 GCCGGC G^CCGGC
PflMI 645 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1013 GAGTC GAGTC(4/5)
PpuMI 1248 AGGTCCT [GA]G^G[AT]CC[CT]
StuI 1389 AGGCCT AGG^CCT
TfiI 766 GATTC G^A[AT]TC
Tru9I 827 TTAA T^TAA
Tsp509I 907 AATT ^AATT
Van91I 645 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 968 CCACGCTCCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AlwNI 646 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
1130 CAGCCCCTG
AocI 259 CCTGAGG CC^T[ACGT]AGG
1252 CCTGAGG
BfaI 121 CTAG C^TAG
1144 CTAG
BsmBI 711 CGTCTC CGTCTC(1/5)
497 GAGACG
BspMI 347 ACCTGC ACCTGC(4/8)
1322 ACCTGC
BstYI 842 AGATCC [GA]^GATC[CT]
1088 AGATCC
Bsu36I 259 CCTGAGG CC^T[ACGT]AGG
1252 CCTGAGG
Eco81I 259 CCTGAGG CC^T[ACGT]AGG
1252 CCTGAGG
HaeII 700 AGCGCC [GA]GCGC^[CT]
859 AGCGCC
HinfI 766 GATTC G^A[ACGT]TC
1013 GAGTC
MaeI 121 CTAG C^TAG
1144 CTAG
MflI 842 AGATCC [GA]^GATC[CT]
1088 AGATCC
NspI 941 GCATGT [GA]CATG^[CT]
1197 GCATGT
SfaNI 831 GATGC GCATC(5/9)
882 GATGC
SmaI 74 CCCGGG CCC^GGG
716 CCCGGG
XhoII 842 AGATCC [GA]^GATC[CT]
1088 AGATCC
XmaCI 74 CCCGGG C^CCGGG
716 CCCGGG
XmaI 74 CCCGGG C^CCGGG
716 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AciI AluI AlwI ApaI AvaI
AvaII BanI BanII BbvI BcnI
BglI BmyI BpmI BsaJI BseRI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HpaII HphI ItaI MaeII MaeIII
MboI MboII MnlI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PstI PvuII Sau3AI
Sau96I ScrFI SfcI TseI Tsp45I
TspRI