Restriction map for
pF1KB5251
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# NO CUT ENZYMES ( 158 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AfaI AflII AgeI
AhdI Alw44I AlwI AocI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AviII AvrII BamHI BbeI BbrPI
BcgI BclI BfrI BglII BlnI
BlpI Bpu1102I BsaBI BsaHI BsaI
BsaWI BseAI BsgI BsiEI BsiHKAI
BsiWI BsmAI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrFI BsrGI BsrI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstXI BstYI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DpnII DraI
DraIII DrdI DsaI EagI Eam1105I
EarI EclXI Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HgaI Hin1I HpaI
KasI KpnI Ksp632I KspI MamI
MboI MfeI MflI MluI MroI
MslI MunI MvnI NaeI NarI
NcoI NdeII NgoMIV NheI NotI
NruI NsiI NspV PacI PflFI
PinAI PleI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsaI RsrII SacI SacII SalI
SapI Sau3AI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI SspBI SspI StuI
SwaI Tth111I XbaI XcmI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccI 118 GTCTAC GT^[AC][GT]AC
AflIII 122 ACGTGT A^C[GA][CT]GT
Aor51HI 75 AGCGCT AGC^GCT
AvaI 454 CTCGAG C^[CT]CG[GA]G
BalI 80 TGGCCA TGG^CCA
BanI 742 GGCACC G^G[CT][GA]CC
BanII 787 GGGCTC G[GA]GC[CT]^C
BbsI 385 GAAGAC GAAGAC(2/6)
BbvCI 723 GCTGAGG CCTCAGC(-5/-2)
BciVI 329 GTATCC GTATCC(6/5)
BcnI 245 CCCGG CC^[GC]GG
BfaI 52 CTAG C^TAG
BmyI 787 GGGCTC G[AGT]GC[ACT]^C
BpuAI 385 GAAGAC GAAGAC(2/6)
BsaAI 121 TACGTG [CT]AC^GT[GA]
BseRI 565 GAGGAG GAGGAG(10/8)
BsoBI 454 CTCGAG C^[CT]CG[GA]G
Bsp1286I 787 GGGCTC G[AGT]GC[ACT]^C
BspMI 570 GCAGGT ACCTGC(4/8)
BsrDI 507 CATTGC GCAATG(2/0)
BstAPI 39 GCAATTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Eco47III 75 AGCGCT AGC^GCT
EcoT14I 65 CCAAGG C^C[AT][AT]GG
FauI 477 GCGGG CCCGC(4/6)
HincII 460 GTTGAC GT[CT]^[GA]AC
HindII 460 GTTGAC GT[CT]^[GA]AC
HindIII 283 AAGCTT A^AGCTT
HinfI 249 GAATC G^A[ACGT]TC
MaeI 52 CTAG C^TAG
MluNI 80 TGGCCA TGG^CCA
MscI 80 TGGCCA TGG^CCA
NciI 245 CCCGG CC^[GC]GG
NdeI 335 CATATG CA^TATG
NlaIII 144 CATG CATG^
NspI 143 ACATGC [GA]CATG^[CT]
PaeR7I 454 CTCGAG C^TCGAG
PstI 520 CTGCAG CTGCA^G
SbfI 519 CCTGCAGG CCTGCA^GG
SfaNI 470 GCATC GCATC(5/9)
SmlI 454 CTCGAG C^T[CT][GA]AG
Sse8387I 519 CCTGCAGG CCTGCA^GG
StyI 65 CCAAGG C^C[AT][AT]GG
TfiI 249 GAATC G^A[AT]TC
Tsp509I 41 AATT ^AATT
XhoI 454 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
BglI 55 GCCAAGTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
64 GCCAAGGGGGC
BpmI 103 CTGGAG CTGGAG(16/14)
349 CTGGAG
BsmFI 217 GGGAC GGGAC(10/14)
479 GGGAC
BstF5I 90 CATCC GGATG(2/0)
483 CATCC
EaeI 62 CGGCCA [CT]^GGCC[GA]
80 TGGCCA
FokI 90 CATCC GGATG(9/13)
483 CATCC
HapII 192 CCGG C^CGG
246 CCGG
HpaII 192 CCGG C^CGG
246 CCGG
MaeII 122 ACGT A^CGT
416 ACGT
MseI 647 TTAA T^TAA
659 TTAA
MspI 192 CCGG C^CGG
246 CCGG
PflMI 733 CCAGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
803 CCAAGATTTGG
PpuMI 494 AGGACCT [GA]G^G[AT]CC[CT]
698 AGGACCC
PvuII 379 CAGCTG CAG^CTG
604 CAGCTG
SfcI 465 CTACAG C^T[GA][CT]AG
520 CTGCAG
Tru9I 647 TTAA T^TAA
659 TTAA
Van91I 733 CCAGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
803 CCAAGATTTGG
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# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AciI AluI AlwNI AvaII BbvI
BsaJI BsiYI BslI BssKI BstNI
Cac8I CfoI Cfr13I DdeI DraII
EcoO109I EcoRII Fnu4HI HaeII HaeIII
HhaI HinP1I HphI ItaI MaeIII
MboII MnlI MspA1I MvaI MwoI
NlaIV Sau96I ScrFI TaqI TseI
Tsp45I TspRI TthHB8I