Restriction map for
pF1KB5220
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# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
Asp718I AspEI AspHI AspI AviII
AvrII BamHI BanII BbeI BcgI
BciVI BclI BfrI BglI BlnI
BlpI BmyI Bpu1102I BsaBI BsaHI
BsaI BseAI BsgI BsiEI BsiHKAI
BsiWI BsmBI Bsp1286I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
CelII ClaI CpoI DraI DraII
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO109I EcoO65I
EcoRI FauI FbaI FseI FspI
Hin1I HpaI KasI KpnI KspI
MamI MfeI MluI MroI MspA1I
MunI MvnI NarI NheI NotI
NruI NspV PacI PaeR7I PflFI
PflMI PinAI PmeI PpuMI PshAI
Psp1406I PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SwaI Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 59 enzymes) #
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AciI 409 GCGG CCGC(-3/-1)
AcsI 936 AAATTC [GA]^AATT[CT]
AflIII 1958 ACGTGT A^C[GA][CT]GT
AocI 926 CCTTAGG CC^T[ACGT]AGG
ApoI 936 AAATTC [GA]^AATT[CT]
AseI 1051 ATTAAT AT^TAAT
AsnI 1051 ATTAAT AT^TAAT
AvaI 1799 CTCGGG C^[CT]CG[GA]G
BalI 1469 TGGCCA TGG^CCA
BanI 59 GGTGCC G^G[CT][GA]CC
BbrPI 1957 CACGTG CAC^GTG
BbvCI 1477 GCTGAGG CCTCAGC(-5/-2)
BcnI 1755 CCGGG CC^[GC]GG
BsaAI 1957 CACGTG [CT]AC^GT[GA]
BsaWI 304 ACCGGA [AT]^CCGG[AT]
BsmAI 1249 GTCTC GTCTC(1/5)
BsmFI 280 GGGAC GGGAC(10/14)
BsmI 685 GAATGC GAATGC(1/-1)
BsoBI 1799 CTCGGG C^[CT]CG[GA]G
Bsp1407I 141 TGTACA T^GTACA
BsrFI 62 GCCGGC [GA]^CCGG[CT]
BsrGI 141 TGTACA T^GTACA
BstNI 1431 CCTGG CC^[AT]GG
Bsu36I 926 CCTTAGG CC^T[ACGT]AGG
CfoI 795 GCGC GCG^C
Cfr10I 62 GCCGGC [GA]^CCGG[CT]
DsaI 326 CCATGG C^C[GA][CT]GG
EaeI 1469 TGGCCA [CT]^GGCC[GA]
EarI 1180 GAAGAG CTCTTC(1/4)
Eco81I 926 CCTTAGG CC^T[ACGT]AGG
EcoRII 1431 CCTGG ^CC[AT]GG
EcoRV 1150 GATATC GAT^ATC
EcoT22I 715 ATGCAT ATGCA^T
HaeII 794 AGCGCC [GA]GCGC^[CT]
HhaI 795 GCGC GCG^C
HinP1I 795 GCGC G^CGC
HincII 1459 GTTGAC GT[CT]^[GA]AC
HindII 1459 GTTGAC GT[CT]^[GA]AC
HindIII 1117 AAGCTT A^AGCTT
Ksp632I 1180 GAAGAG CTCTTC(1/4)
MaeII 1958 ACGT A^CGT
MluNI 1469 TGGCCA TGG^CCA
MscI 1469 TGGCCA TGG^CCA
MvaI 1431 CCTGG CC^[AT]GG
MwoI 1015 GCCAATCGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NaeI 62 GCCGGC GCC^GGC
NciI 1755 CCGGG CC^[GC]GG
NcoI 326 CCATGG C^CATGG
NdeI 293 CATATG CA^TATG
NgoMIV 62 GCCGGC G^CCGGC
NsiI 715 ATGCAT ATGCA^T
PmaCI 1957 CACGTG CAC^GTG
PmlI 1957 CACGTG CAC^GTG
PshBI 1051 ATTAAT AT^TAAT
PstI 2101 CTGCAG CTGCA^G
SmlI 1561 CTTGAG C^T[CT][GA]AG
SspBI 141 TGTACA T^GTACA
StuI 1725 AGGCCT AGG^CCT
Tsp45I 473 GTGAC ^GT[GC]AC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AlwNI 1142 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG
1910 CAGTACCTG
AvaII 202 GGACC G^G[AT]CC
1947 GGACC
BbsI 1843 GAAGAC GAAGAC(2/6)
911 GTCTTC
BglII 747 AGATCT A^GATCT
1377 AGATCT
BpmI 779 CTGGAG CTGGAG(16/14)
1732 CTGGAG
BpuAI 1843 GAAGAC GAAGAC(2/6)
911 GTCTTC
BseRI 1291 GAGGAG GAGGAG(10/8)
1978 GAGGAG
BsiYI 523 CCTGATGTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
2044 CCAAAGACAGG
BslI 523 CCTGATGTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
2044 CCAAAGACAGG
BssKI 1431 CCTGG ^CC[ACGT]GG
1755 CCGGG
HphI 415 GGTGA GGTGA(8/7)
1186 GGTGA
MslI 876 CACTCATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1834 CATTGGAATG
NlaIV 59 GGTGCC GG[ACGT]^[ACGT]CC
65 GGCCCC
NspI 713 ACATGC [GA]CATG^[CT]
717 GCATGT
ScrFI 1431 CCTGG CC^[ACGT]GG
1755 CCGGG
SfcI 680 CTATAG C^T[GA][CT]AG
2101 CTGCAG
SspI 961 AATATT AAT^ATT
1030 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AfaI AluI AlwI Asp700I BbvI
BfaI BsaJI BsrI BstF5I BstYI
Cac8I Cfr13I DdeI DpnII EcoT14I
Fnu4HI FokI HaeIII HapII HgaI
HinfI HpaII ItaI MaeI MaeIII
MboI MboII MflI MnlI MseI
MspI NdeII NlaIII PleI RsaI
Sau3AI Sau96I SfaNI StyI TaqI
TfiI Tru9I TseI Tsp509I TspRI
TthHB8I XhoII XmnI