Restriction map for
pF1KB5188
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# NO CUT ENZYMES ( 124 enzymes) #
##################################
Acc65I AclI AcsI AflII AflIII
AgeI AhdI AocI Aor51HI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AviII AvrII
BbeI BbrPI BbvCI BciVI BclI
BfaI BfrI BlnI BsaBI BsiWI
BsmI BsoBI BspDI BspLU11I BspMI
BsrBI BssHII BssSI Bst1107I BstBI
BstZ17I Bsu36I ClaI CpoI DraI
DraIII EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoRI EcoRV
EcoT22I FbaI FseI FspI HgaI
HincII HindII HindIII HpaI KasI
KpnI KspI MaeI MamI MfeI
MluI MseI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI Sse8387I
SspI StuI SwaI TaqI TfiI
Tru9I Tsp509I Tth111I TthHB8I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AatII 912 GACGTC GACGT^C
AccI 1075 GTCTAC GT^[AC][GT]AC
AccIII 668 TCCGGA T^CCGGA
AcyI 912 GACGTC G[GA]^CG[CT]C
Alw44I 552 GTGCAC G^TGCAC
ApaI 710 GGGCCC GGGCC^C
ApaLI 552 GTGCAC G^TGCAC
BamHI 896 GGATCC G^GATCC
BcgI 974 GCACAGCCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 112 CCGGG CC^[GC]GG
BglI 359 GCCTCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 471 AGATCT A^GATCT
BlpI 167 GCTCAGC GC^T[ACGT]AGC
BpmI 132 CTGGAG CTGGAG(16/14)
Bpu1102I 167 GCTCAGC GC^T[ACGT]AGC
BsaAI 889 TACGTG [CT]AC^GT[GA]
BsaHI 912 GACGTC G[GA]^CG[CT]C
BsaI 544 GAGACC GGTCTC(1/5)
BsaWI 668 TCCGGA [AT]^CCGG[AT]
BseAI 668 TCCGGA T^CCGGA
BsmBI 1153 GAGACG CGTCTC(1/5)
Bsp1407I 1136 TGTACA T^GTACA
BspEI 668 TCCGGA T^CCGGA
BspHI 500 TCATGA T^CATGA
BsrDI 521 GCAATG GCAATG(2/0)
BsrFI 193 ACCGGC [GA]^CCGG[CT]
BsrGI 1136 TGTACA T^GTACA
BstEII 966 GGTGACC G^GT[ACGT]ACC
BstPI 966 GGTGACC G^GT[ACGT]ACC
CelII 167 GCTCAGC GC^T[ACGT]AGC
Cfr10I 193 ACCGGC [GA]^CCGG[CT]
DraII 710 GGGCCCT [GA]G^G[ACGT]CC[CT]
DrdI 1038 GACCCAGTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 654 CTCTTC CTCTTC(1/4)
EcoO109I 710 GGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 966 GGTGACC G^GT[ACGT]ACC
HaeII 128 GGCGCT [GA]GCGC^[CT]
Hin1I 912 GACGTC G[GA]^CG[CT]C
HinfI 755 GACTC G^A[ACGT]TC
Ksp632I 654 CTCTTC CTCTTC(1/4)
MaeIII 967 GTGAC ^GT[ACGT]AC
MroI 668 TCCGGA T^CCGGA
NciI 112 CCGGG CC^[GC]GG
NspI 491 GCATGC [GA]CATG^[CT]
PflMI 510 CCAGCTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 755 GACTC GAGTC(4/5)
RcaI 500 TCATGA T^CATGA
SacI 165 GAGCTC GAGCT^C
SphI 491 GCATGC GCATG^C
SspBI 1136 TGTACA T^GTACA
Tsp45I 967 GTGAC ^GT[GC]AC
Van91I 510 CCAGCTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 353 CCATCTGCCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 21 enzymes) #
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AccII 396 CGCG CG^CG
1210 CGCG
AfaI 837 GTAC GT^AC
1137 GTAC
BanII 165 GAGCTC G[GA]GC[CT]^C
710 GGGCCC
BbsI 79 GAAGAC GAAGAC(2/6)
997 GTCTTC
BpuAI 79 GAAGAC GAAGAC(2/6)
997 GTCTTC
BsgI 418 CTGCAC GTGCAG(16/14)
776 GTGCAG
BsiEI 191 CGACCG CG[GA][CT]^CG
398 CGGTCG
BsmAI 544 GAGAC GTCTC(1/5)
1153 GAGAC
BstAPI 56 GCAGCGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
350 GCACCATCTGC
BstUI 396 CGCG CG^CG
1210 CGCG
BstXI 986 CCATCTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1094 CCATCAGCTTGG
BstYI 471 AGATCT [GA]^GATC[CT]
896 GGATCC
EcoT14I 569 CCATGG C^C[AT][AT]GG
614 CCATGG
MflI 471 AGATCT [GA]^GATC[CT]
896 GGATCC
MvnI 396 CGCG CG^CG
1210 CGCG
NcoI 569 CCATGG C^CATGG
614 CCATGG
PstI 901 CTGCAG CTGCA^G
1190 CTGCAG
PvuII 1033 CAGCTG CAG^CTG
1116 CAGCTG
RsaI 837 GTAC GT^AC
1137 GTAC
StyI 569 CCATGG C^C[AT][AT]GG
614 CCATGG
XhoII 471 AGATCT [GA]^GATC[CT]
896 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AciI AluI AlwI AlwNI AspHI
AvaII BalI BanI BbvI BmyI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII DsaI EaeI EcoRII FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HpaII HphI ItaI MaeII
MboI MboII MluNI MnlI MscI
MslI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV Sau3AI Sau96I
ScrFI SfaNI SfcI TseI TspRI