Restriction map for
pF1KB4990
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# NO CUT ENZYMES ( 115 enzymes) #
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AatII Acc65I AccI AclI AcsI
AfaI AflII AflIII AgeI AhdI
Alw44I ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AspI AvrII
BalI BbeI BbrPI BciVI BclI
BfrI BlnI BsaAI BsaBI BsaI
BsiEI BsiWI Bsp1407I BspDI BspLU11I
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstZ17I ClaI CpoI
DraI DraIII DrdI EaeI EagI
Eam1105I EclXI Eco52I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI HincII
HindII HindIII HpaI KasI KpnI
KspI MamI MfeI MluI MluNI
MscI MseI MunI NaeI NarI
NdeI NgoMIV NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RsaI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tru9I Tsp509I Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 38 enzymes) #
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Aor51HI 27 AGCGCT AGC^GCT
Asp700I 1181 GAATCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 395 CCCGGG C^[CT]CG[GA]G
BbsI 1242 GAAGAC GAAGAC(2/6)
BcgI 1201 GCACTGGAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 1123 GCCAAGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 548 AGATCT A^GATCT
BlpI 939 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 939 GCTGAGC GC^T[ACGT]AGC
BpuAI 1242 GAAGAC GAAGAC(2/6)
BseRI 535 CTCCTC GAGGAG(10/8)
BsmBI 1114 CGTCTC CGTCTC(1/5)
BsmI 455 GAATGC GAATGC(1/-1)
BsoBI 395 CCCGGG C^[CT]CG[GA]G
BspHI 656 TCATGA T^CATGA
BsrDI 1053 CATTGC GCAATG(2/0)
BsrFI 1424 ACCGGC [GA]^CCGG[CT]
BstAPI 858 GCAGCTGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 939 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1424 ACCGGC [GA]^CCGG[CT]
DsaI 827 CCATGG C^C[GA][CT]GG
Eco47III 27 AGCGCT AGC^GCT
EcoNI 654 CCTCATGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
MaeII 508 ACGT A^CGT
MslI 1098 CATGACTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 827 CCATGG C^CATGG
NheI 1042 GCTAGC G^CTAGC
PflMI 888 CCACGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 983 GGGTCCT [GA]G^G[AT]CC[CT]
PshAI 1108 GACGCCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 656 TCATGA T^CATGA
SapI 778 GAAGAGC GCTCTTC(1/4)
SmaI 395 CCCGGG CCC^GGG
StuI 440 AGGCCT AGG^CCT
Van91I 888 CCACGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 395 CCCGGG C^CCGGG
XmaI 395 CCCGGG C^CCGGG
XmnI 1181 GAATCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 22 enzymes) #
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AccIII 153 TCCGGA T^CCGGA
302 TCCGGA
AcyI 1108 GACGCC G[GA]^CG[CT]C
1443 GGCGTC
AlwNI 256 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1469 CAGCAGCTG
ApaI 14 GGGCCC GGGCC^C
851 GGGCCC
AspHI 235 GAGCTC G[AT]GC[AT]^C
388 GAGCTC
BamHI 399 GGATCC G^GATCC
663 GGATCC
BanI 882 GGTGCC G^G[CT][GA]CC
1021 GGCACC
BfaI 25 CTAG C^TAG
1043 CTAG
BsaHI 1108 GACGCC G[GA]^CG[CT]C
1443 GGCGTC
BsaWI 153 TCCGGA [AT]^CCGG[AT]
302 TCCGGA
BseAI 153 TCCGGA T^CCGGA
302 TCCGGA
BsiHKAI 235 GAGCTC G[AT]GC[AT]^C
388 GAGCTC
BspEI 153 TCCGGA T^CCGGA
302 TCCGGA
BsrBI 781 GAGCGG CCGCTC(-3/-3)
1282 GAGCGG
Hin1I 1108 GACGCC G[GA]^CG[CT]C
1443 GGCGTC
MaeI 25 CTAG C^TAG
1043 CTAG
MroI 153 TCCGGA T^CCGGA
302 TCCGGA
NspI 45 ACATGC [GA]CATG^[CT]
899 ACATGC
SacI 235 GAGCTC GAGCT^C
388 GAGCTC
TaqI 239 TCGA T^CGA
818 TCGA
TfiI 1181 GAATC G^A[AT]TC
1361 GATTC
TthHB8I 239 TCGA T^CGA
818 TCGA
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# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AccII AciI AluI AlwI AocI
AvaII AviII BanII BbvCI BbvI
BcnI BmyI BpmI BsaJI BsgI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraII
EarI Eco81I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI FspI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeIII MboI MboII MflI MnlI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
PstI PvuII Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TseI Tsp45I
TspRI XcmI XhoII