Restriction map for
pF1KB4977
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# NO CUT ENZYMES ( 154 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AvaI AviII AvrII
BamHI BanI BanII BbeI BbrPI
BbsI BbvCI BbvI BcgI BciVI
BclI BcnI BfrI BglI BglII
BlnI BpuAI BsaHI BsaI BseAI
BseRI BsgI BsiEI BsiHKAI BsiWI
BsmFI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I ClaI CpoI DraI DraII
DraIII DrdI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HaeII Hin1I
HindIII HpaI KasI KpnI Ksp632I
KspI MfeI MluI MroI MspA1I
MunI MvnI NarI NciI NdeI
NheI NlaIV NotI NruI NsiI
NspV PacI PaeR7I PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SmaI SnaBI SpeI SphI
Sse8387I SspBI StuI SwaI TseI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AccI 18 GTCGAC GT^[AC][GT]AC
AcsI 589 AAATTC [GA]^AATT[CT]
AlwNI 61 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 589 AAATTC [GA]^AATT[CT]
AspI 118 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 455 GGACC G^G[AT]CC
BalI 561 TGGCCA TGG^CCA
BfaI 503 CTAG C^TAG
BlpI 66 GCTGAGC GC^T[ACGT]AGC
BmyI 700 GGGCAC G[AGT]GC[ACT]^C
BpmI 101 CTGGAG CTGGAG(16/14)
Bpu1102I 66 GCTGAGC GC^T[ACGT]AGC
BsaAI 412 TACGTG [CT]AC^GT[GA]
BsaJI 564 CCATGG C^C[ACGT][ACGT]GG
BsaWI 395 TCCGGT [AT]^CCGG[AT]
BsiYI 283 CCATTGTTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 283 CCATTGTTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmBI 104 GAGACG CGTCTC(1/5)
BsmI 813 GAATGC GAATGC(1/-1)
Bsp1286I 700 GGGCAC G[AGT]GC[ACT]^C
BsrFI 27 GCCGGC [GA]^CCGG[CT]
BstAPI 1119 GCAAAAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 66 GCTGAGC GC^T[ACGT]AGC
CfoI 25 GCGC GCG^C
Cfr10I 27 GCCGGC [GA]^CCGG[CT]
Cfr13I 455 GGACC G^G[ACGT]CC
DsaI 564 CCATGG C^C[GA][CT]GG
EcoT14I 564 CCATGG C^C[AT][AT]GG
FauI 31 GCGGG CCCGC(4/6)
Fnu4HI 49 GCGGC GC^[ACGT]GC
HgaI 118 GACGC GACGC(5/10)
HhaI 25 GCGC GCG^C
HinP1I 25 GCGC G^CGC
HincII 18 GTCGAC GT[CT]^[GA]AC
HindII 18 GTCGAC GT[CT]^[GA]AC
HphI 742 GGTGA GGTGA(8/7)
ItaI 49 GCGGC GC^[ACGT]GC
MaeI 503 CTAG C^TAG
MaeII 413 ACGT A^CGT
MluNI 561 TGGCCA TGG^CCA
MscI 561 TGGCCA TGG^CCA
NaeI 27 GCCGGC GCC^GGC
NcoI 564 CCATGG C^CATGG
NgoMIV 27 GCCGGC G^CCGGC
NspI 41 ACATGC [GA]CATG^[CT]
PflFI 118 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 283 CCATTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SalI 18 GTCGAC G^TCGAC
Sau96I 455 GGACC G^G[ACGT]CC
SgrAI 26 CGCCGGCG C[GA]^CCGG[CT]G
SmlI 262 CTCAAG C^T[CT][GA]AG
StyI 564 CCATGG C^C[AT][AT]GG
TaqI 19 TCGA T^CGA
Tth111I 118 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 19 TCGA T^CGA
Van91I 283 CCATTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 502 TCTAGA T^CTAGA
XcmI 279 CCAACCATTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AciI 31 GCGG CCGC(-3/-1)
49 GCGG
AfaI 140 GTAC GT^AC
1092 GTAC
BssKI 187 CCTGG ^CC[ACGT]GG
997 CCTGG
BstNI 187 CCTGG CC^[AT]GG
997 CCTGG
EaeI 50 CGGCCA [CT]^GGCC[GA]
561 TGGCCA
EcoRII 187 CCTGG ^CC[AT]GG
997 CCTGG
HapII 28 CCGG C^CGG
396 CCGG
HinfI 826 GAATC G^A[ACGT]TC
1039 GAATC
HpaII 28 CCGG C^CGG
396 CCGG
MspI 28 CCGG C^CGG
396 CCGG
MvaI 187 CCTGG CC^[AT]GG
997 CCTGG
MwoI 467 GCAATACGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1119 GCAAAAGATGC
RsaI 140 GTAC GT^AC
1092 GTAC
ScrFI 187 CCTGG CC^[ACGT]GG
997 CCTGG
SfcI 344 CTGTAG C^T[GA][CT]AG
491 CTGTAG
SspI 236 AATATT AAT^ATT
681 AATATT
TfiI 826 GAATC G^A[AT]TC
1039 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AluI AlwI BsaBI BsmAI BsrI
BstF5I BstYI Cac8I DdeI DpnII
FokI HaeIII MaeIII MamI MboI
MboII MflI MnlI MseI MslI
NdeII NlaIII Sau3AI SfaNI Tru9I
Tsp45I Tsp509I TspRI XhoII