Restriction map for
pF1KB4948
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# NO CUT ENZYMES ( 117 enzymes) #
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Acc65I AccII AclI AflII AflIII
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspHI AspI AviII AvrII
BbeI BbrPI BcgI BciVI BclI
BfrI BlnI BlpI Bpu1102I BsaAI
BsaI BsiEI BsiHKAI BsiWI BsmI
Bsp1407I BspHI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstUI BstZ17I CelII
Cfr10I CpoI DraI DraIII EagI
Eam1105I EclXI Eco47III Eco52I EcoRV
EcoT22I FbaI FseI FspI HgaI
HindIII HpaI KasI KpnI KspI
MfeI MluI MunI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfoI SfuI
SgrAI SmlI SnaBI SpeI Sse8387I
SspBI SspI SwaI TfiI Tth111I
XbaI XhoI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AatII 17 GACGTC GACGT^C
AccI 592 GTCTAC GT^[AC][GT]AC
AccIII 1417 TCCGGA T^CCGGA
AcyI 17 GACGTC G[GA]^CG[CT]C
Asp700I 315 GAAGTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 1463 CCCGGG C^[CT]CG[GA]G
BsaBI 348 GATTGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 17 GACGTC G[GA]^CG[CT]C
BsaWI 1417 TCCGGA [AT]^CCGG[AT]
BseAI 1417 TCCGGA T^CCGGA
BsgI 1540 GTGCAG GTGCAG(16/14)
BsmBI 19 CGTCTC CGTCTC(1/5)
BsoBI 1463 CCCGGG C^[CT]CG[GA]G
BspDI 289 ATCGAT AT^CGAT
BspEI 1417 TCCGGA T^CCGGA
BstEII 1439 GGTCACC G^GT[ACGT]ACC
BstPI 1439 GGTCACC G^GT[ACGT]ACC
CfoI 1207 GCGC GCG^C
ClaI 289 ATCGAT AT^CGAT
EcoO65I 1439 GGTCACC G^GT[ACGT]ACC
EcoRI 373 GAATTC G^AATTC
HaeII 1206 AGCGCC [GA]GCGC^[CT]
HhaI 1207 GCGC GCG^C
Hin1I 17 GACGTC G[GA]^CG[CT]C
HinP1I 1207 GCGC G^CGC
HincII 574 GTCAAC GT[CT]^[GA]AC
HindII 574 GTCAAC GT[CT]^[GA]AC
MaeII 18 ACGT A^CGT
MamI 348 GATTGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1417 TCCGGA T^CCGGA
MslI 810 CACCAGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
SfiI 207 GGCCCCTCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 1463 CCCGGG CCC^GGG
SphI 498 GCATGC GCATG^C
XmaCI 1463 CCCGGG C^CCGGG
XmaI 1463 CCCGGG C^CCGGG
XmnI 315 GAAGTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 28 enzymes) #
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AcsI 3 GAATTT [GA]^AATT[CT]
373 GAATTC
ApoI 3 GAATTT [GA]^AATT[CT]
373 GAATTC
BanI 128 GGCACC G^G[CT][GA]CC
1507 GGTGCC
BanII 1229 GGGCTC G[GA]GC[CT]^C
1407 GAGCCC
BbsI 523 GAAGAC GAAGAC(2/6)
1675 GAAGAC
BfaI 391 CTAG C^TAG
1285 CTAG
BglI 208 GCCCCTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1048 GCCGTCAAGGC
BglII 638 AGATCT A^GATCT
1354 AGATCT
BmyI 1229 GGGCTC G[AGT]GC[ACT]^C
1407 GAGCCC
BpuAI 523 GAAGAC GAAGAC(2/6)
1675 GAAGAC
Bsp1286I 1229 GGGCTC G[AGT]GC[ACT]^C
1407 GAGCCC
BstXI 885 CCAGAAAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1350 CCAGAGATCTGG
DrdI 424 GACATGCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1159 GACCTTGTTGTC
EarI 1135 GAAGAG CTCTTC(1/4)
1570 GAAGAG
EcoNI 1279 CCTCAGCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1309 CCTCCGCAAGG
FauI 112 CCCGC CCCGC(4/6)
669 CCCGC
HinfI 862 GACTC G^A[ACGT]TC
1573 GAGTC
Ksp632I 1135 GAAGAG CTCTTC(1/4)
1570 GAAGAG
MaeI 391 CTAG C^TAG
1285 CTAG
MseI 635 TTAA T^TAA
1241 TTAA
PflMI 135 CCAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
645 CCATTCCCTGG
PleI 862 GACTC GAGTC(4/5)
1573 GAGTC
PstI 550 CTGCAG CTGCA^G
603 CTGCAG
StuI 851 AGGCCT AGG^CCT
1493 AGGCCT
Tru9I 635 TTAA T^TAA
1241 TTAA
TspRI 192 CAGTG CA[GC]TG[ACGT][ACGT]^
1473 CAGTG
Van91I 135 CCAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
645 CCATTCCCTGG
XcmI 272 CCAGGATCCTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1062 CCACAGCAAGGATGG
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# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AocI AvaII BalI BamHI BbvCI
BbvI BcnI BpmI BsaJI BseRI
BsiYI BslI BsmAI BsmFI BspMI
BsrI BssKI BstF5I BstNI BstYI
Bsu36I Cac8I Cfr13I DdeI DpnII
DraII DsaI EaeI Eco81I EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HpaII HphI ItaI MaeIII
MboI MboII MflI MluNI MnlI
MscI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PpuMI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TseI Tsp45I Tsp509I TthHB8I
XhoII